Stigm_00120 Stigm_00120   Stigm_00140 Stigm_00140

Stigm_00130 : CDS information

close this sectionLocation

Organism
StrainSga15
Entry nameStigmatellin
Contig
Start / Stop / Direction37,740 / 43,553 / + [in whole cluster]
37,740 / 43,553 / + [in contig]
Location37740..43553 [in whole cluster]
37740..43553 [in contig]
TypeCDS
Length5,814 bp (1,937 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)StiE protein
Gene
Gene (GenBank)stiE
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[11809757] The biosynthesis of the aromatic myxobacterial electron transport inhibitor stigmatellin is directed by a novel type of modular polyketide synthase. (J Biol Chem. , 2002)
comment
S. aurantiacaにおいてstigmatellin形成を担う遺伝子セットの報告。

stiE (5814 bp)
KS-(34-460), AT-(568-886), OMT-(978-1252), KR-(1522-1769), ACP-(1785-1885)

[13C]acetate and [13C]propionate取り込み実験で、polyketideを合成する基質が確認されている。

close this sectionPKS/NRPS Module

5 methylmalonyl-CoA
KS35..411
AT565..881
OM981..1252
KR1522..1701
ACP1803..1872

close this sectionSequence

selected fasta
>polyketide synthase [StiE protein]
MSTGSQDHRSLLKQSFLEIERLQAKVDSLERAKSEPIAIVGVGCRFPGGSDDLASYWEFL
RDGVDGITEIPSERWDIDAFHDPNPEARWKMSTRRAGFIKDVDRFDASFFGISPREAASM
DPQQRVLLEVAWEALEDAGLPASRLGGSRTGVFMGVCSNDYTWLQLSEPERIDAPYFGIG
SAHNILAGRLSYIWDLRGPNIAVDTACSSSLVAVHLACQSLRAGECDAALAGGVSLILSP
LTLIFPSKMHMLAPDGRCKAFDAQADGFSRGEGCGVVVLKRLSDAQASGDEILAVIRGTS
INQDGRTNGLTAPNVLSQQVVIRKALEDAGMAPERIAFIESHGTGTNLGDPAELEALKAV
FGTRKAGEPSCAIGSVKSSVGHLEAAAGIAGLIKAALAVKHGAIPPNLHFQKINPNVNLE
GAPFVVPTGTQPWPESGAPRAAGVSAFGWSGTNAHVVLEEAPAKEARQESSQAARAQVLP
LSARGPEALRALAQRYREFLSRGESTGALPDICRAAAMKREQFDHRLAIVGEDSRAMVER
LDAFLQGEARSGLVSAVAGERRGIVFVFPGQGSQWVGMGRQLLEREPAFRTALEACDRAM
KPHVGWSAVEVLVGSEGAPRVDRIDVIQPILFAIEVALAATWRARGIEPDAVVGHSMGEV
AAAHVAGALSLDDAARIICRRSQLLAGLSGQGAMAVVELSQEEAERAIVGYEDRLSIAVC
NGPRSTVLSGDPQALTAVLATLESRGVFCRQVKVDVASHSPQMDPLRPALLNALQGLQPK
TAAVPIFSTVTGAIFDGTGMNAAYWVDNLRKPVLFSKAVSRLIEEGHALFIELSPHPILL
PPIDEQLRTLNRKGGVFPSMRREEPEQAVLVESLGALYTRGHPIDWSRSFPGEGRDVKLP
AYPFQRARYWNVASPAGLLPGAIVEGEGPRLDGSVADEGTSASEGTSGIIASFYDSLVTQ
AAGEDVRSSNDDEHFLTWGVFQEIVPGFSWIRTVFRPSERPEGRERLAVAQRELRRVLFR
AVDLSAIKNVMDFGCGHGSDLIILGEQNEHLKLDGYTISGKQAEVCKQRVRTRGLQNRIR
IFQRDSAKDDFPGMYDLVLGFEVAGLIPDKDALFSNIDRHLTNGGLLIMADFVANTLSPI
EVQETSTFSSTREQWNKLFSSNHLRLVDAVDVSNEVANCLHNPDYAAQFEALCKELKLDE
