Pikro_00120 Pikro_00120   Pikro_00140 Pikro_00140

Pikro_00130 : CDS information

close this sectionLocation

Organism
StrainATCC 15439
Entry namePikromycin
Contig
Start / Stop / Direction48,040 / 47,027 / - [in whole cluster]
8,292 / 7,279 / - [in contig]
Locationcomplement(47027..48040) [in whole cluster]
complement(7279..8292) [in contig]
TypeCDS
Length1,014 bp (337 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductdTDP-glucose 4,6-dehydratase
Product (GenBank)TDP-glucose-4,6-dehydratase
Gene
Gene (GenBank)desIV
EC number4.2.1.46
Keyword
  • desosamine
Note
Note (GenBank)
Reference
ACC
PmId
[9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998)
[14570895] High resolution X-ray structure of dTDP-glucose 4,6-dehydratase from Streptomyces venezuelae. (J Biol Chem. , 2004)
comment
[PMID: 9770448](1998)
12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。

DesIV: TDP-glucose 4,6-dehydratase
dTDP-glucose → dTDP-4-keto-6-deoxyglucose

desI, desII, desIII, desIV, desV, desVI, and desVIII はdeoxysugar生合成を担う。

---
[PMID: 14570895](2004)
DesIVの反応産物dTDP-4-keto-6-deoxy-D-glucoseを測定することで酵素活性をみて、activeなDesIV proteinのみを結晶化に使用している。

close this sectionSequence

selected fasta
>dTDP-glucose 4,6-dehydratase [TDP-glucose-4,6-dehydratase]
MRLLVTGGAGFIGSHFVRQLLAGAYPDVPADEVIVLDSLTYAGNRANLAPVDADPRLRFV
HGDIRDAGLLARELRGVDAIVHFAAESHVDRSIAGASVFTETNVQGTQTLLQCAVDAGVG
RVVHVSTDEVYGSIDSGSWTESSPLEPNSPYAASKAGSDLVARAYHRTYGLDVRITRCCN
NYGPYQHPEKLIPLFVTNLLDGGTLPLYGDGANVREWVHTDDHCRGIALVLAGGRAGEIY
HIGGGLELTNRELTGILLDSLGADWSSVRKVADRKGHDLRYSLDGGEIERELGYRPQVSF
ADGLARTVRWYRENRGWWEPLKATAPQLPATAVEVSA
selected fasta
>dTDP-glucose 4,6-dehydratase [TDP-glucose-4,6-dehydratase]
GTGCGGCTTCTGGTGACCGGAGGTGCGGGCTTCATCGGCTCGCACTTCGTGCGGCAGCTC
CTCGCCGGGGCGTACCCCGACGTGCCCGCCGATGAGGTGATCGTCCTGGACAGCCTCACC
TACGCGGGCAACCGCGCCAACCTCGCCCCGGTGGACGCGGACCCGCGACTGCGCTTCGTC
CACGGCGACATCCGCGACGCCGGCCTCCTCGCCCGGGAACTGCGCGGCGTGGACGCCATC
GTCCACTTCGCGGCCGAGAGCCACGTGGACCGCTCCATCGCGGGCGCGTCCGTGTTCACC
GAGACCAACGTGCAGGGCACGCAGACGCTGCTCCAGTGCGCCGTCGACGCCGGCGTCGGC
CGGGTCGTGCACGTCTCCACCGACGAGGTGTACGGGTCGATCGACTCCGGCTCCTGGACC
GAGAGCAGCCCGCTGGAGCCCAACTCGCCCTACGCGGCGTCCAAGGCCGGCTCCGACCTC
GTTGCCCGCGCCTACCACCGGACGTACGGCCTCGACGTACGGATCACCCGCTGCTGCAAC
AACTACGGGCCGTACCAGCACCCCGAGAAGCTCATCCCCCTCTTCGTGACGAACCTCCTC
GACGGCGGGACGCTCCCGCTGTACGGCGACGGCGCGAACGTCCGCGAGTGGGTGCACACC
GACGACCACTGCCGGGGCATCGCGCTCGTCCTCGCGGGCGGCCGGGCCGGCGAGATCTAC
CACATCGGCGGCGGCCTGGAGCTGACCAACCGCGAACTCACCGGCATCCTCCTGGACTCG
CTCGGCGCCGACTGGTCCTCGGTCCGGAAGGTCGCCGACCGCAAGGGCCACGACCTGCGC
TACTCCCTCGACGGCGGCGAGATCGAGCGCGAGCTCGGCTACCGCCCGCAGGTCTCCTTC
GCGGACGGCCTCGCGCGGACCGTCCGCTGGTACCGGGAGAACCGCGGCTGGTGGGAGCCG
CTCAAGGCGACCGCCCCGCAGCTGCCCGCCACCGCCGTGGAGGTGTCCGCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
[4-243] 9.9e-65 PF01370
PF01370   Epimerase
IPR005888 dTDP-glucose 4,6-dehydratase (Family)
[2-321] 7.99999999999993e-127 TIGR01181
TIGR01181   DTDP_gluc_dehyt
IPR016040 NAD(P)-binding domain (Domain)
[1-199] 1.10000000000001e-65 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[138-166] PS00061
PS00061   ADH_SHORT
SignalP
[1-22] 0.798 Signal
Eukaryota   
TMHMM No significant hit
Pikro_00120 Pikro_00120   Pikro_00140 Pikro_00140
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