Pikro_00030 Pikro_00030   Pikro_00050 Pikro_00050

Pikro_00040 : CDS information

close this sectionLocation

Organism
StrainATCC 15439
Entry namePikromycin
Contig
Start / Stop / Direction16,858 / 28,077 / + [in whole cluster]
16,858 / 28,077 / + [in contig]
Location16858..28077 [in whole cluster]
16858..28077 [in contig]
TypeCDS
Length11,220 bp (3,739 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase
Product (GenBank)type I polyketide synthase PikAII
Gene
Gene (GenBank)pikAII
EC number
Keyword
Note
Note (GenBank)
  • multifunctional polyketide synthase; contains Pik module 3 and module 4
Reference
ACC
PmId
[9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998)
[10421766] Elucidating the mechanism of chain termination switching in the picromycin/methymycin polyketide synthase. (Chem Biol. , 1999)
[10662693] Formation of functional heterologous complexes using subunits from the picromycin, erythromycin and oleandomycin polyketide synthases. (Chem Biol. , 2000)
[10713461] Genetic architecture of the polyketide synthases for methymycin and pikromycin series macrolides. (Gene. , 2000)
[25004372] Elucidation of the cryptic epimerase activity of redox-inactive ketoreductase domains from modular polyketide synthases by tandem equilibrium isotope exchange. (J Am Chem Soc. , 2014)
[26863427] Epimerase and Reductase Activities of Polyketide Synthase Ketoreductase Domains Utilize the Same Conserved Tyrosine and Serine Residues. (Biochemistry. , 2016)
comment
[PMID: 9770448](1998)
12員環骨格を持つmethymycin, neomethymycinと、14員環骨格を持つnarbomycin, pikromycinの生合成で共有されるclusterの同定。

PikAII (3,739bp): PKS
Module 3; KS, AT(P), KR0, ACP
Module 4; KS, AT(P), DH, ER, KR, ACP

KR0 = inactive KR

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[PMID: 10421766](1999)
pikAI-AIVをS. lividansで異種性発現し、12員環前駆体10-deoxymethynolideと14員環前駆体narbonolideの産生を確認。Pik TEII thioesterase(pikAV)も同時に発現させると産生レベルは増加するが、2つの化合物の比率は変わらない。

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[PMID: 10662693](2000)
pikAI, pikAII をクローニングし、erythromycinとoleandomycinのPKS genesと組み合わせて産物を確認している。

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[PMID: 10713461](2000)
・12員環＋14員環マクロライドを産生するS. venezuelae ATCC 15439
・主に12員環マクロライドを産生するS. venezuelae ATCC 15068
・14員環マクロライドのみを産生するS. narbonensis ATCC 19790
の3株のPKSの配列解析から、その産生調節の違いを検討している。

S. venezuelae ATCC 15439のpik clusterの5つの遺伝子座のうちPKSのpikAをシークエンスし、遺伝子構成を示している。

---
[PMID: 25004372](2014)
KR domainsに相同性が高いが酸化還元のできない機能不明なKR(0) domainについて調査。
KR(0) domainは内因性epimerase活性を持つことがEryKR3(0)、PicKR3(0)を用いて確認された。

close this sectionPKS/NRPS Module

3 methylmalonyl-CoA
4 methylmalonyl-CoA
KS36..412
AT572..881
kr1150..1345
ACP1443..1517
KS1539..1916
AT2069..2379
DH2428..2593
ER2959..3267
KR3277..3459
ACP3572..3642

