Pikro_00140 : CDS information

Location

Organism	Streptomyces venezuelae
Strain	ATCC 15439
Entry name	Pikromycin
Contig	AF079762
Start / Stop / Direction	49,050 / 48,172 / - [in whole cluster] 9,302 / 8,424 / - [in contig]
Location	complement(48172..49050) [in whole cluster] complement(8424..9302) [in contig]
Type	CDS
Length	879 bp (292 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative glucose-1-phosphate thymidylyltransferase putative dTDP-glucose synthase
Product (GenBank)	alpha-D-glucose-1-phosphate thymidylyltransferase
Gene
Gene (GenBank)	desIII
EC number	2.7.7.24
Keyword	desosamine
Note
Note (GenBank)
Reference	ACC Q9ZGH2 PmId [9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998) comment [PMID: 9770448](1998) 12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。 DesIII: alpha-D-Glucose-1-phosphate thymidylyltransferase desI, desII, desIII, desIV, desV, desVI, and desVIII はdeoxysugar生合成を担う。
Related Reference	ACC Q9AGY4 PmId [12147463] Homologs of the Rml enzymes from Salmonella enterica are responsible for dTDP-beta-L-rhamnose biosynthesis in the gram-positive thermophile Aneurinibacillus thermoaerophilus DSM 10155. (Appl Environ Microbiol. , 2002) comment 59th(Q9AGY4) 60%, 6e-98 Aneurinibacillus thermoaerophilus_rmlA Glucose-1-phosphate thymidylyltransferase(EC 2.7.7.24) 活性測定あり

Sequence

>putative glucose-1-phosphate thymidylyltransferase [alpha-D-glucose-1-phosphate thymidylyltransferase]
MKGIVLAGGSGTRLHPATSVISKQILPVYNKPMIYYPLSVLMLGGIREIQIISTPQHIEL
FQSLLGNGRHLGIELDYAVQKEPAGIADALLVGAEHIGDDTCALILGDNIFHGPGLYTLL
RDSIARLDGCVLFGYPVKDPERYGVAEVDATGRLTDLVEKPVKPRSNLAVTGLYLYDNDV
VDIAKNIRPSPRGELEITDVNRVYLERGRAELVNLGRGFAWLDTGTHDSLLRAAQYVQVL
EERQGVWIAGLEEIAFRMGFIDAEACHGLGEGLSRTEYGSYLMEIAGREGAP

>putative glucose-1-phosphate thymidylyltransferase [alpha-D-glucose-1-phosphate thymidylyltransferase]
ATGAAGGGAATAGTCCTGGCCGGCGGGAGCGGAACTCGGCTGCATCCGGCGACCTCGGTC
ATTTCGAAGCAGATTCTTCCGGTCTACAACAAACCGATGATCTACTATCCGCTGTCGGTT
CTCATGCTCGGCGGTATTCGCGAGATTCAAATCATCTCGACCCCCCAGCACATCGAACTC
TTCCAGTCGCTTCTCGGAAACGGCAGGCACCTGGGAATAGAACTCGACTATGCGGTCCAG
AAAGAGCCCGCAGGAATCGCGGACGCACTTCTCGTCGGAGCCGAGCACATCGGCGACGAC
ACCTGCGCCCTGATCCTGGGCGACAACATCTTCCACGGGCCCGGCCTCTACACGCTCCTG
CGGGACAGCATCGCGCGCCTCGACGGCTGCGTGCTCTTCGGCTACCCGGTCAAGGACCCC
GAGCGGTACGGCGTCGCCGAGGTGGACGCGACGGGCCGGCTGACCGACCTCGTCGAGAAG
CCCGTCAAGCCGCGCTCCAACCTCGCCGTCACCGGCCTCTACCTCTACGACAACGACGTC
GTCGACATCGCCAAGAACATCCGGCCCTCGCCGCGCGGCGAGCTGGAGATCACCGACGTC
AACCGCGTCTACCTGGAGCGGGGCCGGGCCGAACTCGTCAACCTGGGCCGCGGCTTCGCC
TGGCTGGACACCGGCACCCACGACTCGCTCCTGCGGGCCGCCCAGTACGTCCAGGTCCTG
GAGGAGCGGCAGGGCGTCTGGATCGCGGGCCTTGAGGAGATCGCCTTCCGCATGGGCTTC
ATCGACGCCGAGGCCTGTCACGGCCTGGGAGAAGGCCTCTCCCGCACCGAGTACGGCAGC
TATCTGATGGAGATCGCCGGCCGCGAGGGAGCCCCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005835 Nucleotidyl transferase (Domain) PF00483 NTP_transferase IPR005907 Glucose-1-phosphate thymidylyltransferase, short form (Family) TIGR01207 RmlA
SignalP	No significant hit
TMHMM	No significant hit

Pikro_00130 Pikro_00150