Vicen_00020 Vicen_00020   Vicen_00040 Vicen_00040

Vicen_00030 : CDS information

close this sectionLocation

Organism
StrainHC34
Entry nameVicenistatin
Contig
Start / Stop / Direction1,759 / 2,730 / + [in whole cluster]
1,759 / 2,730 / + [in contig]
Location1759..2730 [in whole cluster]
1759..2730 [in contig]
TypeCDS
Length972 bp (323 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative dTDP-glucose 4,6-dehydratase
Product (GenBank)dTDP-glucose 4,6-dehydratase
Gene
Gene (GenBank)vinB
EC number4.2.1.46
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[15112997] Cloning, sequencing, and functional analysis of the biosynthetic gene cluster of macrolactam antibiotic vicenistatin in Streptomyces halstedii. (Chem Biol. , 2004)
comment
vicenistatin生合成gene clusterの同定論文。

VinB (323aa) : dTDP-glucose 4,6-dehydratase

配列解析からの機能推定。
vicenistatinに含まれるアミノ糖vicenisamineの生合成第二ステップに関与。
dTDP glucose->dTDP-4-keto-6-deoxyglucose
Related Reference
ACC
Q194R3
NITE
Mith_00070
PmId
[9150235] Cloning and insertional inactivation of Streptomyces argillaceus genes involved in the earliest steps of biosynthesis of the sugar moieties of the antitumor polyketide mithramycin. (J Bacteriol. , 1997)
comment
11th, 72%, 1e-124
Streptomyces argillaceus_mtmE
dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)

E. coli発現タンパクで活性みている。
mtmD: glucose-1-phosphate:dTTP thymidylyl transferase
mtmE: dTDP-D-glucose 4,6-dehydratase

close this sectionSequence

selected fasta
>putative dTDP-glucose 4,6-dehydratase [dTDP-glucose 4,6-dehydratase]
MSTRLLVTGAAGFIGSAYVRGLLTDQPDLRVTVLDSLTYAGNRANLDLTHPSLDLVEGDI
CDTELVDRLTAEADQIVHFAAESHVDRSITGSAEFIRTNVLGTHTLLDAALRHGIDRFVH
ISTDEVYGSIEKGSWPETDPLRPNSPYSASKASSDLLALAYHRTHGLDVRVTRCSNNYGP
YQHPEKVIPLFVTNLLDGKRVPLYGDGQNVRDWLHVEDHCAAIECVRTRGGAGEIYNIGG
GTELSNRELTGLLLEACGADWDSVEYVTDRKGHDLRYSVDWSKVADLGYTPAHDFRAGLA
ETVDWYRSNRTWWEPAKYGHSPA
selected fasta
>putative dTDP-glucose 4,6-dehydratase [dTDP-glucose 4,6-dehydratase]
ATGAGCACCCGACTGCTCGTCACGGGCGCCGCCGGATTCATCGGATCGGCCTACGTCCGC
GGACTCCTCACCGACCAGCCCGACCTGCGGGTCACCGTCCTCGACAGCCTCACCTACGCG
GGCAACCGGGCCAACCTCGACCTGACACACCCCTCCCTCGACCTCGTCGAGGGTGACATC
TGCGACACCGAGCTCGTGGACCGCCTCACCGCCGAAGCCGACCAGATCGTCCACTTCGCC
GCCGAGAGCCACGTCGACCGGTCCATCACCGGCTCCGCCGAGTTCATCCGCACCAACGTC
CTCGGCACCCACACCCTCCTCGACGCCGCGCTCCGCCACGGCATCGACCGGTTCGTCCAC
ATCTCCACGGACGAGGTCTACGGATCGATCGAGAAGGGCTCCTGGCCGGAGACCGACCCG
CTGCGCCCCAACTCCCCCTACTCGGCGTCCAAGGCATCCAGCGACCTCCTGGCGCTCGCC
TACCACCGGACCCACGGGCTCGACGTACGGGTCACCCGTTGCTCCAACAACTATGGCCCC
TACCAGCATCCCGAGAAGGTCATCCCGCTCTTCGTCACCAACCTCCTCGACGGCAAGCGG
GTACCCCTGTACGGGGACGGCCAGAACGTCCGCGACTGGCTGCACGTCGAGGACCACTGC
GCCGCCATCGAATGCGTCCGCACCCGCGGTGGCGCCGGGGAGATCTACAACATCGGCGGC
GGCACCGAACTGAGCAACCGGGAACTCACCGGCCTGCTCCTGGAGGCGTGCGGGGCGGAC
TGGGACAGCGTGGAGTACGTCACCGACCGCAAGGGCCACGACCTGCGCTACTCCGTCGAC
TGGTCCAAGGTCGCCGACCTCGGCTACACCCCCGCCCACGACTTCCGTGCCGGGCTCGCG
GAGACCGTCGACTGGTACCGCTCCAACCGCACCTGGTGGGAACCGGCGAAGTACGGCCAC
AGCCCCGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
[6-239] 1.29999999999998e-69 PF01370
PF01370   Epimerase
IPR005888 dTDP-glucose 4,6-dehydratase (Family)
[4-315] TIGR01181
TIGR01181   DTDP_gluc_dehyt
IPR016040 NAD(P)-binding domain (Domain)
[4-195] 3.19999999999996e-66 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-19] 0.927 Signal
Bacteria, Gram-negative   
[1-21] 0.953 Signal
Eukaryota   
TMHMM No significant hit
Vicen_00020 Vicen_00020   Vicen_00040 Vicen_00040
Page top