close this sectionSequence

selected fasta
>polyketide synthase [type I polyketide synthase PikAII]
MSTVNEEKYLDYLRRATADLHEARGRLRELEAKAGEPVAIVGMACRLPGGVASPEDLWRL
VAGGEDAISEFPQDRGWDVEGLYDPNPEATGKSYAREAGFLYEAGEFDADFFGISPREAL
AMDPQQRLLLEASWEAFEHAGIPAATARGTSVGVFTGVMYHDYATRLTDVPEGIEGYLGT
GNSGSVASGRVAYTLGLEGPAVTVDTACSSSLVALHLAVQALRKGEVDMALAGGVTVMST
PSTFVEFSRQRGLAPDGRSKSFSSTADGTSWSEGVGVLLVERLSDARRKGHRILAVVRGT
AVNQDGASSGLTAPNGPSQQRVIRRALADARLTTSDVDVVEAHGTGTRLGDPIEAQAVIA
TYGQGRDGEQPLRLGSLKSNIGHTQAAAGVSGVIKMVQAMRHGVLPKTLHVEKPTDQVDW
SAGAVELLTEAMDWPDKGDGGLRRAAVSSFGVSGTNAHVVLEEAPAAEETPASEATPAVE
PSVGAGLVPWLVSAKTPAALDAQIGRLAAFASQGRTDAADPGAVARVLAGGRAEFEHRAV
VLGTGQDDFAQALTAPEGLIRGTPSDVGRVAFVFPGQGTQWAGMGAELLDVSKEFAAAMA
ECESALSRYVDWSLEAVVRQAPGAPTLERVDVVQPVTFAVMVSLAKVWQHHGVTPQAVVG
HSQGEIAAAYVAGALTLDDAARVVTLRSKSIAAHLAGKGGMISLALSEEATRQRIENLHG
LSIAAVNGPTATVVSGDPTQIQELAQACEADGVRARIIPVDYASHSAHVETIESELAEVL
AGLSPRTPEVPFFSTLEGAWITEPVLDGTYWYRNLRHRVGFAPAVETLATDEGFTHFIEV
SAHPVLTMTLPETVTGLGTLRREQGGQERLVTSLAEAWTNGLTIDWAPVLPTATGHHPEL
PTYAFQRRHYWLHDSPAVQGSVQDSWRYRIDWKRLAVADASERAGLSGRWLVVVPEDRSA
EAAPVLAALSGAGADPVQLDVSPLGDRQRLAATLGEALAAAGGAVDGVLSLLAWDESAHP
GHPAPFTRGTGATLTLVQALEDAGVAAPLWCVTHGAVSVGRADHVTSPAQAMVWGMGRVA
ALEHPERWGGLIDLPSDADRAALDRMTTVLAGGTGEDQVAVRASGLLARRLVRASLPAHG
TASPWWQADGTVLVTGAEEPAAAEAARRLARDGAGHLLLHTTPSGSEGAEGTSGAAEDSG
LAGLVAELADLGATATVVTCDLTDAEAAARLLAGVSDAHPLSAVLHLPPTVDSEPLAATD
ADALARVVTAKATAALHLDRLLREAAAAGGRPPVLVLFSSVAAIWGGAGQGAYAAGTAFL
DALAGQHRADGPTVTSVAWSPWEGSRVTEGATGERLRRLGLRPLAPATALTALDTALGHG
DTAVTIADVDWSSFAPGFTTARPGTLLADLPEARRALDEQQSTTAADDTVLSRELGALTG
AEQQRRMQELVREHLAVVLNHPSPEAVDTGRAFRDLGFDSLTAVELRNRLKNATGLALPA
TLVFDYPTPRTLAEFLLAEILGEQAGAGEQLPVDGGVDDEPVAIVGMACRLPGGVASPED
LWRLVAGGEDAISGFPQDRGWDVEGLYDPDPDASGRTYCRAGGFLDEAGEFDADFFGISP
REALAMDPQQRLLLETSWEAVEDAGIDPTSLQGQQVGVFAGTNGPHYEPLLRNTAEDLEG
YVGTGNAASIMSGRVSYTLGLEGPAVTVDTACSSSLVALHLAVQALRKGECGLALAGGVT
VMSTPTTFVEFSRQRGLAEDGRSKAFAASADGFGPAEGVGMLLVERLSDARRNGHRVLAV
VRGSAVNQDGASNGLTAPNGPSQQRVIRRALADARLTTADVDVVEAHGTGTRLGDPIEAQ
ALIATYGQGRDTEQPLRLGSLKSNIGHTQAAAGVSGIIKMVQAMRHGVLPKTLHVDRPSD
QIDWSAGTVELLTEAMDWPRKQEGGLRRAAVSSFGISGTNAHIVLEEAPVDEDAPADEPS
VGGVVPWLVSAKTPAALDAQIGRLAAFASQGRTDAADPGAVARVLAGGRAQFEHRAVALG
TGQDDLAAALAAPEGLVRGVASGVGRVAFVFPGQGTQWAGMGAELLDVSKEFAAAMAECE
AALAPYVDWSLEAVVRQAPGAPTLERVDVVQPVTFAVMVSLAKVWQHHGVTPQAVVGHSQ
GEIAAAYVAGALSLDDAARVVTLRSKSIGAHLAGQGGMLSLALSEAAVVERLAGFDGLSV
AAVNGPTATVVSGDPTQIQELAQACEADGVRARIIPVDYASHSAHVETIESELADVLAGL
SPQTPQVPFFSTLEGAWITEPALDGGYWYRNLRHRVGFAPAVETLATDEGFTHFVEVSAH
PVLTMALPETVTGLGTLRRDNGGQHRLTTSLAEAWANGLTVDWASLLPTTTTHPDLPTYA
FQTERYWPQPDLSAAGDITSAGLGAAEHPLLGAAVALADSDGCLLTGSLSLRTHPWLADH
AVAGTVLLPGTAFVELAFRAGDQVGCDLVEELTLDAPLVLPRRGAVRVQLSVGASDESGR
