Pikro_00020 : CDS information

Location

Organism	Streptomyces venezuelae
Strain	ATCC 15439
Entry name	Pikromycin
Contig	AF079138
Start / Stop / Direction	2,293 / 1,283 / - [in whole cluster] 2,293 / 1,283 / - [in contig]
Location	complement(1283..2293) [in whole cluster] complement(1283..2293) [in contig]
Type	CDS
Length	1,011 bp (336 aa)

Annotation

Category	4.4 resistance
Product	23S rRNA adenine N-6-methyltransferase
Product (GenBank)	rRNA methyltransferase PikR1
Gene
Gene (GenBank)	pikR1
EC number	2.1.1.-
Keyword
Note
Note (GenBank)	mls type resistance protein
Reference	ACC Q9ZGI6 PmId [9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998) [26438831] Resistance to ketolide antibiotics by coordinated expression of rRNA methyltransferases in a bacterial producer of natural ketolides. (Proc Natl Acad Sci U S A. , 2015) comment [PMID: 9770448](1998) 12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。 PikR1: rRNA methyltransferase (mls resistance) pikR1 and pikR2はmacrolide-lincosamide-streptogramin B type resistant genesであり、たぶん細胞の自己防衛を供給する。 --- [PMID: 26438831](2015) PikR1 and PikR2 enzymesはそれぞれ、23S rRNA nucleotide A2058におけるN6アミノ基をmono- and dimethylateすることを確認。
Related Reference	ACC Q6RX35 PmId [15590712] Molecular basis of intrinsic macrolide resistance in clinical isolates of Mycobacterium fortuitum. (J Antimicrob Chemother. , 2005) comment BLAST id47% Mycobacterium fortuitum Erm(39) M. fortuitum rRNA methylase gene erm(39)を同定。 erm(39)はmacrolide-lincosamide agentsへの抵抗性を与えたが(MIC >128 mg/L)、streptogramin Bには与えなかった。
Related Reference	ACC P07287 PmId [7961464] Domain V of 23S rRNA contains all the structural elements necessary for recognition by the ErmE methyltransferase. (J Bacteriol. , 1994) [7793855] Erythromycin resistance by ribosome modification. (Antimicrob Agents Chemother. , 1995) comment BLAST id33% Saccharopolyspora erythraea (strain NRRL 23338)_ermE rRNA adenine N-6-methyltransferase(EC 2.1.1.48) 抗生物質抵抗性、23S rRNA modificationによるmethyltransferase発現測定。 virtoでのmethylation assay.

Sequence

>23S rRNA adenine N-6-methyltransferase [rRNA methyltransferase PikR1]
MAMRDSIPRRADRDTLRRELGQNFLQDDRAVRNLVTHVEGDGRNVLEIGPGKGAITEELV
RSFDTVTVVEMDPHWAAHVRRKFEGERVTVFQGDFLDFRIPRDIDTVVGNVPFGITTQIL
RSLLESTNWQSAALIVQWEVARKRAGRSGGSLLTTSWAPWYEFAVHDRVRASSFRPMPRV
DGGVLTIRRRPQPLLPESASRAFQNFAEAVFTGPGRGLAEILRRHIPKRTYRSLADRHGI
PDGGLPKDLTLTQWIALFQASQPSYAPGAPGTRMPGQGGGAGGRDYDSETSRAAVPGSRR
YGPTRGGEPCAPRAQVRQTKGRQGARGSSYGRRTGR

>23S rRNA adenine N-6-methyltransferase [rRNA methyltransferase PikR1]
ATGGCAATGCGCGACTCCATACCGAGGCGAGCGGACCGCGACACCCTTCGCCGCGAATTA
GGCCAGAACTTCCTTCAGGACGACAGAGCCGTGCGCAATCTCGTCACGCATGTCGAGGGG
GACGGTAGGAACGTTCTCGAAATCGGCCCCGGAAAGGGCGCGATAACCGAGGAGTTGGTG
CGCTCCTTCGACACCGTGACGGTCGTGGAGATGGACCCGCACTGGGCCGCGCATGTGCGG
CGGAAATTCGAAGGGGAGAGGGTCACCGTATTCCAGGGTGATTTCCTCGACTTCCGCATT
CCGCGCGATATCGACACCGTCGTCGGAAACGTTCCCTTCGGCATCACGACCCAGATTCTC
CGGAGTCTCCTGGAATCGACGAACTGGCAGTCGGCGGCCCTGATAGTGCAGTGGGAGGTC
GCCCGCAAACGCGCCGGTCGCAGCGGCGGATCGCTCCTCACGACCTCCTGGGCCCCCTGG
TACGAGTTCGCGGTCCACGACCGCGTCCGCGCCTCGTCGTTCCGTCCGATGCCCCGCGTC
GACGGCGGCGTCCTGACGATCAGGCGACGCCCCCAGCCCCTGCTGCCCGAGAGCGCGAGC
CGCGCCTTCCAGAACTTCGCCGAAGCCGTCTTCACCGGCCCCGGACGGGGCCTCGCGGAG
ATCCTCCGGCGCCACATCCCCAAGCGGACCTACCGTTCCCTCGCCGACCGCCACGGAATT
CCGGACGGCGGACTGCCGAAGGACCTCACGCTCACCCAATGGATCGCCCTTTTCCAGGCC
TCCCAGCCGAGTTACGCGCCGGGGGCGCCCGGCACGCGCATGCCGGGCCAGGGCGGTGGC
GCCGGCGGCAGGGACTATGACTCGGAGACGAGCAGGGCCGCCGTGCCCGGGAGCCGCAGA
TACGGCCCCACGCGCGGCGGCGAACCCTGCGCACCCCGCGCACAGGTCCGGCAGACCAAG
GGCCGCCAGGGCGCGCGAGGCTCGTCGTACGGACGCCGCACGGGCCGTTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001737 Ribosomal RNA adenine methylase transferase (Family) PF00398 RrnaAD IPR020596 Ribosomal RNA adenine methylase transferase, conserved site (Conserved_site) PS01131 RRNA_A_DIMETH IPR020598 Ribosomal RNA adenine methylase transferase, N-terminal (Domain) SM00650 rADc
SignalP	No significant hit
TMHMM	No significant hit

Pikro_00010 Pikro_00030