Spino_00020 Spino_00020   Spino_00040 Spino_00040

Spino_00030 : CDS information

close this sectionLocation

Organism
StrainNRRL 18538
Entry nameSpinosyn
Contig
Start / Stop / Direction4,165 / 3,416 / - [in whole cluster]
4,165 / 3,416 / - [in contig]
Locationcomplement(3416..4165) [in whole cluster]
complement(3416..4165) [in contig]
TypeCDS
Length750 bp (249 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductNDP-hexose N,N-dimethyltransferase
Product (GenBank)probable N-dimethyltransferase
Gene
Gene (GenBank)spnS
EC number2.1.1.-
Keyword
  • D-forosamine
Note
Note (GenBank)
  • probably involved in forosamine biosynthesis
Reference
ACC
PmId
[11358695] Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa. (Chem Biol. , 2001)
[11386361] A cluster of genes for the biosynthesis of spinosyns, novel macrolide insect control agents produced by Saccharopolyspora spinosa. (Antonie Van Leeuwenhoek. , 2000)
[18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008)
comment
[PMID:11358695](2001)
spinosad biosynthetic gene clusterの報告。
spnS(250aa): forosamine; dimethyltransferase

--
[PMID:11386361](2000)
同じ著者らの先行論文。
spaS(spnS?): Forosamine synthesis

--
[PMID: 18345667](2008)
D-forosamine生合成に関連するspnO, N, Q, R, and Sのクローニング、特徴づけ。
使用している株はNRRL 18537.

dTDP-D-forosamine生合成での最終ステップを触媒するN,N-dimethyltransferaseとしてのSpnSの機能を確立するため、SpnSをE.coliで発現、精製、タンパク確認、酵素活性確認。
十分な量の予測基質を用意できないため、phosphonate mimicを基質としてmonomethylated, dimethylated産物が得られることを確認。

close this sectionSequence

selected fasta
>NDP-hexose N,N-dimethyltransferase [probable N-dimethyltransferase]
MSRVSDTFAETSSVYSPDHADIYDAIHSARGRDWAAEAGEVVQLVRTRLPEAQSLLDVAC
GTGAHLERFRAEYAKVAGLELSDAMREIAIRRVPEVPIHIGDIRDFDLGEPFDVITCLCF
TAAYMRTVDDLRRVTRNMARHLAPGGVAVIEPWWFPDKFIDGFVTGAVAHHGERVISRLS
HSVLEGRTSRMTVRYTVAEPTGIRDFTEFEILSLFTEDEYTAALEDAGIRAEYLPGAPNG
RGLFVGIRN
selected fasta
>NDP-hexose N,N-dimethyltransferase [probable N-dimethyltransferase]
ATGTCGCGCGTGAGCGACACATTCGCAGAAACCTCCTCGGTATACAGCCCAGATCATGCC
GACATCTACGACGCGATCCACTCCGCGCGTGGCCGGGACTGGGCAGCCGAGGCCGGGGAA
GTAGTCCAGCTCGTACGCACCAGGCTGCCCGAAGCACAGTCCCTACTCGACGTCGCCTGT
GGGACCGGGGCGCACCTAGAGCGATTCCGTGCCGAATACGCGAAGGTCGCGGGGCTTGAA
CTGTCCGATGCGATGCGGGAGATCGCGATCAGACGAGTCCCTGAGGTACCGATTCACATC
GGTGACATCCGCGATTTCGACCTCGGCGAGCCATTCGACGTCATCACCTGCCTGTGCTTT
ACCGCGGCTTACATGCGGACCGTTGACGACCTGCGACGCGTGACGCGGAACATGGCCCGG
CACCTGGCCCCTGGTGGAGTCGCGGTCATCGAACCCTGGTGGTTTCCCGACAAGTTCATC
GACGGGTTCGTCACCGGAGCCGTCGCGCACCACGGCGAGCGGGTGATCAGCCGGCTATCG
CACTCGGTCCTGGAGGGCCGTACGAGCCGGATGACCGTCCGCTACACAGTCGCCGAACCC
ACCGGGATCCGGGACTTCACAGAGTTCGAAATCCTCTCGCTGTTCACTGAGGACGAGTAC
ACCGCCGCGCTCGAAGACGCAGGGATCCGCGCGGAATACCTTCCTGGAGCACCGAACGGC
CGAGGCCTGTTCGTCGGAATCCGCAACTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP
[1-35] 0.047 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Spino_00020 Spino_00020   Spino_00040 Spino_00040
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