Spino_00030 Spino_00030   Spino_00050 Spino_00050

Spino_00040 : CDS information

close this sectionLocation

Organism
StrainNRRL 18538
Entry nameSpinosyn
Contig
Start / Stop / Direction5,325 / 4,168 / - [in whole cluster]
5,325 / 4,168 / - [in contig]
Locationcomplement(4168..5325) [in whole cluster]
complement(4168..5325) [in contig]
TypeCDS
Length1,158 bp (385 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductNDP-hexose C-4 aminotransferase
Product (GenBank)probable aminotransferase
Gene
Gene (GenBank)spnR
EC number2.6.1.-
Keyword
  • D-forosamine
Note
Note (GenBank)
  • probably involved in forosamine biosynthesis
Reference
ACC
PmId
[11358695] Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa. (Chem Biol. , 2001)
[11386361] A cluster of genes for the biosynthesis of spinosyns, novel macrolide insect control agents produced by Saccharopolyspora spinosa. (Antonie Van Leeuwenhoek. , 2000)
[15913355] Characterization of protein encoded by spnR from the spinosyn gene cluster of Saccharopolyspora spinosa: mechanistic implications for forosamine biosynthesis. (J Am Chem Soc. , 2005)
[18345667] In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa. (J Am Chem Soc. , 2008)
comment
[PMID:11358695](2001)
spinosad biosynthetic gene clusterの報告。

spnR(386aa): forosamine; transaminase
forosamine合成において糖にアミノ基を付加する。

--
[PMID:11386361](2000)
同じ著者らの先行論文。
spnR: Forosamine synthesis

--
[PMID: 15913355](2005)abstract
spnRをE.coliで発現、精製、特徴づけ。

--
[PMID: 18345667](2008)
D-forosamine生合成に関連するspnO, N, Q, R, and Sのクローニング、特徴づけ。
使用している株はNRRL 18537.

SpnRの予測基質13は不安定なので、phosphonate mimic 24を用いて検討。
4-keto product 27が産生され、この産物はSpnRで24に変換される。
また、SpnQとの連続反応で天然基質からの反応も示されている。
C-3にOH基があってもC-4でのtransaminationができる。

close this sectionSequence

selected fasta
>NDP-hexose C-4 aminotransferase [probable aminotransferase]
MINLHQPILGTEELDAIAEVFASNWIGLGPRTRTFEAEFAHHLGVDPEQVVFLNSGTAAL
FLTVQVLDLGPGDDVVLPSISFVAAANAIASSGARPVFCDVDPRTLNPTLDDVARAITPA
TKAVLLLHYGGSPGEVTAIADFCREKGLMLIEDSACAVASSVHGTACGTFGDLATWSFDA
MKILVTGDGGMFYAADPELAHRARRLAYHGLEQMSGFDSAKSSNRWWDIRVEDIGQRLIG
NDMTAALGSVQLRKLPEFINRRREIATQYDRLLSDVPGVLLPPTLPDGHVSSHYFYWVQL
APEIRDQVAQQMLERGIYTSYRYPPLHKVPIYRADCKLPSAEDACRRTLLLPLHPSLDDA
EVRTVADEFQKAVEHHISQRSPLRK
selected fasta
>NDP-hexose C-4 aminotransferase [probable aminotransferase]
GTGATCAACCTGCACCAGCCGATCCTCGGCACCGAAGAACTCGACGCGATCGCGGAGGTG
TTCGCCTCCAACTGGATCGGGCTCGGGCCGCGCACCCGGACGTTCGAGGCCGAATTCGCC
CACCACCTGGGAGTGGATCCCGAACAGGTCGTGTTCCTCAACTCGGGGACTGCCGCGCTG
TTCCTTACCGTGCAGGTGCTCGACCTCGGCCCAGGCGACGACGTGGTACTTCCTTCGATA
AGCTTCGTGGCGGCGGCCAACGCCATCGCATCCTCCGGTGCCCGCCCGGTGTTCTGCGAC
GTCGACCCCCGGACGTTGAACCCCACGCTGGATGATGTGGCGAGGGCCATCACGCCGGCG
ACCAAGGCCGTATTGCTGCTCCACTATGGAGGATCGCCGGGAGAAGTCACCGCGATCGCC
GATTTCTGCCGTGAAAAGGGCCTCATGCTCATCGAGGACTCCGCCTGCGCGGTGGCATCG
TCCGTGCACGGCACCGCTTGCGGAACCTTTGGTGACCTGGCCACGTGGAGTTTCGATGCG
ATGAAGATCCTGGTCACCGGGGATGGGGGCATGTTCTACGCGGCGGATCCGGAGCTGGCG
CACCGCGCAAGACGACTCGCCTACCACGGTCTTGAGCAGATGAGCGGATTCGATTCGGCC
AAGTCTTCCAACCGCTGGTGGGATATTCGCGTCGAAGACATCGGCCAGCGGCTGATCGGG
AACGACATGACGGCAGCGCTTGGCAGCGTGCAGCTGCGCAAACTGCCAGAATTCATCAAC
AGGCGTAGAGAAATCGCTACGCAGTACGACCGGTTGCTTTCCGATGTGCCGGGTGTCCTC
CTACCGCCGACGCTACCGGATGGGCACGTCTCGTCACACTACTTCTACTGGGTCCAGCTG
GCTCCGGAGATCCGCGACCAGGTGGCGCAGCAAATGCTGGAACGCGGCATCTACACGAGC
TACCGCTACCCGCCCCTGCACAAGGTCCCCATCTACCGCGCGGACTGCAAGCTGCCTTCT
GCGGAGGACGCCTGCCGCAGAACACTCCTGCTACCACTGCACCCAAGCCTTGACGACGCC
GAGGTGCGCACGGTGGCTGACGAGTTCCAGAAGGCCGTCGAACACCACATCAGCCAAAGA
TCACCACTCCGAAAGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000653 DegT/DnrJ/EryC1/StrS aminotransferase (Family)
[1-377] 2.0000139277205e-83 PIRSF000390
PIRSF000390   PLP_StrS
[9-370] 1.10000000000001e-94 PF01041
PF01041   DegT_DnrJ_EryC1
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain)
[2-253] 9.49999999999983e-67 G3DSA:3.40.640.10
G3DSA:3.40.640.10   PyrdxlP-dep_Trfase_major_sub1
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain)
[255-375] 1.4e-30 G3DSA:3.90.1150.10
G3DSA:3.90.1150.10   PyrdxlP-dep_Trfase_major_sub2
IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain)
[1-376] 1.69998802370953e-90 SSF53383
SSF53383   PyrdxlP-dep_Trfase_major
SignalP
[1-34] 0.056 Signal
Eukaryota   
TMHMM No significant hit
Spino_00030 Spino_00030   Spino_00050 Spino_00050
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