VTQRSFGSYENVYKALRGGLISYVLFHVQKDRFSRSDELFHLNAKQFEQLTPYAEFAKRR
DASGAQSDGDETKEWLYESQWRRETPPLTAQGPSASVGPGRWLILGDRGGVGETLMRRLE
ERGEKCTFALAGNSFQRVSERRYEIRPGQTEDFQRLVQEVFRSDSLPCKGVVHLWSLDGV
STQELTLDAIHAAQPLGVESALSVVQSIAWAGLRDAPRLWLVTAGAHVAGGETGPVAVAV
APLWGLGGTIVQEHPELRCSRVDLSGARRKEEIDALIETLWADGSEDQIALRGGERFVAR
LAHWTGERARRTDGEVAIRSDGTYLITGGYGGLGLTFARWLVDRGARHIILAGRNGAPES
ARGAIEELRKAGAEIIAAKMDVADAEQTARVLEEARKTLPPLRGVIHSAVVLDDGVLLKQ
NRQRLERAMAPKVDGTWNLHRLTLDCPLDFFLLFSSLASMLGAPGQGNYTAAGAFQDAFV
FYRRALGLPAMTINWSPWSDVGQAVVQANRGERLAQGGLQSINPEQGVRVLERLLQHTAP
QIGVMRLDLRQWRQFYPKNASSPLFAELMRALNTAKPTKVASDSSVRVALQSLEPGPRRR
EMLEEHLQTLAAQVLRMSPKDLDRSTPLRSLGFDSLVAVEFRNRIEASLGLTLSATLAWN
HPTIEAMTTHIADKMGLVLSVAEAPSQAAEQSGSRAAGDLEALAEGLLDELEGLSDDESG
RLASGTAQRSPREDINE
selected fasta
>polyketide synthase [StiE protein]
ATGAGCACTGGAAGCCAGGACCACCGAAGTCTCCTCAAGCAGTCGTTCCTGGAGATCGAG
CGGCTCCAAGCCAAGGTCGATTCGCTCGAGCGAGCCAAGTCGGAGCCTATTGCCATTGTG
GGAGTGGGTTGCCGCTTCCCAGGGGGCAGTGACGATCTGGCTTCGTACTGGGAGTTCCTC
CGCGACGGTGTGGACGGCATCACGGAGATTCCGTCCGAGCGCTGGGACATCGATGCATTC
CATGATCCCAATCCCGAAGCGCGCTGGAAGATGAGCACCCGTCGCGCCGGCTTCATCAAG
GACGTGGACCGCTTCGATGCCTCGTTCTTTGGCATCTCGCCTCGGGAAGCCGCGAGCATG
GACCCTCAGCAGCGCGTGCTCCTGGAGGTCGCCTGGGAGGCGCTCGAGGACGCCGGATTG
CCCGCGAGTCGGCTGGGAGGGAGCCGGACGGGCGTCTTCATGGGGGTTTGCAGCAACGAT
TACACATGGCTCCAGCTCAGTGAGCCTGAGCGCATTGACGCGCCGTACTTTGGTATCGGC
TCGGCCCACAACATCCTGGCTGGACGTCTGTCCTATATCTGGGATCTCCGAGGTCCGAAC
ATCGCGGTCGACACGGCGTGTTCCTCGTCGCTCGTGGCGGTCCACCTGGCCTGTCAGAGC
CTGCGCGCGGGAGAGTGCGATGCGGCGCTCGCGGGTGGGGTCAGTCTCATCCTCTCGCCG
TTGACCCTCATCTTCCCGAGCAAGATGCACATGCTCGCCCCCGATGGTCGGTGCAAGGCG
TTCGACGCTCAGGCCGATGGCTTCAGCCGCGGCGAGGGGTGCGGCGTCGTCGTCTTGAAG
AGGCTGTCGGATGCGCAAGCGAGTGGTGATGAGATCCTCGCGGTCATCCGCGGGACGAGC
ATCAACCAGGACGGTCGCACCAATGGGCTCACCGCGCCGAACGTGCTCTCGCAGCAGGTG
GTCATCCGGAAGGCCCTGGAGGACGCGGGGATGGCACCGGAGCGGATTGCTTTCATCGAG
TCTCATGGAACTGGGACCAACTTGGGAGATCCCGCGGAGCTCGAGGCGCTGAAGGCCGTC
TTCGGAACGCGGAAGGCAGGCGAGCCGAGCTGTGCCATTGGTTCGGTGAAGAGCAGCGTC
GGCCATCTTGAGGCCGCCGCTGGGATCGCGGGCCTCATCAAGGCTGCCCTCGCGGTGAAG
CACGGCGCCATTCCTCCGAACCTGCATTTCCAGAAGATCAACCCGAACGTGAACCTTGAA
GGGGCACCGTTTGTCGTTCCCACCGGGACGCAGCCGTGGCCCGAGAGTGGGGCCCCGCGC
GCCGCGGGCGTGAGCGCTTTCGGTTGGTCTGGAACCAATGCCCATGTGGTGCTGGAGGAG
GCCCCAGCTAAGGAAGCGCGGCAGGAATCCTCCCAGGCTGCGCGTGCGCAGGTTCTGCCC
CTTTCAGCCCGGGGACCTGAGGCGCTTCGTGCGCTCGCACAGCGTTACCGTGAATTCCTG
