Stigm_00100 Stigm_00100   Stigm_00120 Stigm_00120

Stigm_00110 : CDS information

close this sectionLocation

Organism
StrainSga15
Entry nameStigmatellin
Contig
Start / Stop / Direction26,284 / 31,941 / + [in whole cluster]
26,284 / 31,941 / + [in contig]
Location26284..31941 [in whole cluster]
26284..31941 [in contig]
TypeCDS
Length5,658 bp (1,885 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)StiC protein
Gene
Gene (GenBank)stiC
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[11809757] The biosynthesis of the aromatic myxobacterial electron transport inhibitor stigmatellin is directed by a novel type of modular polyketide synthase. (J Biol Chem. , 2002)
comment
S. aurantiacaにおいてstigmatellin形成を担う遺伝子セットの報告。

stiC (5659 bp)
KS-(17-443), AT-(559-977), DH-(888-1109), KR-(1474-1722),
ACP-(1740-1841)

以前に取得されたStigmatellinを合成できないMutantはこの遺伝子へのInsertionであったことが判明。
[13C]acetate and [13C]propionate取り込み実験で、polyketideを合成する基質が確認されている。

close this sectionPKS/NRPS Module

3 malonyl-CoA
KS17..394
AT556..875
DH925..1097
KR1474..1653
ACP1753..1829

close this sectionSequence

selected fasta
>polyketide synthase [StiC protein]
MQKMSTRLDALQRSKTEPIAIIGMGCRFPGGADTPEAFWRLLRDGVDATRQVPADRWDAD
ALYDADPDAPGKIYARRGGFLEHVDHFDAEFFGIAPREAKSIDPQQRLVAEVSWEALESA
GYASARLSGSATGVFIGAGIDDYAQFQLRSDDLEQIGPYAGTGTSLCFTAGRVSYLLGLQ
GPSLMVDTACSSSLVAVHLAMQSLRNGECRMALAGGVNLILSPEISIFLSRSHALAPDGR
CKTFDALADGYARGEGCGVIVLKRLSDAQQDKDRILAVIRGSAVNHDGPSSGLTVPNGLA
QQALLRQALSNARVEPARVGYVEAHGTGTPLGDPIELEALGTVLSEGRSREHPFALGSVK
TNVGHLESAAGVAGLIKVVLALQHREIPPHLHFERLNPRASLEGIPAVIPTRRIPWEAIG
GGRIAGVSSFGLSGTNAHVVLEEAPELVPPASTVAGTPSFERPLHLFALSAKSEQALRTA
AGNLSNHLVATESEAPHTLADACFSANVGRSHFGHRAVVVAETTATLRERLGKISSGTQV
PGVQEGQVAGAAPRVVFLFSGQGSQYIGMGRRLYETQPTFRSVLDRCDELIHPWLGRSLL
SVIYPESGATSPLDETLFTQPALFAIEYALAELWRSWGVTPSMVMGHSVGEYVAACVAGV
LRWEDGLQLIRERAQLMQTKTAPGEMAAVFADESEVLAAIAGHQERVSIAAYNGPSEIVI
SGARESIREVLGVLSARGVKSRKLNTSHAFHSPLMDPMLDEFERVASQMIHQEPRITLIS
NVTGRAAGAEVFSKPGYWRQHVRAPVRFFEGVRTLHERGATLFVEIGPNPVLMGLGQRIL
PEGAATFLPSLQKSRDDWSPMLESLGALYVRGVNVDWEGLDRDYSRRRTALPTYPFQRER
FWIDRGSRRSAREPVETSSREASPSVLLGRRVRSPALEQIVFESEFNTARLPFLSDHRIY
GEVVVPGACHLAMVISAGLELFGAGPLVLKDVVFPQPLIVPEGRSRWVQLILGTPCEPGT
PVSFRIVSAAEGEHTWVEHAVGGLLRERAVSPLEAATRDPSFEMGKTPDEEAVSALEVFY
RPFLEAGLALGPSFQWLTRIWKRDGQALSQMQAPEETRRAGGFPVHPGLIDACFQLFDSS
