Pikro_00150 : CDS information

Location

Organism	Streptomyces venezuelae
Strain	ATCC 15439
Entry name	Pikromycin
Contig	AF079762
Start / Stop / Direction	50,700 / 49,243 / - [in whole cluster] 10,952 / 9,495 / - [in contig]
Location	complement(49243..50700) [in whole cluster] complement(9495..10952) [in contig]
Type	CDS
Length	1,458 bp (485 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	SAM-dependent NDP-hexose C-4 deaminase/C-3 dehydrogenase
Product (GenBank)	putative reductase
Gene
Gene (GenBank)	desII
EC number
Keyword	desosamine
Note	This dehydrogenation function is not involved in desosamine biosynthesis.
Note (GenBank)
Reference	ACC Q9ZGH1 PmId [9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998) [19746907] Characterization and mechanistic studies of DesII: a radical S-adenosyl-L-methionine enzyme involved in the biosynthesis of TDP-D-desosamine. (J Am Chem Soc. , 2009) [20121093] Stoichiometry of the redox neutral deamination and oxidative dehydrogenation reactions catalyzed by the radical SAM enzyme DesII. (J Am Chem Soc. , 2010) [23329328] EPR-kinetic isotope effect study of the mechanism of radical-mediated dehydrogenation of an alcohol by the radical SAM enzyme DesII. (Proc Natl Acad Sci U S A. , 2013) [25826575] Mechanistic Investigation of the Radical S-Adenosyl-L-methionine Enzyme DesII Using Fluorinated Analogues. (J Am Chem Soc. , 2015) comment [PMID: 9770448](1998) 12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。 DesII: Reductase? --- [PMID: 19746907](2009) desosamine生合成において、DesIIはdTDP-4-amino-4,6-dideoxy-D-glucose → dTDP-3-keto-4,6-dideoxy-D-glucose への変換を担う。 DesIIはradical S-adenosyl-L-methionine(SAM) enzyme superfamilyのメンバーである。いくつかのhexoseのC-3, C-4での反応を触媒でき、C-4でのamino基はredox neutral deamination reactionを、C-4でのhydroxyl基の存在はC-3でのdehydrogenationを導く。C-4 hydroxyl基がaxialだと基質にならない。 DesII反応産物のHPLC解析、DesII reactionのsteady state kinetic parameters測定あり。 ↓この続報あり [PMID: 20121093](2010) [PMID: 23329328](2013) [PMID: 25826575](2015)

Sequence

>SAM-dependent NDP-hexose C-4 deaminase/C-3 dehydrogenase [putative reductase]
MTAPALSATAPAERCAHPGADLGAAVHAVGQTLAAGGLVPPDEAGTTARHLVRLAVRYGN
SPFTPLEEARHDLGVDRDAFRRLLALFGQVPELRTAVETGPAGAYWKNTLLPLEQRGVFD
AALARKPVFPYSVGLYPGPTCMFRCHFCVRVTGARYDPSALDAGNAMFRSVIDEIPAGNP
SAMYFSGGLEPLTNPGLGSLAAHATDHGLRPTVYTNSFALTERTLERQPGLWGLHAIRTS
LYGLNDEEYEQTTGKKAAFRRVRENLRRFQQLRAERESPINLGFAYIVLPGRASRLLDLV
DFIADLNDAGQGRTIDFVNIREDYSGRDDGKLPQEERAELQEALNAFEERVRERTPGLHI
DYGYALNSLRTGADAELLRIKPATMRPTAHPQVAVQVDLLGDVYLYREAGFPDLDGATRY
IAGRVTPDTSLTEVVRDFVERGGEVAAVDGDEYFMDGFDQVVTARLNQLERDAADGWEEA
RGFLR

>SAM-dependent NDP-hexose C-4 deaminase/C-3 dehydrogenase [putative reductase]
ATGACCGCCCCCGCCCTTTCCGCCACCGCCCCGGCCGAACGCTGCGCGCACCCCGGAGCC
GATCTGGGGGCGGCGGTCCACGCCGTCGGCCAGACCCTCGCCGCCGGCGGCCTCGTGCCG
CCCGACGAGGCCGGAACGACCGCCCGCCACCTCGTCCGGCTCGCCGTGCGCTACGGCAAC
AGCCCCTTCACCCCGCTGGAGGAGGCCCGCCACGACCTGGGCGTCGACCGGGACGCCTTC
CGGCGCCTCCTCGCCCTGTTCGGGCAGGTCCCGGAGCTCCGCACCGCGGTCGAGACCGGC
CCCGCCGGGGCGTACTGGAAGAACACCCTGCTCCCGCTCGAACAGCGCGGCGTCTTCGAC
GCGGCGCTCGCCAGGAAGCCCGTCTTCCCGTACAGCGTCGGCCTCTACCCCGGCCCGACC
TGCATGTTCCGCTGCCACTTCTGCGTCCGTGTGACCGGCGCCCGCTACGACCCGTCCGCC
CTCGACGCCGGCAACGCCATGTTCCGGTCGGTCATCGACGAGATACCCGCGGGCAACCCC
TCGGCGATGTACTTCTCCGGCGGCCTGGAGCCGCTCACCAACCCCGGCCTCGGGAGCCTG
GCCGCGCACGCCACCGACCACGGCCTGCGGCCCACCGTCTACACGAACTCCTTCGCGCTC
ACCGAGCGCACCCTGGAGCGCCAGCCCGGCCTCTGGGGCCTGCACGCCATCCGCACCTCG
CTCTACGGCCTCAACGACGAGGAGTACGAGCAGACCACCGGCAAGAAGGCCGCCTTCCGC
CGCGTCCGCGAGAACCTGCGCCGCTTCCAGCAGCTGCGCGCCGAGCGCGAGTCGCCGATC
AACCTCGGCTTCGCCTACATCGTGCTCCCGGGCCGTGCCTCCCGCCTGCTCGACCTGGTC
GACTTCATCGCCGACCTCAACGACGCCGGGCAGGGCAGGACGATCGACTTCGTCAACATT
CGCGAGGACTACAGCGGCCGTGACGACGGCAAGCTGCCGCAGGAGGAGCGGGCCGAGCTC
CAGGAGGCCCTCAACGCCTTCGAGGAGCGGGTCCGCGAGCGCACCCCCGGACTCCACATC
GACTACGGCTACGCCCTGAACAGCCTGCGCACCGGGGCCGACGCCGAACTGCTGCGGATC
AAGCCCGCCACCATGCGGCCCACCGCGCACCCGCAGGTCGCGGTGCAGGTCGATCTCCTC
GGCGACGTGTACCTGTACCGCGAGGCCGGCTTCCCCGACCTGGACGGCGCGACCCGCTAC
ATCGCGGGCCGCGTGACCCCCGACACCTCCCTCACCGAGGTCGTCAGGGACTTCGTCGAG
CGCGGCGGCGAGGTGGCGGCCGTCGACGGCGACGAGTACTTCATGGACGGCTTCGATCAG
GTCGTCACCGCCCGCCTGAACCAGCTGGAGCGCGACGCCGCGGACGGCTGGGAGGAGGCC
CGCGGCTTCCTGCGCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR016863 Reductase, EryCV, predicted (Family) PIRSF027982 Reductase_EryCV_prd
SignalP	No significant hit
TMHMM	No significant hit

Pikro_00140 Pikro_00160