Soraph_00050 Soraph_00050   Soraph_00070 Soraph_00070

Soraph_00060 : CDS information

close this sectionLocation

Organism
StrainSo ce26
Entry nameSoraphen
Contig
Start / Stop / Direction13,697 / 14,158 / + [in whole cluster]
13,697 / 14,158 / + [in contig]
Location13697..14158 [in whole cluster]
13697..14158 [in contig]
TypeCDS
Length462 bp (153 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative oxidoreductase
Product (GenBank)reductase
Gene
Gene (GenBank)sorR
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[12039053] Characterization of the biosynthetic gene cluster for the antifungal polyketide soraphen A from Sorangium cellulosum So ce26. (Gene. , 2002)
[15289572] Heterologous production of the antifungal polyketide antibiotic soraphen A of Sorangium cellulosum So ce26 in Streptomyces lividans. (Microbiology. , 2004)
comment
[PMID: 12039053](2002)
Soraphen A biosynthetic gene clusterの報告。
SorR(170aa)は配列解析からreductaseであると提唱。

aglycon3位と7位での架橋構造を形成するためにC-3 keto基をもたらすoxidaseとして機能するか、C-9 and C-10間の二重結合をもたらすoxidoreductaseであると提唱されている。
ORF4も同じ役割をする酵素の候補である。

---
[PMID: 15289572](2004)
S. lividansでのsor genes異種性発現。

SorABRCDFE株 + cinnamate
SorABRCDFE株 + badA(benzoate-CoA ligase) + benzoate or cinnamate
の組み合わせでSoraphen A産生。

SorABRCDFE株で発現されているのは、the sorA, and the sorB4M, sorRCDFE operons.
Family/Domain
FD
IPR013968
comment
[IPR013968] Polyketide synthase, KR (Domain)

close this sectionSequence

selected fasta
>putative oxidoreductase [reductase]
MSVIHHFFTRPTPIRDAPDTYPSSERASMLAQVRSGCTPMVVGVDAFLLFSSSAGVLGSG
LQGAYSAANAFLDVLSEQPRVLGRSATSIAWCIWNGGGIVDHAGKEHLRRRGVSVMPLDL
ALAASYREESRGGSPQRSSPSLCSPVPSSRCPG
selected fasta
>putative oxidoreductase [reductase]
ATGAGTGTAATTCACCATTTCTTCACGCGCCCGACACCTATCCGAGACGCGCCCGACACC
TATCCGAGCTCGGAAAGAGCGTCCATGCTCGCGCAGGTTCGATCAGGCTGCACGCCCATG
GTGGTCGGTGTAGACGCCTTCCTCCTCTTCTCCTCGAGTGCAGGTGTCTTGGGGAGCGGG
CTGCAGGGCGCCTACTCGGCGGCCAACGCTTTCCTGGACGTGCTGTCCGAGCAGCCTCGC
GTCCTCGGACGGTCGGCCACGTCGATTGCCTGGTGCATATGGAACGGCGGAGGTATTGTC
GACCACGCAGGCAAAGAGCATCTCAGAAGACGTGGCGTGTCCGTGATGCCGCTAGATCTC
GCCCTCGCGGCCTCGTATCGTGAGGAATCCAGGGGTGGGAGCCCGCAAAGATCCTCACCC
TCACTCTGCTCGCCCGTGCCATCGAGCCGTTGTCCGGGATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR013968 Polyketide synthase, KR (Domain)
[44-96] 1.7e-15 PF08659
PF08659   KR
IPR016040 NAD(P)-binding domain (Domain)
[47-101] 8.69999999999999e-06 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-16] 0.253 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Soraph_00050 Soraph_00050   Soraph_00070 Soraph_00070
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