LPSAASVTVFVPVAVGCLRWSGSPGERPRCYVSVREASSASRERIVGDVRVVDATGTTAL
EMDAVSLRHAPKDALLRAVRPRLSDALYEVEWRHAAPRPSAQASGAPSGRWLVFAERKGF
GTALADRMGSRGVGCVLVSPGETYARLGDAHFAVNPKQPSDFVRLLEVLGASGEPPLRGI
VHAFGLGDRAASVSTETPSLTSLEARLELGCGSLLHLIQALAPSGHAASAKLWVVTAGAQ
PIQAGTGSVSSTHAALWGMARSLAAEHPELWGGLVDLDPANTNEVSATQLDSELGKSDRE
DQVAYRSESRHVARLRRRGASSGVGRPVALRSDGAYVLSGGMGGLGLQVAQWMVERGARR
LVLMGRGGPSEHASKSIREMERAGAQVLVVQGDVARPEDVAAALKVAGSEGWPIRGVVHA
AGVVQDGALLRQDWGRFTPVLAPKVAGAWNLHVQTLDQPLDFFVLFSSAASLLGPRGQGN
YAAGNAFLDALGHGRRSLGLPSLSINWGLWAGAGMGEGRSPSARRTLSTQGIEPIEPEEG
IRILEELLAGRAAQVAVLPIHWPTFVEQSLGGRSPAFIEELAGAIHSKPTAEASRTQVSD
FANQLAAASPSEQKELLTALVREEVGRVLGLEPSRPLKPQQRFFEAGMDSLMAIDVRRKL
QLRLGSAFELSSTLVFDYPTTQELAQYLVKQVSTAHSSAQRVDEPRAGETQSDAPSVVLD
VLSRNEVESRLDERLAAIEASMMDD
selected fasta
>polyketide synthase [StiC protein]
ATGCAGAAGATGAGCACCCGGCTCGATGCGCTGCAGCGCTCGAAGACAGAGCCCATTGCC
ATCATTGGAATGGGCTGTCGGTTTCCCGGCGGCGCCGACACTCCCGAGGCCTTCTGGCGC
CTCCTGAGGGATGGAGTCGACGCCACACGGCAGGTTCCCGCGGACCGGTGGGATGCGGAC
GCGCTGTACGATGCGGATCCCGATGCGCCCGGGAAGATCTATGCGCGGCGGGGCGGCTTC
CTCGAGCACGTCGATCACTTTGATGCCGAGTTCTTTGGGATTGCTCCCCGGGAAGCCAAG
TCCATCGATCCACAGCAACGGCTTGTGGCCGAGGTGAGCTGGGAAGCACTCGAGAGCGCG
GGGTATGCATCCGCTCGGCTTTCCGGCAGTGCGACCGGTGTCTTCATCGGCGCTGGCATC
GACGACTACGCTCAGTTCCAGCTTCGCTCCGACGATCTCGAGCAGATCGGCCCCTACGCG
GGGACGGGCACCAGCCTGTGCTTCACCGCCGGTCGCGTTTCCTACCTGCTGGGGCTCCAG
GGTCCGAGCCTCATGGTGGACACAGCCTGTTCGTCATCGCTCGTCGCGGTTCACCTCGCC
ATGCAGAGCCTGCGCAATGGCGAGTGCCGGATGGCTCTTGCGGGAGGGGTGAATCTCATC
CTCTCGCCCGAGATCTCGATCTTCCTCTCCAGGTCGCATGCACTTGCCCCCGACGGCCGG
TGCAAGACGTTCGACGCGTTGGCGGATGGTTACGCGCGCGGTGAAGGCTGCGGCGTGATC
GTACTCAAGCGGCTCTCCGACGCCCAGCAGGACAAGGACAGAATCCTCGCCGTCATCCGC
GGCTCGGCGGTCAACCACGATGGCCCGAGCAGTGGTTTGACCGTCCCCAATGGCTTGGCC
CAGCAAGCCCTTCTTCGGCAGGCTCTGTCGAATGCGCGGGTCGAGCCGGCGCGGGTGGGC
TACGTCGAAGCACACGGAACGGGGACACCGCTCGGTGATCCGATTGAGCTCGAGGCGTTG
GGGACGGTGCTCTCGGAGGGACGGAGCCGTGAGCATCCCTTCGCGCTCGGTTCGGTCAAG
ACGAATGTGGGACACCTCGAATCAGCCGCCGGAGTGGCCGGTTTGATCAAGGTCGTCTTG
GCGCTCCAACACCGGGAGATCCCACCGCACCTGCACTTCGAACGATTGAATCCGCGCGCC
TCGCTGGAGGGAATTCCCGCGGTGATTCCGACACGACGGATTCCATGGGAGGCGATCGGC
GGAGGGCGAATCGCAGGCGTGAGCTCGTTCGGCCTGAGTGGCACCAATGCCCATGTCGTG
CTCGAGGAGGCTCCGGAGCTGGTGCCTCCGGCCAGCACGGTGGCCGGGACCCCGTCGTTC