RTFGLYAHPEDAPGEAEWTRHATGVLAARADRTAPVADPEAWPPPGAEPVDVDGLYERFA
ANGYGYGPLFQGVRGVWRRGDEVFADVALPAEVAGAEGARFGLHPALLDAAVQAAGAGRG
VRRGHAAAVRLERDLLYAVGATALRVRLAPAGPDTVSVSAADSSGQPVFAADSLTVLPVD
PAQLAAFSDPTLDALHLLEWTAWDGAAQALPGAVVLGGDADGLAAALRAGGTEVLSFPDL
TDLVEAVDRGETPAPATVLVACPAAGPDGPEHVREALHGSLALMQAWLADERFTDGRLVL
VTRDAVAARSGDGLRSTGQAAVWGLGRSAQTESPGRFVLLDLAGEARTAGDATAGDGLTT
GDATVGGTSGDAALGSALATALGSGEPQLALRDGALLVPRLARAAAPAAADGLAAADGLA
ALPLPAAPALWRLEPGTDGSLESLTAAPGDAETLAPEPLGPGQVRIAIRATGLNFRDVLI
ALGMYPDPALMGTEGAGVVTATGPGVTHLAPGDRVMGLLSGAYAPVVVADARTVARMPEG
WTFAQGASVPVVFLTAVYALRDLADVKPGERLLVHSAAGGVGMAAVQLARHWGVEVHGTA
SHGKWDALRALGLDDAHIASSRTLDFESAFRAASGGAGMDVVLNSLAREFVDASLRLLGP
GGRFVEMGKTDVRDAERVAADHPGVGYRAFDLGEAGPERIGEMLAEVIALFEDGVLRHLP
VTTWDVRRARDAFRHVSQARHTGKVVLTMPSGLDPEGTVLLTGGTGALGGIVARHVVGEW
GVRRLLLVSRRGTDAPGAGELVHELEALGADVSVAACDVADREALTAVLDSIPAEHPLTA
VVHTAGVLSDGTLPSMTAEDVEHVLRPKVDAAFLLDELTSTPGYDLAAFVMFSSAAAVFG
GAGQGAYAAANATLDALAWRRRTAGLPALSLGWGLWAETSGMTGGLSDTDRSRLARSGAT
PMDSELTLSLLDAAMRRDDPALVPIALDVAALRAQQRDGMLAPLLSGLTRGSRVGGAPVN
QRRAAAGGAGEADTDLGGRLAAMTPDDRVAHLRDLVRTHVATVLGHGTPSRVDLERAFRD
TGFDSLTAVELRNRLNAATGLRLPATLVFDHPTPGELAGHLLDELATAAGGSWAEGTGSG
DTASATDRQTTAALAELDRLEGVLASLAPAAGGRPELAARLRALAAALGDDGDDATDLDE
ASDDDLFSFIDKELGDSDF
selected fasta
>polyketide synthase [type I polyketide synthase PikAII]
GTGTCCACGGTGAACGAAGAGAAGTACCTCGACTACCTGCGTCGTGCCACGGCGGACCTC
CACGAGGCCCGTGGCCGCCTCCGCGAGCTGGAGGCGAAGGCGGGCGAGCCGGTGGCGATC
GTCGGCATGGCCTGCCGCCTGCCCGGCGGCGTCGCCTCGCCCGAGGACCTGTGGCGGCTG
GTGGCCGGCGGCGAGGACGCGATCTCGGAGTTCCCCCAGGACCGCGGCTGGGACGTGGAG
GGCCTGTACGACCCGAACCCGGAGGCCACGGGCAAGAGTTACGCCCGCGAGGCCGGATTC
CTGTACGAGGCGGGCGAGTTCGACGCCGACTTCTTCGGGATCTCGCCGCGCGAGGCCCTC
GCCATGGACCCGCAGCAGCGTCTCCTCCTGGAGGCCTCCTGGGAGGCGTTCGAGCACGCC
GGGATCCCGGCGGCCACCGCGCGCGGCACCTCGGTCGGCGTCTTCACCGGCGTGATGTAC
CACGACTACGCCACCCGTCTCACCGATGTCCCGGAGGGCATCGAGGGCTACCTGGGCACC
GGCAACTCCGGCAGTGTCGCCTCGGGCCGCGTCGCGTACACGCTTGGCCTGGAGGGGCCG
GCCGTCACGGTCGACACCGCCTGCTCGTCCTCGCTGGTCGCCCTGCACCTCGCCGTGCAG
GCCCTGCGCAAGGGCGAGGTCGACATGGCGCTCGCCGGCGGCGTGACGGTCATGTCGACG
CCCAGCACCTTCGTCGAGTTCAGCCGTCAGCGCGGGCTGGCGCCGGACGGCCGGTCGAAG
TCCTTCTCGTCGACGGCCGACGGCACCAGCTGGTCCGAGGGCGTCGGCGTCCTCCTCGTC
GAGCGCCTGTCCGACGCGCGTCGCAAGGGCCATCGGATCCTCGCCGTGGTCCGGGGCACC
GCCGTCAACCAGGACGGCGCCAGCAGCGGCCTCACGGCTCCGAACGGGCCGTCGCAGCAG
CGCGTCATCCGACGTGCCCTGGCGGACGCCCGGCTCACGACCTCCGACGTGGACGTCGTC
GAGGCCCACGGCACGGGTACGCGACTCGGCGACCCGATCGAGGCGCAGGCCGTCATCGCC
ACGTACGGGCAGGGCCGTGACGGCGAACAGCCGCTGCGCCTCGGGTCGTTGAAGTCCAAC
ATCGGACACACCCAGGCCGCCGCCGGTGTCTCCGGCGTGATCAAGATGGTCCAGGCGATG
CGCCACGGCGTCCTGCCGAAGACGCTCCACGTGGAGAAGCCGACGGACCAGGTGGACTGG