TCGAGGGGTGAGAGCACGGGGGCTCTGCCCGACATCTGCCGTGCCGCGGCGATGAAGCGG
GAGCAGTTCGATCATCGGCTCGCCATTGTTGGTGAGGACTCCCGGGCCATGGTCGAGCGG
CTCGATGCCTTCCTGCAGGGGGAGGCGCGCTCTGGCCTGGTTTCGGCGGTCGCAGGTGAG
CGCCGAGGCATTGTTTTCGTCTTCCCAGGGCAGGGCTCCCAGTGGGTGGGGATGGGCCGT
CAGCTCCTGGAGCGCGAGCCCGCCTTCCGGACTGCGCTAGAGGCCTGTGATCGGGCGATG
AAGCCGCATGTGGGCTGGTCGGCGGTCGAAGTGCTGGTGGGTAGCGAAGGGGCGCCACGC
GTTGATCGGATCGATGTCATCCAGCCGATCCTGTTCGCGATCGAGGTCGCCTTGGCCGCG
ACGTGGCGCGCCCGTGGAATCGAGCCTGACGCGGTGGTGGGACACAGCATGGGCGAGGTG
GCCGCCGCGCACGTCGCGGGTGCCCTGAGCCTCGACGATGCCGCTCGCATCATCTGCCGT
CGCAGTCAACTGCTCGCGGGGCTGAGCGGGCAGGGTGCGATGGCCGTCGTCGAACTCTCC
CAGGAGGAAGCGGAGCGGGCGATCGTGGGCTACGAGGATCGCCTGTCGATCGCCGTCTGC
AATGGCCCACGCTCGACGGTGCTCTCGGGCGATCCTCAAGCGCTCACGGCCGTGCTCGCC
ACGCTGGAGTCGCGAGGGGTGTTCTGCCGCCAGGTCAAGGTCGACGTCGCGTCCCACAGC
CCGCAGATGGATCCGCTGCGCCCTGCGCTGCTCAACGCGCTCCAGGGACTCCAGCCGAAG
ACCGCCGCCGTGCCGATCTTCTCGACCGTGACGGGAGCGATCTTCGATGGCACTGGGATG
AACGCCGCCTATTGGGTCGACAACCTGCGAAAGCCGGTTCTGTTCTCCAAGGCCGTGAGT
CGGCTTATTGAAGAAGGCCACGCCTTGTTCATCGAGCTCAGTCCACACCCCATCCTGTTG
CCGCCCATCGATGAGCAACTCCGCACGCTGAATCGCAAGGGTGGCGTCTTTCCGTCGATG
CGTCGTGAGGAACCGGAGCAGGCGGTGCTGGTGGAATCCCTGGGTGCCCTTTACACCCGT
GGCCATCCGATCGACTGGAGCCGCTCGTTCCCCGGTGAGGGCCGCGACGTCAAGCTGCCG
GCCTATCCCTTCCAGCGGGCGCGTTACTGGAATGTCGCCAGTCCAGCGGGGCTGCTGCCT
GGGGCCATTGTGGAAGGTGAAGGGCCGCGTCTGGATGGCTCGGTAGCGGATGAGGGGACG
AGCGCGTCAGAGGGGACGAGCGGCATTATCGCCTCCTTCTATGATTCCCTCGTCACGCAG
GCCGCCGGCGAAGACGTCCGGAGCTCGAACGACGACGAGCACTTTCTCACCTGGGGCGTC
TTCCAAGAGATTGTCCCTGGGTTCTCTTGGATACGGACCGTGTTCAGGCCCAGCGAGCGT
CCTGAGGGGCGCGAGCGGCTGGCCGTGGCGCAGCGTGAACTCCGACGGGTTCTGTTTCGA
GCGGTCGATCTTTCCGCCATCAAGAACGTGATGGACTTCGGCTGCGGTCACGGCTCGGAC
CTGATCATCCTCGGTGAGCAGAATGAGCACCTCAAGCTCGATGGCTATACGATCTCGGGC
AAGCAAGCGGAGGTGTGCAAGCAGCGCGTGCGCACACGGGGGTTGCAGAACCGCATCCGG
ATCTTCCAGCGGGACAGCGCCAAGGATGATTTTCCGGGCATGTACGACCTCGTCCTCGGC
TTCGAGGTCGCCGGGCTCATTCCCGACAAGGATGCGCTGTTCTCGAACATCGACAGGCAC
CTCACCAACGGCGGTCTGCTCATCATGGCGGATTTCGTCGCCAATACGCTGTCTCCCATC
GAGGTTCAGGAGACGTCGACGTTCTCCAGCACGCGGGAGCAGTGGAACAAGCTCTTCTCG
TCCAATCACCTTCGGCTCGTCGACGCTGTGGACGTGAGCAACGAGGTCGCCAACTGCCTG
CACAACCCGGATTACGCGGCGCAGTTCGAGGCGCTCTGCAAGGAGTTGAAGCTCGACGAG
GTGACGCAGCGGAGCTTCGGCTCGTACGAGAACGTCTACAAGGCGCTTCGAGGGGGCCTC
ATCAGCTACGTCCTGTTCCACGTCCAGAAGGATCGGTTCAGCCGGTCCGATGAACTCTTC
CACCTCAATGCGAAGCAGTTCGAGCAGTTGACGCCGTACGCGGAGTTCGCCAAGCGGCGG