GAGCGGCCGCTCCATCTCTTCGCACTCTCAGCCAAGAGTGAGCAGGCACTTCGGACGGCG
GCAGGCAATCTCTCGAATCATCTGGTCGCCACGGAGTCGGAGGCTCCTCACACACTTGCC
GACGCTTGTTTCTCCGCGAACGTGGGGCGGTCGCATTTTGGCCATCGCGCCGTCGTGGTT
GCCGAGACCACGGCCACGCTCCGCGAGCGGCTCGGGAAGATTTCCTCCGGGACGCAGGTG
CCGGGCGTTCAAGAGGGGCAGGTTGCGGGCGCCGCGCCCCGAGTCGTCTTCTTGTTCTCG
GGGCAGGGCTCTCAATACATCGGAATGGGGCGTCGGCTTTACGAGACGCAGCCCACGTTC
CGGAGTGTGCTGGATCGCTGTGACGAGCTGATCCATCCCTGGCTCGGCCGCTCGCTCCTG
TCGGTGATCTACCCGGAGAGCGGGGCCACCTCGCCTCTGGACGAGACGCTCTTCACGCAG
CCCGCGCTCTTCGCGATCGAGTACGCCCTGGCAGAACTCTGGCGCTCCTGGGGCGTCACG
CCGTCCATGGTCATGGGCCACAGTGTGGGAGAGTACGTGGCGGCCTGTGTCGCGGGCGTG
CTTCGGTGGGAAGACGGCTTGCAGCTCATCCGCGAGCGCGCTCAGCTCATGCAAACGAAG
ACCGCGCCAGGCGAGATGGCGGCGGTGTTCGCTGATGAGTCGGAAGTCCTTGCCGCGATC
GCGGGGCATCAAGAGCGCGTCTCCATCGCGGCCTACAATGGTCCTTCCGAGATCGTGATC
TCCGGTGCGCGCGAGTCCATTCGAGAGGTGCTCGGTGTACTGTCAGCGCGCGGGGTGAAG
TCGCGAAAGCTGAACACCTCGCATGCGTTCCACTCGCCGCTCATGGATCCCATGCTCGAT
GAGTTCGAGCGAGTGGCCTCCCAGATGATTCATCAGGAGCCACGGATCACCCTGATCTCC
AACGTGACGGGGCGAGCCGCTGGGGCGGAAGTGTTTTCGAAGCCGGGCTACTGGCGTCAG
CATGTTCGCGCGCCGGTTCGTTTCTTCGAGGGAGTCCGGACGCTTCATGAGCGAGGTGCG
ACCCTCTTCGTGGAGATAGGCCCGAACCCCGTCCTGATGGGCTTGGGGCAGCGAATTCTT
CCGGAGGGGGCTGCGACGTTCCTGCCCTCACTCCAGAAAAGTCGGGACGACTGGTCGCCG
ATGCTCGAGAGCCTGGGTGCGCTCTATGTGCGCGGCGTGAACGTCGATTGGGAGGGCCTG
GATCGAGATTATTCGCGACGTAGGACGGCACTGCCCACCTATCCCTTTCAGAGGGAGCGG
TTCTGGATCGATCGAGGTTCGAGGAGGTCCGCGCGAGAGCCTGTCGAGACCTCTTCCAGG
GAGGCCTCGCCGTCAGTGCTCCTTGGGAGGCGGGTACGCTCTCCCGCACTCGAGCAGATC
GTGTTCGAGTCCGAGTTCAACACAGCGCGACTGCCGTTCCTTTCGGATCACCGCATCTAC
GGTGAAGTCGTCGTTCCCGGTGCCTGCCATCTGGCCATGGTCATTTCGGCGGGGCTCGAG
CTTTTCGGGGCAGGGCCCCTTGTCCTGAAGGACGTGGTCTTCCCCCAGCCGCTCATCGTG
CCTGAGGGCCGGTCCCGATGGGTCCAGCTTATTTTGGGCACGCCCTGTGAACCAGGGACT
CCCGTGTCGTTCCGGATCGTCAGCGCGGCGGAAGGAGAGCACACCTGGGTCGAGCATGCC
GTTGGAGGCTTGTTGCGCGAGCGTGCGGTGAGTCCGTTGGAGGCTGCCACGCGGGATCCC
TCGTTCGAGATGGGCAAGACGCCTGACGAAGAGGCGGTGTCGGCGCTCGAAGTCTTCTAT
CGGCCGTTCCTCGAGGCGGGCCTTGCGCTCGGGCCCTCATTCCAGTGGCTCACCCGAATC
TGGAAGCGTGACGGCCAAGCGCTGTCCCAGATGCAGGCGCCAGAGGAGACCCGGCGAGCC
GGTGGGTTCCCCGTGCACCCGGGGTTGATCGACGCCTGCTTCCAGCTCTTCGATTCGAGC
CTTCCCTCCGCCGCGAGCGTTACCGTCTTCGTCCCGGTCGCAGTGGGGTGCTTGAGGTGG
AGCGGATCGCCTGGAGAGCGCCCTCGCTGCTATGTCTCGGTGCGCGAAGCGTCGAGCGCC
AGCCGGGAGCGCATCGTTGGCGATGTGCGTGTCGTCGATGCAACCGGAACCACCGCGCTC
GAGATGGACGCTGTCTCGCTCCGGCACGCGCCCAAGGACGCGCTGTTGCGCGCGGTGCGC