TCCGCGGGCGCGGTCGAGCTGCTCACCGAGGCCATGGACTGGCCGGACAAGGGCGACGGC
GGACTGCGCAGGGCCGCGGTCTCCTCCTTCGGCGTCAGCGGGACGAACGCGCACGTCGTG
CTCGAAGAGGCCCCGGCGGCCGAGGAGACCCCTGCCTCCGAGGCGACCCCGGCCGTCGAG
CCGTCGGTCGGCGCCGGCCTGGTGCCGTGGCTGGTGTCGGCGAAGACTCCGGCCGCGCTG
GACGCCCAGATCGGACGCCTCGCCGCGTTCGCCTCGCAGGGCCGTACGGACGCCGCCGAT
CCGGGCGCGGTCGCTCGCGTACTGGCCGGCGGGCGCGCCGAGTTCGAGCACCGGGCCGTC
GTGCTCGGCACCGGACAGGACGATTTCGCGCAGGCGCTGACCGCTCCGGAAGGACTGATA
CGCGGCACGCCCTCGGACGTGGGCCGGGTGGCGTTCGTGTTCCCCGGTCAGGGCACGCAG
TGGGCCGGGATGGGCGCCGAACTCCTCGACGTGTCGAAGGAGTTCGCGGCGGCCATGGCC
GAGTGCGAGAGCGCGCTCTCCCGCTATGTCGACTGGTCGCTGGAGGCCGTCGTCCGGCAG
GCGCCGGGCGCGCCCACGCTGGAGCGGGTCGACGTCGTCCAGCCCGTGACCTTCGCTGTC
ATGGTTTCGCTGGCGAAGGTCTGGCAGCACCACGGCGTGACGCCGCAGGCCGTCGTCGGC
CACTCGCAGGGCGAGATCGCCGCCGCGTACGTCGCCGGTGCCCTCACCCTCGACGACGCC
GCCCGCGTCGTCACCCTGCGCAGCAAGTCCATCGCCGCCCACCTCGCCGGCAAGGGCGGC
ATGATCTCCCTCGCCCTCAGCGAGGAAGCCACCCGGCAGCGCATCGAGAACCTCCACGGA
CTGTCGATCGCCGCCGTCAACGGCCCCACCGCCACCGTGGTTTCGGGCGACCCCACCCAG
ATCCAAGAGCTCGCTCAGGCGTGTGAGGCCGACGGGGTCCGCGCACGGATCATCCCCGTC
GACTACGCCTCCCACAGCGCCCACGTCGAGACCATCGAGAGCGAACTCGCCGAGGTCCTC
GCCGGGCTCAGCCCGCGGACACCTGAGGTGCCGTTCTTCTCGACACTCGAAGGCGCCTGG
ATCACCGAGCCGGTGCTCGACGGCACCTACTGGTACCGCAACCTCCGCCACCGCGTCGGC
TTCGCCCCCGCCGTCGAGACCCTCGCCACCGACGAAGGCTTCACCCACTTCATCGAGGTC
AGCGCCCACCCCGTCCTCACCATGACCCTCCCCGAGACCGTCACCGGCCTCGGCACCCTC
CGCCGCGAACAGGGAGGCCAGGAGCGTCTGGTCACCTCACTCGCCGAAGCCTGGACCAAC
GGCCTCACCATCGACTGGGCGCCCGTCCTCCCCACCGCAACCGGCCACCACCCCGAGCTC
CCCACCTACGCCTTCCAGCGCCGTCACTACTGGCTCCACGACTCCCCCGCCGTCCAGGGC
TCCGTGCAGGACTCCTGGCGCTACCGCATCGACTGGAAGCGCCTCGCGGTCGCCGACGCG
TCCGAGCGCGCCGGGCTGTCCGGGCGCTGGCTCGTCGTCGTCCCCGAGGACCGTTCCGCC
GAGGCCGCCCCGGTGCTCGCCGCGCTGTCCGGCGCCGGCGCCGACCCCGTACAGCTGGAC
GTGTCCCCGCTGGGCGACCGGCAGCGGCTCGCCGCGACGCTGGGCGAGGCCCTGGCGGCG
GCCGGTGGAGCCGTCGACGGCGTCCTCTCGCTGCTCGCGTGGGACGAGAGCGCGCACCCC
GGCCACCCCGCCCCCTTCACCCGGGGCACCGGCGCCACCCTCACCCTGGTGCAGGCGCTG
GAGGACGCCGGCGTCGCCGCCCCGCTGTGGTGCGTGACCCACGGCGCGGTGTCCGTCGGC
CGGGCCGACCACGTCACCTCCCCCGCCCAGGCCATGGTGTGGGGCATGGGCCGGGTCGCC
GCCCTGGAGCACCCCGAGCGGTGGGGCGGCCTGATCGACCTGCCCTCGGACGCCGACCGG
GCGGCCCTGGACCGCATGACCACGGTCCTCGCCGGCGGTACGGGTGAGGACCAGGTCGCG
GTACGCGCCTCCGGGCTGCTCGCCCGCCGCCTCGTCCGCGCCTCCCTCCCGGCGCACGGC
ACGGCTTCGCCGTGGTGGCAGGCCGACGGCACGGTGCTCGTCACCGGTGCCGAGGAGCCT
GCGGCCGCCGAGGCCGCACGCCGGCTGGCCCGCGACGGCGCCGGACACCTCCTCCTCCAC
ACCACCCCCTCCGGCAGCGAAGGCGCCGAAGGCACCTCCGGTGCCGCCGAGGACTCCGGC
CTCGCCGGGCTCGTCGCCGAACTCGCGGACCTGGGCGCGACGGCCACCGTCGTGACCTGC
GACCTCACGGACGCGGAGGCGGCCGCCCGGCTGCTCGCCGGCGTCTCCGACGCGCACCCG
CTCAGCGCCGTCCTCCACCTGCCGCCCACCGTCGACTCCGAGCCGCTCGCCGCGACCGAC
GCGGACGCGCTCGCCCGTGTCGTGACCGCGAAGGCCACCGCCGCGCTCCACCTGGACCGC
CTCCTGCGGGAGGCCGCGGCTGCCGGAGGCCGTCCGCCCGTCCTGGTCCTCTTCTCCTCG