GATGCGTCCGGCGCGCAGTCGGATGGCGATGAAACCAAGGAGTGGCTGTACGAGAGCCAG
TGGCGCCGGGAGACGCCGCCGCTCACTGCCCAGGGTCCGAGCGCCTCTGTTGGACCGGGG
CGCTGGCTGATTCTCGGAGACCGGGGGGGCGTCGGCGAGACCTTGATGCGCCGCCTGGAG
GAGCGAGGCGAGAAGTGTACCTTCGCGCTCGCAGGGAACTCCTTCCAGCGCGTGTCCGAG
CGTCGTTATGAGATTCGCCCCGGGCAGACAGAGGACTTCCAGCGCCTCGTTCAGGAGGTG
TTCCGCTCCGATTCCCTGCCTTGTAAGGGGGTCGTGCACCTCTGGTCGCTCGATGGTGTC
TCTACCCAGGAACTGACCCTCGATGCGATTCACGCCGCGCAGCCGCTCGGAGTCGAGAGC
GCGCTCTCCGTTGTTCAATCGATCGCCTGGGCGGGCCTGCGCGACGCGCCCCGGTTGTGG
CTCGTGACCGCTGGAGCGCACGTGGCTGGAGGCGAGACGGGCCCCGTGGCGGTCGCGGTC
GCTCCCCTCTGGGGCTTGGGTGGAACCATCGTGCAAGAGCACCCAGAGCTGCGTTGCTCA
CGGGTCGATCTGAGCGGTGCACGGCGGAAAGAAGAGATCGACGCGCTCATCGAGACACTG
TGGGCGGATGGCTCCGAGGATCAGATCGCCCTGCGGGGAGGCGAGCGCTTCGTGGCGCGA
CTCGCGCACTGGACCGGTGAGCGCGCGCGGCGAACGGACGGCGAGGTGGCCATTCGTTCG
GATGGTACCTACCTGATCACCGGTGGATACGGTGGTCTCGGGTTGACCTTCGCCCGCTGG
CTGGTTGATCGCGGTGCCCGCCACATCATTCTGGCAGGCCGGAACGGAGCGCCCGAATCC
GCGCGCGGGGCGATCGAGGAATTGCGGAAGGCCGGTGCTGAGATCATCGCCGCGAAGATG
GACGTCGCGGATGCGGAGCAAACCGCTCGCGTGCTCGAGGAGGCACGCAAGACCCTGCCT
CCCTTGCGAGGCGTCATCCACAGCGCCGTGGTGCTCGATGATGGTGTTTTGCTCAAGCAG
AACCGCCAGCGTCTCGAGCGCGCGATGGCGCCGAAGGTCGATGGCACCTGGAACCTGCAC
CGGCTCACGCTCGATTGCCCCCTCGACTTCTTCCTGCTGTTCTCGTCGTTGGCCTCGATG
CTGGGGGCACCTGGGCAGGGCAACTACACGGCGGCCGGAGCGTTCCAGGATGCGTTCGTG
TTCTACCGGCGAGCTCTTGGACTGCCCGCGATGACCATCAACTGGTCTCCGTGGAGTGAC
GTGGGCCAGGCGGTCGTTCAGGCCAATCGCGGCGAGCGGCTCGCGCAGGGTGGGCTCCAG
AGCATCAACCCGGAGCAAGGCGTGCGCGTCCTTGAGCGGCTGCTTCAGCACACGGCCCCG
CAGATCGGCGTGATGCGGCTCGATTTGCGGCAGTGGCGACAGTTCTATCCGAAGAACGCC
AGCTCGCCGCTGTTCGCCGAGCTCATGCGTGCGCTGAATACCGCCAAGCCGACCAAGGTG
GCGTCGGATTCGTCGGTTCGCGTGGCACTCCAGTCCCTCGAGCCTGGGCCCCGGCGCCGG
GAGATGCTCGAAGAGCACCTCCAGACACTGGCGGCCCAGGTCCTGAGGATGTCGCCGAAA
GATCTCGACCGGAGCACTCCGCTACGCAGCTTGGGTTTCGACTCGCTCGTCGCGGTCGAA
TTCCGTAACCGCATCGAAGCCAGTCTGGGGCTCACACTCTCCGCAACACTGGCCTGGAAT
CACCCGACCATCGAGGCGATGACGACCCATATCGCAGACAAGATGGGGCTCGTCTTGAGT
GTGGCCGAGGCTCCCTCTCAGGCGGCGGAGCAGTCGGGCTCACGCGCCGCTGGGGATCTC
GAGGCCCTGGCCGAAGGGCTCCTCGACGAGTTGGAAGGGCTGTCGGATGACGAGAGTGGC
CGGTTGGCGAGCGGCACGGCCCAGCGAAGCCCGCGGGAGGACATCAATGAGTAG
[5] KS35..411
[5] AT565..881
[5] methylmalonyl-CoA756..760
[5] OM981..1252
[5] KR1522..1701
[5] ACP1803..1872
[5] KS103..1233
[5] AT1693..2643
[5] methylmalonyl-CoA2266..2280
[5] OM2941..3756
[5] KR4564..5103
[5] ACP5407..5616