CCGCGCCTCAGTGACGCGCTGTACGAAGTCGAATGGCGGCATGCCGCTCCGCGCCCTTCG
GCGCAGGCCAGCGGGGCCCCCTCGGGTCGGTGGTTGGTTTTCGCGGAGCGAAAGGGTTTC
GGCACGGCGCTGGCCGATCGGATGGGAAGTCGCGGAGTGGGGTGTGTGCTCGTGTCGCCC
GGTGAGACGTACGCGCGTCTCGGCGATGCTCATTTCGCAGTGAACCCGAAGCAGCCGTCT
GATTTCGTGCGCCTGCTCGAGGTGCTCGGCGCCTCGGGTGAACCTCCGTTGCGTGGGATC
GTCCATGCATTCGGCCTCGGGGATCGCGCGGCCTCCGTATCGACGGAAACCCCGTCGCTC
ACATCCCTCGAGGCACGCCTCGAGCTTGGATGCGGCAGTCTTCTCCATCTGATCCAGGCG
CTGGCTCCCTCCGGTCATGCCGCTTCAGCCAAGCTCTGGGTCGTGACCGCCGGAGCCCAG
CCGATTCAGGCAGGAACCGGCTCTGTGTCCAGCACGCACGCGGCATTGTGGGGAATGGCG
CGGAGCCTGGCCGCCGAGCATCCAGAACTCTGGGGAGGGCTCGTCGATTTGGATCCAGCG
AACACCAATGAGGTGAGCGCCACGCAGTTGGACAGCGAGTTGGGCAAGTCGGATCGAGAA
GACCAGGTGGCCTACCGTTCCGAATCCCGGCATGTCGCCCGGTTGCGTCGGCGCGGCGCA
TCCAGCGGGGTGGGGCGCCCGGTAGCCCTTCGCTCGGACGGCGCCTACGTGCTTTCAGGT
GGCATGGGAGGCCTCGGGTTGCAGGTGGCCCAGTGGATGGTCGAGCGCGGGGCTCGGCGT
CTCGTGCTGATGGGGCGTGGAGGCCCGTCAGAGCACGCGAGCAAGTCCATCCGGGAGATG
GAGCGGGCGGGTGCGCAGGTGCTCGTCGTTCAAGGAGACGTTGCGCGGCCAGAGGATGTT
GCCGCCGCGTTGAAGGTGGCTGGCTCCGAGGGGTGGCCGATTCGCGGTGTTGTCCACGCG
GCTGGCGTCGTCCAGGACGGTGCGCTCCTGCGCCAGGACTGGGGGCGATTTACGCCGGTG
TTGGCCCCCAAGGTGGCTGGGGCCTGGAACCTGCATGTGCAGACGCTGGATCAGCCGCTC
GACTTCTTCGTGCTCTTTTCCTCGGCCGCGTCGTTGTTGGGGCCCCGTGGCCAAGGTAAC
TATGCAGCCGGTAATGCCTTCCTGGACGCGTTGGGGCATGGCCGCCGCTCACTGGGATTG
CCCTCGCTGTCGATCAACTGGGGGCTGTGGGCGGGAGCAGGAATGGGAGAGGGTCGCAGT
CCCAGTGCCCGGCGGACCCTGAGCACCCAGGGGATCGAACCCATTGAACCTGAGGAGGGG
ATTCGCATCCTCGAAGAATTGCTTGCTGGGCGCGCGGCGCAGGTGGCCGTGCTTCCCATT
CACTGGCCCACCTTCGTGGAGCAATCGCTGGGAGGTCGATCTCCCGCCTTCATCGAAGAA
CTCGCTGGTGCCATACATTCCAAACCCACGGCTGAGGCATCGCGGACCCAGGTATCGGAT
TTCGCGAATCAGCTCGCTGCTGCTTCTCCGTCCGAGCAGAAAGAACTGCTGACGGCCCTC
GTTCGAGAAGAAGTGGGCCGGGTTCTTGGCCTGGAGCCGTCCCGCCCCCTGAAGCCGCAG
CAGCGCTTCTTCGAGGCGGGAATGGATTCGCTCATGGCGATCGATGTCCGGCGGAAATTG
CAGCTCAGATTGGGTTCAGCCTTCGAGTTATCCTCGACCCTGGTGTTTGATTACCCGACC
ACGCAAGAGCTTGCGCAATACCTTGTCAAACAGGTATCCACTGCTCATTCGTCGGCGCAG
CGGGTCGATGAGCCCCGTGCTGGCGAGACACAGAGCGACGCGCCCTCCGTTGTGCTCGAT
GTCCTGTCCCGGAACGAAGTGGAGTCCAGGCTGGATGAGAGATTGGCGGCCATTGAGGCC
TCGATGATGGATGACTGA
[3] KS17..394
[3] AT556..875
[3] malonyl-CoA748..752
[3] DH925..1097
[3] KR1474..1653
[3] ACP1753..1829
[3] KS49..1182
[3] AT1666..2625
[3] malonyl-CoA2242..2256
[3] DH2773..3291
[3] KR4420..4959
[3] ACP5257..5487

