Vicen_00070 Vicen_00070   Vicen_00090 Vicen_00090

Vicen_00080 : CDS information

close this sectionLocation

Organism
StrainHC34
Entry nameVicenistatin
Contig
Start / Stop / Direction33,972 / 33,262 / - [in whole cluster]
33,972 / 33,262 / - [in contig]
Locationcomplement(33262..33972) [in whole cluster]
complement(33262..33972) [in contig]
TypeCDS
Length711 bp (236 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose N-methyltransferase
Product (GenBank)N-methyltransferase
Gene
Gene (GenBank)vinG
EC number2.1.1.-
Keyword
  • vicenisamine
Note
Note (GenBank)
Reference
ACC
PmId
[15112997] Cloning, sequencing, and functional analysis of the biosynthetic gene cluster of macrolactam antibiotic vicenistatin in Streptomyces halstedii. (Chem Biol. , 2004)
comment
vicenistatin生合成gene clusterの同定論文。

VinG (236aa) : N-methyltransferase

配列解析からの機能推定。
vicenistatinに含まれるアミノ糖vicenisamineの生合成最終ステップ。
amino基をN-methyl化して、dTDP-vicenisamineを得ると考えられる。
Related Reference
ACC
Q9ZGH6
NITE
Pikro_00100
PmId
[9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998)
[12119032] Expression, purification, and characterization of two N,N-dimethyltransferases, tylM1 and desVI, involved in the biosynthesis of mycaminose and desosamine. (Biochemistry. , 2002)
comment
15th, 45%, 1e-44
Streptomyces venezuelae_desVI
N,N-dimethyltransferase

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[PMID: 9770448](1998)
12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。

DesVI: N,N-Dimethyltransferase

desI, desII, desIII, desIV, desV, desVI, and desVIII はdeoxysugar生合成を担う。

desVI のDeletion/replacement mutationにより、methymycin, neomethymycin, narbomycin, and pikromycinは非産生。それらのaglyconeである10-deoxymethynolide and narbonolideを蓄積。

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[PMID: 12119032](2002)abstract
N,N-dimethyltransferasesであるtylM1 and desVI の過剰発現、精製、触媒機能確認。
TylM1 and DesVI はhomodimeric proteinで、ほとんど同じ生化学的特性を持つ。

close this sectionSequence

selected fasta
>putative NDP-hexose N-methyltransferase [N-methyltransferase]
MYEEDFARVYDDIYRRHKDYAGEAARIRELALERNPGAADLLDVGCGTGEHLALLRDDFA
VTGVDLSAPMVRVAEAKLPGVPVHEGDMRTFGLDRSFDVICSMYSSVGYLETLDGLFAAL
KNMAHHLRPGGVLIIEPWILRDSWNGGDLVEASFENEGGKVVRMGRWTTRAGRSHVEMHY
LVTSGDDPVRHFVDEQELSLFSKDEYEEAFRAAGCSVEYLPDGYVDRGVFVGVRQP
selected fasta
>putative NDP-hexose N-methyltransferase [N-methyltransferase]
ATGTACGAAGAGGACTTCGCGCGCGTCTATGACGACATCTATCGGCGGCACAAGGACTAC
GCCGGTGAGGCCGCCCGCATCCGCGAGCTGGCGCTCGAACGCAACCCCGGAGCGGCCGAT
CTGCTCGATGTGGGCTGTGGCACCGGCGAGCACCTCGCGCTGCTGAGGGACGATTTCGCC
GTCACCGGTGTGGACCTGTCGGCGCCGATGGTCCGGGTCGCCGAGGCCAAGCTGCCGGGG
GTGCCGGTCCACGAGGGCGATATGCGCACCTTCGGGCTCGACCGGAGCTTCGACGTGATC
TGTTCGATGTACAGCTCCGTCGGCTATCTGGAGACCCTGGACGGTCTGTTCGCCGCCCTG
AAGAACATGGCCCACCATCTGCGTCCCGGCGGGGTGCTGATCATCGAGCCGTGGATTCTG
CGGGACAGTTGGAACGGCGGCGATCTGGTGGAGGCGAGCTTCGAGAACGAGGGCGGCAAG
GTGGTCCGCATGGGCCGCTGGACCACCCGGGCCGGGCGCAGCCATGTGGAGATGCACTAC
CTGGTGACGAGCGGCGACGATCCCGTACGGCACTTCGTGGACGAACAGGAGCTGTCGCTG
TTCTCCAAGGACGAGTACGAGGAGGCGTTCCGCGCGGCCGGGTGCTCGGTGGAGTACCTG
CCCGACGGGTATGTCGACCGCGGTGTCTTCGTGGGGGTGCGACAGCCATAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP No significant hit
TMHMM No significant hit
Vicen_00070 Vicen_00070   Vicen_00090 Vicen_00090
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