Pikro_00160 : CDS information

Location

Organism	Streptomyces venezuelae
Strain	ATCC 15439
Entry name	Pikromycin
Contig	AF079762
Start / Stop / Direction	51,944 / 50,697 / - [in whole cluster] 12,196 / 10,949 / - [in contig]
Location	complement(50697..51944) [in whole cluster] complement(10949..12196) [in contig]
Type	CDS
Length	1,248 bp (415 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose C-4 aminotransferase
Product (GenBank)	4-dehydrase
Gene
Gene (GenBank)	desI
EC number	2.6.1.-
Keyword	desosamine
Note
Note (GenBank)
Reference	ACC Q9ZGH0 PmId [9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998) [17630700] Molecular architecture of DesI: a key enzyme in the biosynthesis of desosamine. (Biochemistry. , 2007) comment [PMID: 9770448](1998) 12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。 DesI: 4-Dehydrase --- [PMID: 17630700](2007) PLP-dependent aminotransferase_DesIのX線結晶構造解析。 DesIをその反応産物であるdTDP-4-amino-4,6-dideoxyglucoseとの複合体で解析。 DesIと同様に産物のdideoxyglucosyl基とタンパクとの間の水素結合相互作用がないHelicobacter pylori_PseCとの構造比較から、転移する糖の向きの違いを説明している。
Related Reference	PmId [16514147] In vivo characterization of the dTDP-D-desosamine pathway of the megalomicin gene cluster from Micromonospora megalomicea. (Microbiology. , 2006) comment megalomicin生合成gene clusterにおいて、megosamine生合成経路は実験的に確認され、それまでの経路は再構築されている。

Sequence

>putative NDP-hexose C-4 aminotransferase [4-dehydrase]
MKSALSDLAFFGGPAAFDQPLLVGRPNRIDRARLYERLDRALDSQWLSNGGPLVREFEER
VAGLAGVRHAVATCNATAGLQLLAHAAGLTGEVIMPSMTFAATPHALRWIGLTPVFADID
PDTGNLDPDQVAAAVTPRTSAVVGVHLWGRPCAADQLRKVADEHGLRLYFDAAHALGCAV
DGRPAGSLGDAEVFSFHATKAVNAFEGGAVVTDDADLAARIRALHNFGFDLPGGSPAGGT
NAKMSEAAAAMGLTSLDAFPEVIDRNRRNHAAYREHLADLPGVLVADHDRHGLNNHQYVI
VEIDEATTGIHRDLVMEVLKAEGVHTRAYFSPGCHELEPYRGQPHAPLPHTERLAARVLS
LPTGTAIGDDDIRRVADLLRLCATRGRELTARHRDTAPAPLAAPQTSTPTIGRSR

>putative NDP-hexose C-4 aminotransferase [4-dehydrase]
GTGAAAAGCGCCTTATCCGACCTCGCATTCTTCGGCGGCCCCGCCGCTTTCGACCAGCCG
CTCCTCGTGGGGCGGCCCAACCGCATCGACCGCGCCAGGCTGTACGAGCGGCTCGACCGG
GCCCTCGACAGCCAGTGGCTGTCCAACGGCGGCCCGCTCGTCCGCGAGTTCGAGGAGCGC
GTCGCCGGGCTCGCCGGGGTCCGGCATGCCGTGGCCACCTGCAACGCCACGGCCGGGCTC
CAGCTCCTCGCGCACGCCGCCGGCCTCACCGGCGAAGTGATCATGCCGTCGATGACGTTC
GCCGCCACCCCGCACGCACTGCGCTGGATCGGCCTCACCCCGGTCTTCGCCGACATCGAC
CCGGACACCGGCAACCTCGACCCGGACCAGGTGGCCGCCGCGGTCACACCCCGCACCTCG
GCCGTCGTCGGCGTCCACCTCTGGGGCCGCCCCTGCGCCGCCGACCAGCTGCGGAAGGTC
GCCGACGAGCACGGCCTGCGGCTGTACTTCGACGCCGCGCACGCCCTCGGCTGCGCGGTC
GACGGCCGGCCCGCCGGCAGCCTCGGCGACGCCGAGGTCTTCAGCTTCCACGCCACCAAG
GCCGTCAACGCCTTCGAGGGCGGCGCCGTCGTCACCGACGACGCCGACCTCGCCGCCCGG
ATCCGCGCCCTCCACAACTTCGGCTTCGACCTGCCCGGCGGCAGCCCCGCCGGCGGGACC
AACGCCAAGATGAGCGAGGCCGCCGCCGCCATGGGCCTCACCTCCCTCGACGCGTTTCCC
GAGGTCATCGACCGGAACCGGCGCAACCACGCCGCCTACCGCGAGCACCTCGCGGACCTC
CCCGGCGTCCTCGTCGCCGACCACGACCGCCACGGCCTCAACAACCACCAGTACGTGATC
GTCGAGATCGACGAGGCCACCACCGGCATCCACCGCGACCTCGTCATGGAGGTCCTGAAG
GCCGAAGGCGTGCACACCCGCGCCTACTTCTCGCCGGGCTGCCACGAGCTGGAGCCGTAC
CGCGGGCAGCCGCACGCCCCGCTGCCGCACACCGAACGCCTCGCCGCGCGCGTGCTGTCC
CTGCCGACCGGCACCGCCATCGGCGACGACGACATCCGCCGGGTCGCCGACCTGCTGCGT
CTCTGCGCGACCCGCGGCCGCGAACTGACCGCGCGCCACCGCGACACGGCCCCCGCCCCG
CTCGCGGCCCCCCAGACATCCACGCCCACGATTGGACGCTCCCGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR000653 DegT/DnrJ/EryC1/StrS aminotransferase (Family) PF01041 DegT_DnrJ_EryC1 PIRSF000390 PLP_StrS IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain) G3DSA:3.40.640.10 PyrdxlP-dep_Trfase_major_sub1 IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain) G3DSA:3.90.1150.10 PyrdxlP-dep_Trfase_major_sub2 IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain) SSF53383 PyrdxlP-dep_Trfase_major
SignalP	No significant hit
TMHMM	No significant hit

Pikro_00150 Pikro_00170