Pikro_00010 : CDS information

Location

Organism	Streptomyces venezuelae
Strain	ATCC 15439
Entry name	Pikromycin
Contig	AF079138
Start / Stop / Direction	154 / 1,122 / + [in whole cluster] 154 / 1,122 / + [in contig]
Location	154..1122 [in whole cluster] 154..1122 [in contig]
Type	CDS
Length	969 bp (322 aa)

Annotation

Category	4.4 resistance
Product	23S rRNA adenine N-6-methyltransferase
Product (GenBank)	rRNA methyltransferase PikR2
Gene
Gene (GenBank)	pikR2
EC number	2.1.1.-
Keyword
Note
Note (GenBank)	mls type resistance protein
Reference	ACC Q9ZGI7 PmId [9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998) [26438831] Resistance to ketolide antibiotics by coordinated expression of rRNA methyltransferases in a bacterial producer of natural ketolides. (Proc Natl Acad Sci U S A. , 2015) comment [PMID: 9770448](1998) 12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。 PikR2: rRNA methyltransferase (mls resistance) pikR1 and pikR2はmacrolide-lincosamide-streptogramin B type resistant genesであり、たぶん細胞の自己防衛を供給する。 --- [PMID: 26438831](2015) PikR1 and PikR2 enzymesはそれぞれ、23S rRNA nucleotide A2058におけるN6アミノ基をmono- and dimethylateすることを確認。
Related Reference	ACC Q9R622 PmId [1328813] Molecular cloning and characterization of two lincomycin-resistance genes, lmrA and lmrB, from Streptomyces lincolnensis 78-11. (Mol Microbiol. , 1992) comment 3rd(Q9R622) 58%, 2e-72 Streptomyces lincolnensis Lincomycin-resistance determinant LMRB =23S RRNA ADENINE(2058)-N- methyltransferase homolog lincomycin-resistance決定因子lmrA and lmrBのクローニング。 lmrBはmacrolide-lincosamide-streptogramin(MLS) resistanceを与える23S rRNA adenine(2058)-N-methyltransferasesに有意に似たタンパクをコードする。 LmrA and LmrBの両酵素はlincomycinに限った基質特異性を持ち、celesticetin and clindamycinの様なその他のlincosamidesやmacrolidesへの抵抗性を引き起こさなかった。
Related Reference	ACC P07287 PmId [7961464] Domain V of 23S rRNA contains all the structural elements necessary for recognition by the ErmE methyltransferase. (J Bacteriol. , 1994) [7793855] Erythromycin resistance by ribosome modification. (Antimicrob Agents Chemother. , 1995) comment 44th(P07287) 33%, 6e-30 Saccharopolyspora erythraea (strain NRRL 23338)_ermE rRNA adenine N-6-methyltransferase(EC 2.1.1.48) 抗生物質抵抗性、23S rRNA modificationによるmethylTF発現測定。 virtoでのmethylation assay.

Sequence

>23S rRNA adenine N-6-methyltransferase [rRNA methyltransferase PikR2]
MAFSPQGGRHELGQNFLVDRSVIDEIDGLVARTKGPILEIGPGDGALTLPLSRHGRPITA
VELDGRRAQRLGARTPGHVTVVHHDFLQYPLPRNPHVVVGNVPFHLTTAIMRRLLDAQHW
HTAVLLVQWEVARRRAGVGGSTLLTAGWAPWYEFDLHSRVPARAFRPMPGVDGGVLAIRR
RSAPLVGQVKTYQDFVRQVFTGKGNGLKEILRRTGRISQRDLATWLRRNEISPHALPKDL
KPGQWASLWELTGGTADGSFDGTAGGGAAGSHGAARVGAGHPGGRVSASRRGVPQARRGR
GHAVRSSTGTEPRWGRGRAESA

>23S rRNA adenine N-6-methyltransferase [rRNA methyltransferase PikR2]
ATGGCATTTTCCCCGCAGGGCGGCCGACACGAGCTCGGTCAGAACTTCCTCGTCGACCGG
TCAGTGATCGACGAGATCGACGGCCTGGTGGCCAGGACCAAGGGTCCGATACTGGAGATC
GGTCCGGGTGACGGCGCCCTGACCCTGCCGCTGAGCAGGCACGGCAGGCCGATCACCGCC
GTCGAGCTCGACGGCCGGCGCGCGCAGCGCCTCGGTGCCCGCACCCCCGGTCATGTGACC
GTGGTGCACCACGACTTCCTGCAGTACCCGCTGCCGCGCAACCCGCATGTGGTCGTCGGC
AACGTCCCCTTCCATCTGACGACGGCGATCATGCGGCGGCTGCTCGACGCCCAGCACTGG
CACACCGCCGTCCTCCTCGTCCAGTGGGAGGTCGCCCGGCGCCGGGCCGGCGTCGGCGGG
TCGACGCTGCTGACGGCCGGCTGGGCGCCCTGGTACGAGTTCGACCTGCACTCCCGGGTC
CCCGCGCGGGCCTTCCGTCCGATGCCGGGCGTGGACGGAGGAGTACTGGCCATCCGGCGG
CGGTCCGCGCCGCTCGTGGGCCAGGTGAAGACGTACCAGGACTTCGTACGCCAGGTGTTC
ACCGGCAAGGGGAACGGGCTGAAGGAGATCCTGCGGCGGACCGGGCGGATCTCGCAGCGG
GACCTGGCGACCTGGCTGCGGAGGAACGAGATCTCGCCGCACGCGCTGCCCAAGGACCTG
AAGCCCGGGCAGTGGGCGTCGCTGTGGGAGCTGACCGGCGGCACGGCCGACGGATCCTTC
GACGGTACGGCGGGCGGTGGCGCGGCCGGATCGCACGGGGCGGCTCGGGTCGGGGCCGGT
CACCCGGGCGGCCGGGTGTCCGCGAGCCGGCGGGGCGTGCCGCAGGCGCGGCGCGGCCGG
GGGCATGCGGTACGGAGCTCCACGGGGACCGAGCCGAGGTGGGGCAGGGGGCGGGCGGAG
AGCGCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001737 Ribosomal RNA adenine methylase transferase (Family) PF00398 RrnaAD IPR020596 Ribosomal RNA adenine methylase transferase, conserved site (Conserved_site) PS01131 RRNA_A_DIMETH IPR020598 Ribosomal RNA adenine methylase transferase, N-terminal (Domain) SM00650 rADc
SignalP	No significant hit
TMHMM	No significant hit

first Pikro_00020