GTCGCCGCGATCTGGGGCGGCGCCGGTCAGGGCGCGTACGCCGCCGGTACGGCCTTCCTC
GACGCCCTCGCCGGTCAGCACCGGGCCGACGGCCCCACCGTGACCTCGGTGGCCTGGAGC
CCCTGGGAGGGCAGCCGCGTCACCGAGGGTGCGACCGGGGAGCGGCTGCGCCGCCTCGGC
CTGCGCCCCCTCGCCCCCGCGACGGCGCTCACCGCCCTGGACACCGCGCTCGGCCACGGC
GACACCGCCGTCACGATCGCCGACGTCGACTGGTCGAGCTTCGCCCCCGGCTTCACCACG
GCCCGGCCGGGCACCCTCCTCGCCGATCTGCCCGAGGCGCGCCGCGCGCTCGACGAGCAG
CAGTCGACGACGGCCGCCGACGACACCGTCCTGAGCCGCGAGCTCGGTGCGCTCACCGGC
GCCGAACAGCAGCGCCGTATGCAGGAGTTGGTCCGCGAGCACCTCGCCGTGGTCCTCAAC
CACCCCTCCCCCGAGGCCGTCGACACGGGGCGGGCCTTCCGTGACCTCGGATTCGACTCG
CTGACGGCGGTCGAGCTCCGCAACCGCCTCAAGAACGCCACCGGCCTGGCCCTCCCGGCC
ACTCTGGTCTTCGACTACCCGACCCCCCGGACGCTGGCGGAGTTCCTCCTCGCGGAGATC
CTGGGCGAGCAGGCCGGTGCCGGCGAGCAGCTTCCGGTGGACGGCGGGGTCGACGACGAG
CCCGTCGCGATCGTCGGCATGGCGTGCCGCCTGCCGGGCGGTGTCGCCTCGCCGGAGGAC
CTGTGGCGGCTGGTGGCCGGCGGCGAGGACGCGATCTCCGGCTTCCCGCAGGACCGCGGC
TGGGACGTGGAGGGGCTGTACGACCCGGACCCGGACGCGTCCGGGCGGACGTACTGCCGT
GCCGGTGGCTTCCTCGACGAGGCGGGCGAGTTCGACGCCGACTTCTTCGGGATCTCGCCG
CGCGAGGCCCTCGCCATGGACCCGCAGCAGCGGCTCCTCCTGGAGACCTCCTGGGAGGCC
GTCGAGGACGCCGGGATCGACCCGACCTCCCTTCAGGGGCAGCAGGTCGGCGTGTTCGCG
GGCACCAACGGCCCCCACTACGAGCCGCTGCTCCGCAACACCGCCGAGGATCTTGAGGGT
TACGTCGGGACGGGCAACGCCGCCAGCATCATGTCGGGCCGTGTCTCGTACACCCTCGGC
CTGGAGGGCCCGGCCGTCACGGTCGACACCGCCTGCTCCTCCTCGCTGGTCGCCCTGCAC
CTCGCCGTGCAGGCCCTGCGCAAGGGCGAATGCGGACTGGCGCTCGCGGGCGGTGTGACG
GTCATGTCGACGCCCACGACGTTCGTGGAGTTCAGCCGGCAGCGCGGGCTCGCGGAGGAC
GGCCGGTCGAAGGCGTTCGCCGCGTCGGCGGACGGCTTCGGCCCGGCGGAGGGCGTCGGC
ATGCTCCTCGTCGAGCGCCTGTCGGACGCCCGCCGCAACGGACACCGTGTGCTGGCGGTC
GTGCGCGGCAGCGCGGTCAACCAGGACGGCGCGAGCAACGGCCTGACCGCCCCGAACGGG
CCCTCGCAGCAGCGCGTCATCCGGCGCGCGCTCGCGGACGCCCGACTGACGACCGCCGAC
GTGGACGTCGTCGAGGCCCACGGCACGGGCACGCGACTCGGCGACCCGATCGAGGCACAG
GCCCTCATCGCCACCTACGGCCAGGGGCGCGACACCGAACAGCCGCTGCGCCTGGGGTCG
TTGAAGTCCAACATCGGACACACCCAGGCCGCCGCCGGTGTCTCCGGCATCATCAAGATG
GTCCAGGCGATGCGCCACGGCGTCCTGCCGAAGACGCTCCACGTGGACCGGCCGTCGGAC
CAGATCGACTGGTCGGCGGGCACGGTCGAGCTGCTCACCGAGGCCATGGACTGGCCGAGG
AAGCAGGAGGGCGGGCTGCGCCGCGCGGCCGTCTCCTCCTTCGGCATCAGCGGCACGAAC
GCGCACATCGTGCTCGAAGAAGCCCCGGTCGACGAGGACGCCCCGGCGGACGAGCCGTCG
GTCGGCGGTGTGGTGCCGTGGCTCGTGTCCGCGAAGACTCCGGCCGCGCTGGACGCCCAG
ATCGGACGCCTCGCCGCGTTCGCCTCGCAGGGCCGTACGGACGCCGCCGATCCGGGCGCG
GTCGCTCGCGTACTGGCCGGCGGGCGTGCGCAGTTCGAGCACCGGGCCGTCGCGCTCGGC
ACCGGACAGGACGACCTGGCGGCCGCACTGGCCGCGCCTGAGGGTCTGGTCCGGGGTGTG
GCCTCCGGTGTGGGTCGAGTGGCGTTCGTGTTCCCGGGACAGGGCACGCAGTGGGCCGGG
ATGGGTGCCGAACTCCTCGACGTGTCGAAGGAGTTCGCGGCGGCCATGGCCGAGTGCGAG
GCCGCGCTCGCTCCGTACGTGGACTGGTCGCTGGAGGCCGTCGTCCGACAGGCCCCCGGC
GCGCCCACGCTGGAGCGGGTCGATGTCGTCCAGCCCGTGACGTTCGCCGTCATGGTCTCG
CTGGCGAAGGTCTGGCAGCACCACGGGGTGACCCCGCAAGCCGTCGTCGGCCACTCGCAG