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[549-680] 1e-85 G3DSA:3.40.366.10 [748-864] 1e-85 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[1474-1640] 7.4e-55 PF00106
PF00106   adh_short
IPR006162 Phosphopantetheine attachment site (PTM)
[1785-1800] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1746-1832] 5.5e-15 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1761-1828] 3.7e-11 PF00550
PF00550   PP-binding
[1750-1850] 1.89999859865865e-17 SSF47336
SSF47336   ACP_like
[1753-1829] PS50075
PS50075   ACP_DOMAIN
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[17-268] 2.59999999999995e-100 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[276-394] 7.29999999999998e-45 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[556-875] 2.09999999999996e-70 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[554-858] 3.19999899046355e-76 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[682-747] 1.60000240695997e-17 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[17-279] 7.30000000000007e-102 G3DSA:3.40.47.10 [280-446] 5.69999999999998e-63 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[9-446] 2.7000136937899e-105 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1252-1469] 6e-19 G3DSA:3.40.50.720 [1475-1680] 9.09999999999992e-40 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[181-197] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[558-855] 9.80000272130214e-119 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1758-1832] 8.49999291746323e-21 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
[925-1097] 2.30000440726534e-42 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[19-446] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1474-1653] 1.79999754022375e-56 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit
Stigm_00100 Stigm_00100   Stigm_00120 Stigm_00120
Page top