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[557-688] 1.99999999999999e-74 G3DSA:3.40.366.10 [756-872] 1.99999999999999e-74 G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[1522-1688] 1.80000000000002e-57 PF00106
PF00106   adh_short
IPR009081 Acyl carrier protein-like (Domain)
[1807-1871] 6.50000000000001e-12 PF00550
PF00550   PP-binding
[1803-1872] PS50075
PS50075   ACP_DOMAIN
[1800-1875] 9.9e-19 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1796-1909] 3.60000433965927e-20 SSF47336
SSF47336   ACP_like
IPR013217 Methyltransferase type 12 (Domain)
[1031-1127] 2.2e-10 PF08242
PF08242   Methyltransf_12
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[35-285] 7.5e-99 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[293-411] 4.5e-44 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[565-881] 2.90000000000003e-99 PF00698
PF00698   Acyl_transf_1
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[563-852] 2.99998750445706e-69 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[690-755] 9.00000407957274e-18 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[34-296] 5.80000000000004e-104 G3DSA:3.40.47.10 [297-464] 3.99999999999998e-64 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[26-409] 9.30000048475962e-104 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1521-1736] 2.19999999999997e-40 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[198-214] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[567-864] 2.2999842048857e-125 SM00827
SM00827   PKS_AT
IPR020803 Polyketide synthase, methyltransferase domain (Domain)
[981-1252] SM00828
SM00828   PKS_MT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1804-1875] 2.70000183580794e-25 SM00823
SM00823   PKS_PP
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[37-463] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1522-1701] 1.10000150671643e-63 SM00822
SM00822   PKS_KR
SignalP No significant hit
TMHMM No significant hit
Stigm_00120 Stigm_00120   Stigm_00140 Stigm_00140
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