GGCGAGATCGCCGCCGCGTACGTCGCCGGTGCCCTGAGCCTGGACGACGCCGCTCGTGTC
GTGACCCTGCGCAGCAAGTCCATCGGCGCCCACCTCGCGGGCCAGGGCGGCATGCTGTCC
CTCGCGCTGAGCGAGGCGGCCGTTGTGGAGCGACTGGCCGGGTTCGACGGGCTGTCCGTC
GCCGCCGTCAACGGGCCTACCGCCACCGTGGTTTCGGGCGACCCGACCCAGATCCAAGAG
CTCGCTCAGGCGTGTGAGGCCGACGGGGTCCGCGCACGGATCATCCCCGTCGACTACGCC
TCCCACAGCGCCCACGTCGAGACCATCGAGAGCGAACTCGCCGACGTCCTGGCGGGGTTG
TCCCCCCAGACACCCCAGGTCCCCTTCTTCTCCACCCTCGAAGGCGCCTGGATCACCGAA
CCCGCCCTCGACGGCGGCTACTGGTACCGCAACCTCCGCCATCGTGTGGGCTTCGCCCCG
GCCGTCGAAACCCTGGCCACCGACGAAGGCTTCACCCACTTCGTCGAGGTCAGCGCCCAC
CCCGTCCTCACCATGGCGCTGCCCGAGACCGTCACCGGACTCGGCACCCTCCGCCGTGAC
AACGGCGGACAGCACCGCCTCACCACCTCCCTCGCCGAGGCCTGGGCCAACGGCCTCACC
GTCGACTGGGCCTCTCTCCTCCCCACCACGACCACCCACCCCGATCTGCCCACCTACGCC
TTCCAGACCGAGCGCTACTGGCCGCAGCCCGACCTCTCCGCCGCCGGTGACATCACCTCC
GCCGGTCTCGGGGCGGCCGAGCACCCGCTGCTCGGCGCGGCCGTGGCGCTCGCGGACTCC
GACGGCTGCCTGCTCACGGGGAGCCTCTCCCTCCGTACGCACCCCTGGCTGGCGGACCAC
GCGGTGGCCGGCACCGTGCTGCTGCCGGGAACGGCGTTCGTGGAGCTGGCGTTCCGAGCC
GGGGACCAGGTCGGTTGCGATCTGGTCGAGGAGCTCACCCTCGACGCGCCGCTCGTGCTG
CCCCGTCGTGGCGCGGTCCGTGTGCAGCTGTCCGTCGGCGCGAGCGACGAGTCCGGGCGT
CGTACCTTCGGGCTCTACGCGCACCCGGAGGACGCGCCGGGCGAGGCGGAGTGGACGCGG
CACGCCACCGGTGTGCTGGCCGCCCGTGCGGACCGCACCGCCCCCGTCGCCGACCCGGAG
GCCTGGCCGCCGCCGGGCGCCGAGCCGGTGGACGTGGACGGTCTGTACGAGCGCTTCGCG
GCGAACGGCTACGGCTACGGCCCCCTCTTCCAGGGCGTCCGTGGTGTCTGGCGGCGTGGC
GACGAGGTGTTCGCCGACGTGGCCCTGCCGGCCGAGGTCGCCGGTGCCGAGGGCGCGCGG
TTCGGCCTTCACCCGGCGCTGCTCGACGCCGCCGTGCAGGCGGCCGGTGCGGGCCGGGGC
GTTCGGCGCGGGCACGCGGCTGCCGTTCGCCTGGAGCGGGATCTCCTGTACGCGGTCGGC
GCCACCGCCCTCCGCGTGCGGCTGGCCCCCGCCGGCCCGGACACGGTGTCCGTGAGCGCC
GCCGACTCCTCCGGGCAGCCGGTGTTCGCCGCGGACTCCCTCACGGTGCTGCCCGTCGAC
CCCGCGCAGCTGGCGGCCTTCAGCGACCCGACTCTGGACGCGCTGCACCTGCTGGAGTGG
ACCGCCTGGGACGGTGCCGCGCAGGCCCTGCCCGGCGCGGTCGTGCTGGGCGGCGACGCC
GACGGTCTCGCCGCGGCGCTGCGCGCCGGTGGCACCGAGGTCCTGTCCTTCCCGGACCTT
ACGGACCTGGTGGAGGCCGTCGACCGGGGCGAGACCCCGGCCCCGGCGACCGTCCTGGTG
GCCTGCCCCGCCGCCGGCCCCGATGGGCCGGAGCATGTCCGCGAGGCCCTGCACGGGTCG
CTCGCGCTGATGCAGGCCTGGCTGGCCGACGAGCGGTTCACCGATGGGCGCCTGGTGCTC
GTGACCCGCGACGCGGTCGCCGCCCGTTCCGGCGACGGCCTGCGGTCCACGGGACAGGCC
GCCGTCTGGGGCCTCGGCCGGTCCGCGCAGACGGAGAGCCCGGGCCGGTTCGTCCTGCTC
GACCTCGCCGGGGAAGCCCGGACGGCCGGGGACGCCACCGCCGGGGACGGCCTGACGACC
GGGGACGCCACCGTCGGCGGCACCTCTGGAGACGCCGCCCTCGGCAGCGCCCTCGCGACC
GCCCTCGGCTCGGGCGAGCCGCAGCTCGCCCTCCGGGACGGGGCGCTCCTCGTACCCCGC
CTGGCGCGGGCCGCCGCGCCCGCCGCGGCCGACGGCCTCGCCGCGGCCGACGGCCTCGCC
GCTCTGCCGCTGCCCGCCGCTCCGGCCCTCTGGCGTCTGGAGCCCGGTACGGACGGCAGC
CTGGAGAGCCTCACGGCGGCGCCCGGCGACGCCGAGACCCTCGCCCCGGAGCCGCTCGGC
CCGGGACAGGTCCGCATCGCGATCCGGGCCACCGGTCTCAACTTCCGCGACGTCCTGATC
GCCCTCGGCATGTACCCCGATCCGGCGCTGATGGGCACCGAGGGAGCCGGCGTGGTCACC
