Pikro_00110 Pikro_00110   Pikro_00130 Pikro_00130

Pikro_00120 : CDS information

close this sectionLocation

Organism
StrainATCC 15439
Entry namePikromycin
Contig
Start / Stop / Direction47,030 / 45,891 / - [in whole cluster]
7,282 / 6,143 / - [in contig]
Locationcomplement(45891..47030) [in whole cluster]
complement(6143..7282) [in contig]
TypeCDS
Length1,140 bp (379 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative NDP-hexose C-3 aminotransferase
Product (GenBank)transaminase
Gene
Gene (GenBank)desV
EC number2.6.1.-
Keyword
  • desosamine
Note
Note (GenBank)
Reference
ACC
PmId
[9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998)
[17456741] Molecular architecture of DesV from Streptomyces venezuelae: a PLP-dependent transaminase involved in the biosynthesis of the unusual sugar desosamine. (Protein Sci. , 2007)
comment
[PMID: 9770448](1998)
12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。

DesV: Transaminase

desVのDeletion/replacement mutationにより、methymycin, neomethymycin, narbomycin, and pikromycinは非産生。10-deoxymethynolide and narbonolideを蓄積。

---
[PMID: 17456741](2007)
DesVの結晶構造解析。
同じくaminotransferaseであるDesIの構造解析論文と同じ著者らによる。
Related Reference
ACC
Q9F837
NITE
Megalo_00050
PmId
[16514147] In vivo characterization of the dTDP-D-desosamine pathway of the megalomicin gene cluster from Micromonospora megalomicea. (Microbiology. , 2006)
[20418422] TDP-L-megosamine biosynthesis pathway elucidation and megalomicin a production in Escherichia coli. (Appl Environ Microbiol. , 2010)
comment
294th(Q9F837) 47%, 1e-77
Micromonospora megalomicea subsp. nigra_megDII
TDP-3-keto-6-deoxyhexose 3-aminotransaminase
[DoBISCUIT]NDP-hexose C-3 aminotransferase

異なる遺伝子セットを含んだオペロンによってE.coliで産生されたdTDP-sugar中間体のLC/MS/MS解析。セットの組み合わせによりMegDII の機能は確立されている。

MegDII は、desosamineとmegosamine両方の生合成において3-aminotransferaseとして働く。
基質は各経路で少し異なる。

close this sectionSequence

selected fasta
>putative NDP-hexose C-3 aminotransferase [transaminase]
MSSRAETPRVPFLDLKAAYEELRAETDAAIARVLDSGRYLLGPELEGFEAEFAAYCETDH
AVGVNSGMDALQLALRGLGIGPGDEVIVPSHTYIASWLAVSATGATPVPVEPHEDHPTLD
PLLVEKAITPRTRALLPVHLYGHPADMDALRELADRHGLHIVEDAAQAHGARYRGRRIGA
GSSVAAFSFYPGKNLGCFGDGGAVVTGDPELAERLRMLRNYGSRQKYSHETKGTNSRLDE
MQAAVLRIRLAHLDSWNGRRSALAAEYLSGLAGLPGIGLPVTAPDTDPVWHLFTVRTERR
DELRSHLDARGIDTLTHYPVPVHLSPAYAGEAPPEGSLPRAESFARQVLSLPIGPHLERP
QALRVIDAVREWAERVDQA
selected fasta
>putative NDP-hexose C-3 aminotransferase [transaminase]
GTGAGCAGCCGCGCCGAGACCCCCCGCGTCCCCTTCCTCGACCTCAAGGCCGCCTACGAG
GAGCTCCGCGCGGAGACCGACGCCGCGATCGCCCGCGTCCTCGACTCGGGGCGCTACCTC
CTCGGACCCGAACTCGAAGGATTCGAGGCGGAGTTCGCCGCGTACTGCGAGACGGACCAC
GCCGTCGGCGTGAACAGCGGGATGGACGCCCTCCAGCTCGCCCTCCGCGGCCTCGGCATC
GGACCCGGGGACGAGGTGATCGTCCCCTCGCACACGTACATCGCCAGCTGGCTCGCGGTG
TCCGCCACCGGCGCGACCCCCGTGCCCGTCGAGCCGCACGAGGACCACCCCACCCTGGAC
CCGCTGCTCGTCGAGAAGGCGATCACCCCCCGCACCCGGGCGCTCCTCCCCGTCCACCTC
TACGGGCACCCCGCCGACATGGACGCCCTCCGCGAGCTCGCGGACCGGCACGGCCTGCAC
ATCGTCGAGGACGCCGCGCAGGCCCACGGCGCCCGCTACCGGGGCCGGCGGATCGGCGCC
GGGTCGTCGGTGGCCGCGTTCAGCTTCTACCCGGGCAAGAACCTCGGCTGCTTCGGCGAC
GGCGGCGCCGTCGTCACCGGCGACCCCGAGCTCGCCGAACGGCTCCGGATGCTCCGCAAC
TACGGCTCGCGGCAGAAGTACAGCCACGAGACGAAGGGCACCAACTCCCGCCTGGACGAG
ATGCAGGCCGCCGTGCTGCGGATCCGGCTCGCCCACCTGGACAGCTGGAACGGCCGCAGG
TCGGCGCTGGCCGCGGAGTACCTCTCCGGGCTCGCCGGACTGCCCGGCATCGGCCTGCCG
GTGACCGCGCCCGACACCGACCCGGTCTGGCACCTCTTCACCGTGCGCACCGAGCGCCGC
GACGAGCTGCGCAGCCACCTCGACGCCCGCGGCATCGACACCCTCACGCACTACCCGGTA
CCCGTGCACCTCTCGCCCGCCTACGCGGGCGAGGCACCGCCGGAAGGCTCGCTCCCGCGG
GCCGAGAGCTTCGCGCGGCAGGTCCTCAGCCTGCCGATCGGCCCGCACCTGGAGCGCCCG
CAGGCGCTGCGGGTGATCGACGCCGTGCGCGAATGGGCCGAGCGGGTCGACCAGGCCTAG

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000653 DegT/DnrJ/EryC1/StrS aminotransferase (Family)
[22-370] 3.60000000000002e-125 PF01041
PF01041   DegT_DnrJ_EryC1
[1-377] PIRSF000390
PIRSF000390   PLP_StrS
IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (Domain)
[10-251] 4.80000000000003e-91 G3DSA:3.40.640.10
G3DSA:3.40.640.10   PyrdxlP-dep_Trfase_major_sub1
IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (Domain)
[253-375] 5.10000000000004e-39 G3DSA:3.90.1150.10
G3DSA:3.90.1150.10   PyrdxlP-dep_Trfase_major_sub2
IPR015424 Pyridoxal phosphate-dependent transferase, major domain (Domain)
[6-376] 4.89996723796399e-112 SSF53383
SSF53383   PyrdxlP-dep_Trfase_major
SignalP No significant hit
TMHMM No significant hit
Pikro_00110 Pikro_00110   Pikro_00130 Pikro_00130
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