Rubra_00120 Rubra_00120   Rubra_00140 Rubra_00140

Rubra_00130 : CDS information

close this sectionLocation

Organism
StrainNRRL 3061 (=NBRC 14000)
Entry nameRubradirin
Contig
Start / Stop / Direction29,116 / 29,739 / + [in whole cluster]
29,116 / 29,739 / + [in contig]
Location29116..29739 [in whole cluster]
29116..29739 [in contig]
TypeCDS
Length624 bp (207 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.2 modification methylation
Productputative methyltransferase
Product (GenBank)hypothetical protein
Gene
Gene (GenBank)
EC number2.1.1.-
Keyword
Note
Note (GenBank)
  • ORF6
Reference
ACC
PmId
[18080113] Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061. (Arch Microbiol. , 2008)
comment
Rubradirin生合成clusterの報告。

table1>配列比較からのProposed function
ORF6(207aa): Methyltransferase

本文に詳細記載なし
Related Reference
ACC
Q7X2F7
NITE
Gilvo_00070
PmId
[22800463] Roles of the synergistic reductive O-methyltransferase GilM and of O-methyltransferase GilMT in the gilvocarcin biosynthetic pathway. (J Am Chem Soc. , 2012)
comment
Blast 33rd, id37%, 3e-21
Streptomyces griseoflavus_gilM
Putative uncharacterized protein gilM
[DoBISCUIT]oxidoreductase/C-12 O-methyltransferase

GilMT and GilMの調査。
recombinant enzymesを使った合成中間体とのin vitro実験。

GilMT反応由来の産物5とGilMを反応させて産物を解析。
GilMはgilvocarcinsのtetracyclic coreを構築するために一連の反応を触媒する。

産物5
↓quinone還元
↓hemiacetal形成
demethyl-defuco-pregilvocarcin M
↓O-methylation
defuco-pregilvocarcin M

「GilMはGilRの反応によるFADH2供給を受けて還元的触媒を媒介する。
GilMの反応により、FADH2はGilRのためのFADへとリサイクルされる。」
と仮定し、defuco-gilvocarcin Mの産生量が産物5 + GilMより、産物5 + GilM + GilRで10倍増えることから、GilMはGilRと相乗的に働くことが確認されたと述べている。

この珍しい相乗効果により、polyketide由来defuco-gilvocarcin chromphoreの生合成は最終的に完了する。

defuco-pregilvocarcin M
↓GilR: FAD-dependent oxidoreductase
defuco-gilvocarcin M

close this sectionSequence

selected fasta
>putative methyltransferase [hypothetical protein]
MGGSKVEGFDAHYAGTPLWDLGRPQTALRDLAEAGAFRGRVLDVGCGTGEVALMAAALGL
PTVGIDSASTAIGIARRKAEERGLPARFLVGDALNSADLGEQFDTVLDCALFHAFSETER
VRYAGSLATVMPPDARLFLLCISDRHHPGSGPRRESQGGIRARRVSQDEIRATFADGWRV
DSIEPATLENNRDIDGAPGWLAAITRT
selected fasta
>putative methyltransferase [hypothetical protein]
ATGGGTGGCAGCAAGGTGGAGGGCTTCGACGCGCACTACGCCGGAACACCGCTCTGGGAC
CTCGGCCGCCCCCAGACGGCCCTGCGCGACCTCGCCGAGGCGGGCGCCTTCCGCGGCCGG
GTGCTCGACGTCGGGTGCGGCACCGGCGAGGTCGCGCTCATGGCGGCGGCCCTCGGCCTG
CCGACGGTCGGCATCGACTCGGCGTCGACGGCGATCGGGATCGCGCGGCGCAAAGCGGAA
GAGCGCGGCCTGCCGGCGCGCTTCCTGGTCGGGGACGCACTCAACTCGGCGGACTTGGGC
GAGCAGTTCGACACCGTGCTCGACTGCGCCCTGTTCCACGCGTTCAGCGAGACCGAACGC
GTCCGCTACGCCGGCAGCCTGGCCACCGTGATGCCGCCGGACGCGCGGTTGTTCCTGCTC
TGCATCAGCGACCGCCATCACCCCGGCAGCGGACCGCGACGGGAGAGCCAGGGCGGGATC
CGCGCCAGGCGGGTGAGCCAGGACGAAATCCGGGCCACCTTCGCCGACGGCTGGCGGGTC
GATTCGATCGAACCCGCGACATTGGAGAACAACAGAGACATCGACGGGGCACCGGGCTGG
CTGGCCGCGATCACCCGCACCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP No significant hit
TMHMM No significant hit
Rubra_00120 Rubra_00120   Rubra_00140 Rubra_00140
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