GCGACCGGCCCCGGCGTCACGCACCTCGCCCCCGGCGACCGGGTCATGGGCCTGCTCTCC
GGCGCGTACGCCCCGGTCGTCGTGGCGGACGCGCGGACCGTCGCGCGGATGCCCGAGGGG
TGGACGTTCGCCCAGGGCGCCTCCGTGCCGGTGGTGTTCCTGACGGCCGTCTACGCCCTG
CGCGACCTGGCGGACGTCAAGCCCGGCGAGCGCCTCCTGGTCCACTCCGCCGCCGGTGGC
GTGGGCATGGCCGCCGTGCAGCTCGCCCGGCACTGGGGCGTGGAGGTCCACGGCACGGCG
AGTCACGGGAAGTGGGACGCCCTGCGCGCGCTCGGCCTGGACGACGCGCACATCGCCTCC
TCCCGCACCCTGGACTTCGAGTCCGCGTTCCGTGCCGCTTCCGGCGGGGCGGGCATGGAC
GTCGTACTGAACTCGCTCGCCCGCGAGTTCGTCGACGCCTCGCTGCGCCTGCTCGGGCCG
GGCGGCCGGTTCGTGGAGATGGGGAAGACCGACGTCCGCGACGCGGAGCGGGTCGCCGCC
GACCACCCCGGTGTCGGCTACCGCGCCTTCGACCTGGGCGAGGCCGGGCCGGAGCGGATC
GGCGAGATGCTCGCCGAGGTCATCGCCCTCTTCGAGGACGGGGTGCTCCGGCACCTGCCC
GTCACGACCTGGGACGTGCGCCGGGCCCGCGACGCCTTCCGGCACGTCAGCCAGGCCCGC
CACACGGGCAAGGTCGTCCTCACGATGCCGTCGGGCCTCGACCCGGAGGGTACGGTCCTG
CTGACCGGCGGCACCGGTGCGCTGGGGGGCATCGTGGCCCGGCACGTGGTGGGCGAGTGG
GGCGTACGACGCCTGCTGCTCGTGAGCCGGCGGGGCACGGACGCCCCGGGCGCCGGCGAG
CTCGTGCACGAGCTGGAGGCCCTGGGAGCCGACGTCTCGGTGGCCGCGTGCGACGTCGCC
GACCGCGAAGCCCTCACCGCCGTACTCGACTCGATCCCCGCCGAACACCCGCTCACCGCG
GTCGTCCACACGGCAGGCGTCCTCTCCGACGGCACCCTCCCCTCGATGACAGCGGAGGAT
GTGGAACACGTACTGCGTCCCAAGGTCGACGCCGCGTTCCTCCTCGACGAACTCACCTCG
ACGCCCGGCTACGACCTGGCAGCGTTCGTCATGTTCTCCTCCGCCGCCGCCGTCTTCGGT
GGCGCGGGGCAGGGCGCCTACGCCGCCGCCAACGCCACCCTCGACGCCCTCGCCTGGCGC
CGCCGGACAGCCGGACTCCCCGCCCTCTCCCTCGGCTGGGGCCTCTGGGCCGAGACCAGC
GGCATGACCGGCGGACTCAGCGACACCGACCGCTCGCGGCTGGCCCGTTCCGGGGCGACG
CCCATGGACAGCGAGCTGACCCTGTCCCTCCTGGACGCGGCCATGCGCCGCGACGACCCG
GCGCTCGTCCCGATCGCCCTGGACGTCGCCGCGCTCCGCGCCCAGCAGCGCGACGGCATG
CTGGCGCCGCTGCTCAGCGGGCTCACCCGCGGATCGCGGGTCGGCGGCGCGCCGGTCAAC
CAGCGCAGGGCAGCCGCCGGAGGCGCGGGCGAGGCGGACACGGACCTCGGCGGGCGGCTC
GCCGCGATGACACCGGACGACCGGGTCGCGCACCTGCGGGACCTCGTCCGTACGCACGTG
GCGACCGTCCTGGGACACGGCACCCCGAGCCGGGTGGACCTGGAGCGGGCCTTCCGCGAC
ACCGGTTTCGACTCGCTCACCGCCGTCGAACTCCGCAACCGTCTCAACGCCGCGACCGGG
CTGCGGCTGCCGGCCACGCTGGTCTTCGACCACCCCACCCCGGGGGAGCTCGCCGGGCAC
CTGCTCGACGAACTCGCCACGGCCGCGGGCGGGTCCTGGGCGGAAGGCACCGGGTCCGGA
GACACGGCCTCGGCGACCGATCGGCAGACCACGGCGGCCCTCGCCGAACTCGACCGGCTG
GAAGGCGTGCTCGCCTCCCTCGCGCCCGCCGCCGGCGGCCGTCCGGAGCTCGCCGCCCGG
CTCAGGGCGCTGGCCGCGGCCCTGGGGGACGACGGCGACGACGCCACCGACCTGGACGAG
GCGTCCGACGACGACCTCTTCTCCTTCATCGACAAGGAGCTGGGCGACTCCGACTTCTGA
[3] KS36..412
[3] AT572..881
[3] methylmalonyl-CoA762..766
[3] kr1150..1345
[3] ACP1443..1517
[4] KS1539..1916
[4] AT2069..2379
[4] methylmalonyl-CoA2259..2263
[4] DH2428..2593
[4] ER2959..3267
[4] KR3277..3459
[4] ACP3572..3642
[3] KS106..1236
[3] AT1714..2643
[3] methylmalonyl-CoA2284..2298
[3] kr3448..4035
[3] ACP4327..4551
[4] KS4615..5748
[4] AT6205..7137
[4] methylmalonyl-CoA6775..6789
[4] DH7282..7779
[4] ER8875..9801
[4] KR9829..10377
[4] ACP10714..10926

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001227 Acyl transferase domain (Domain)
[561-694] G3DSA:3.40.366.10 [762-872] G3DSA:3.40.366.10 [2062-2191] G3DSA:3.40.366.10 [2259-2369] G3DSA:3.40.366.10
G3DSA:3.40.366.10   Ac_transferase_reg
IPR002364 Quinone oxidoreductase/zeta-crystallin, conserved site (Conserved_site)
[3089-3110] PS01162
PS01162   QOR_ZETA_CRYSTAL
IPR006162 Phosphopantetheine attachment site (PTM)
[1475-1490] PS00012 [3600-3615] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[1443-1517] PS50075 [3572-3642] PS50075
PS50075   ACP_DOMAIN
[1444-1520] 6.00000000000001e-50 G3DSA:1.10.1200.10 [3571-3646] 6.00000000000001e-50 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1440-1556] 1.29999924468179e-27 SSF47336 [3564-3659] 6.39998633403535e-24 SSF47336
SSF47336   ACP_like
[1450-1516] 5.8e-12 PF00550 [3575-3641] 1.9e-13 PF00550
PF00550   PP-binding
IPR011032 GroES-like (Domain)
[2972-3107] 5.7999965385478e-34 SSF50129
SSF50129   GroES_like
IPR013149 Alcohol dehydrogenase, C-terminal (Domain)
[3100-3191] 4.3e-16 PF00107
PF00107   ADH_zinc_N
IPR013154 Alcohol dehydrogenase GroES-like (Domain)
[2981-3036] 9.7e-09 PF08240
PF08240   ADH_N
IPR013968 Polyketide synthase, KR (Domain)
[1150-1344] 1.09999999999999e-45 PF08659 [3277-3458] 4.2e-57 PF08659
PF08659   KR
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[36-286] 1.10000000000001e-96 PF00109 [1539-1790] 2.59999999999995e-100 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[294-412] 1.6e-48 PF02801 [1798-1916] 3.80000000000003e-49 PF02801
PF02801   Ketoacyl-synt_C
IPR014043 Acyl transferase (Domain)
[572-881] 2.70000000000002e-99 PF00698 [2069-2379] 2.59999999999995e-100 PF00698
PF00698   Acyl_transf_1
IPR015083 Polyketide synthase, docking (Domain)
[4-29] 5.59999999999999e-11 PF08990
PF08990   Docking
[6-36] 1.9000006847787e-06 SSF101173
SSF101173   Polyketide_synth_docking
IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase (Domain)
[569-861] 4.50001022902825e-62 SSF52151 [2066-2379] 4.10001399699315e-62 SSF52151
SSF52151   Acyl_Trfase/lysoPlipase
IPR016036 Malonyl-CoA ACP transacylase, ACP-binding (Domain)
[697-761] 9.59999914716171e-17 SSF55048 [2194-2258] 3.69999438558106e-17 SSF55048
SSF55048   Malonyl_transacylase_ACP-bd
IPR016038 Thiolase-like, subgroup (Domain)
[38-298] G3DSA:3.40.47.10 [299-466] 5.90000000000008e-125 G3DSA:3.40.47.10 [1542-1802] G3DSA:3.40.47.10 [1803-1969] 5.90000000000008e-125 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[28-466] 1.69998802370953e-103 SSF53901 [1539-1967] 6.40004254961849e-106 SSF53901
SSF53901   Thiolase-like
IPR016040 NAD(P)-binding domain (Domain)
[1148-1351] 6e-57 G3DSA:3.40.50.720 [3052-3241] 3.40000000000004e-48 G3DSA:3.40.50.720 [3277-3462] 1.70000000000001e-50 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[199-215] PS00606 [1703-1719] PS00606
PS00606   B_KETOACYL_SYNTHASE
IPR020801 Polyketide synthase, acyl transferase domain (Domain)
[573-864] 1.59998835313644e-114 SM00827 [2070-2361] 3.30001976117415e-118 SM00827
SM00827   PKS_AT
IPR020806 Polyketide synthase, phosphopantetheine-binding domain (Domain)
[1448-1520] 2.90000354677981e-36 SM00823 [3573-3645] 6.60002751141562e-38 SM00823
SM00823   PKS_PP
IPR020807 Polyketide synthase, dehydratase domain (Domain)
[2428-2593] 1.69998802370953e-92 SM00826
SM00826   PKS_DH
IPR020841 Polyketide synthase, beta-ketoacyl synthase domain (Domain)
[38-466] SM00825 [1542-1970] SM00825
SM00825   PKS_KS
IPR020842 Polyketide synthase/Fatty acid synthase, KR (Domain)
[1150-1345] 4.90001027782405e-20 SM00822 [3277-3459] 1.10000150671643e-55 SM00822
SM00822   PKS_KR
IPR020843 Polyketide synthase, enoylreductase (Domain)
[2959-3267] SM00829
SM00829   PKS_ER
SignalP No significant hit
TMHMM No significant hit
Pikro_00030 Pikro_00030   Pikro_00050 Pikro_00050
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