Pikro_00100 Pikro_00100   Pikro_00120 Pikro_00120

Pikro_00110 : CDS information

close this sectionLocation

Organism
StrainATCC 15439
Entry namePikromycin
Contig
Start / Stop / Direction43,373 / 45,802 / + [in whole cluster]
3,625 / 6,054 / + [in contig]
Location43373..45802 [in whole cluster]
3625..6054 [in contig]
TypeCDS
Length2,430 bp (809 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.4 resistance
Productbeta-glucosidase
Product (GenBank)beta-glucosidase
Gene
Gene (GenBank)desR
EC number3.2.1.21
Keyword
  • extracellular reactivation
Note
Note (GenBank)
  • putative extracellular protein involved in a resistance mechanism
Reference
ACC
PmId
[9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998)
[14674753] Beta-glucosylation as a part of self-resistance mechanism in methymycin/pikromycin producing strain Streptomyces venezuelae. (Biochemistry. , 2003)
comment
[PMID: 9770448](1998)
12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。

DesR: beta-Glucosidase (involved in resistance mechanism)

細菌の自己防衛のためのdrug inactivation-reactivation cycleに関連することが示されている。
Ref23←titleのみ, pubmedにない。
Biosynthesis of Desosamine: Molecular Evidence Suggesting beta-Glucosylation as a Self-Resistance Mechanism in Methymycin/Neomethymycin Producing Strain, Streptomyces venezuelae. J. Am. Chem. Soc./1998

---
[PMID: 14674753](2003)abstract
desRの破壊により、生物学的に不活性なglucosylated methymycin/neomethymycin productsが蓄積。
S. venezuelaeによって産生されるglucosylated methymycin/neomethymycinの加水分解できるbeta-glucosidaseとして、精製したDesRの触媒機能を確認。

desRは分泌タンパクに特徴的な配列を持つので、細胞外に輸送され、そこでglucosylated productsを活性化するために加水分解すると考えられる。

ただし、主要な自己抵抗性システムはPikR1 and PikR2による23S rRNAの修飾であり、このglycosylation/deglycosylationはS. venezuelaeにおいて二次的な自己防衛メカニズムである。
Related Reference
ACC
O68843
PmId
[9680207] Two glycosyltransferases and a glycosidase are involved in oleandomycin modification during its biosynthesis by Streptomyces antibioticus. (Mol Microbiol. , 1998)
comment
7th(O68843) 60%, 0.0
Streptomyces antibioticus_oleR
Glycosidase OleR

streptomycin生合成系の遺伝子strD, strE, strMをプローブとし、S. antibioticusゲノムライブラリーよりoleI, oleN2, oleRを単離し、シークエンス解析。
5'末より、strM-strD-strE-oleI-oleN2-oleR-PKS の順で並んでいる。
S.antibioticus_oleI, oleD, oleRそれぞれタンパク発現ベクターを構築し、S.lividansに形質転換・タンパク発現・酵素活性の確認。
-->oleIはoleDよりも高いoleandomycin　glycosylating活性あり
-->oleRにはoleandomycin　deglycosylating活性あり

oleRにより、glycosylated　oleandomycinから活性型oleandomycinに変換される。

close this sectionSequence

selected fasta
>beta-glucosidase [beta-glucosidase]
MTGKTRIPRVRRGRTTPRAFTLAVVGTLLAGTTVAAAAPGAADTANVQYTSRAAELVAQM
TLDEKISFVHWALDPDRQNVGYLPGVPRLGIPELRAADGPNGIRLVGQTATALPAPVALA
STFDDTMADSYGKVMGRDGRALNQDMVLGPMMNNIRVPHGGRNYETFSEDPLVSSRTAVA
QIKGIQGAGLMTTAKHFAANNQENNRFSVNANVDEQTLREIEFPAFEASSKAGAASFMCA
YNGLNGKPSCGNDELLNNVLRTQWGFQGWVMSDWLATPGTDAITKGLDQEMGVELPGDVP
KGEPSPPAKFFGEALKTAVLNGTVPEAAVTRSAERIVGQMEKFGLLLATPAPRPERDKAG
AQAVSRKVAENGAVLLRNEGQALPLAGDAGKSIAVIGPTAVDPKVTGLGSAHVVPDSAAA
PLDTIKARAGAGATVTYETGEETFGTQIPAGNLSPAFNQGHQLEPGKAGALYDGTLTVPA
DGEYRIAVRATGGYATVQLGSHTIEAGQVYGKVSSPLLKLTKGTHKLTISGFAMSATPLS
LELGWVTPAAADATIAKAVESARKARTAVVFAYDDGTEGVDRPNLSLPGTQDKLISAVAD
ANPNTIVVLNTGSSVLMPWLSKTRAVLDMWYPGQAGAEATAALLYGDVNPSGKLTQSFPA
AENQHAVAGDPTSYPGVDNQQTYREGIHVGYRWFDKENVKPLFPFGHGLSYTSFTQSAPT
VVRTSTGGLKVTVTVRNSGKRAGQEVVQAYLGASPNVTAPQAKKKLVGYTKVSLAAGEAK
TVTVNVDRRQLQTGSSSADLRGSATVNVW
selected fasta
>beta-glucosidase [beta-glucosidase]
GTGACAGGTAAGACCCGAATACCGCGTGTCCGCCGCGGCCGCACCACGCCCAGGGCCTTC
ACCCTGGCCGTCGTCGGCACCCTGCTGGCGGGCACCACCGTGGCGGCCGCCGCTCCCGGC
GCCGCCGACACGGCCAATGTTCAGTACACGAGCCGGGCGGCGGAGCTCGTCGCCCAGATG
ACGCTCGACGAGAAGATCAGCTTCGTCCACTGGGCGCTGGACCCCGACCGGCAGAACGTC
GGCTACCTTCCCGGCGTGCCGCGTCTGGGCATCCCGGAGCTGCGTGCCGCCGACGGCCCG
AACGGCATCCGCCTGGTGGGGCAGACCGCCACCGCGCTGCCCGCGCCGGTCGCCCTGGCC
AGCACCTTCGACGACACCATGGCCGACAGCTACGGCAAGGTCATGGGCCGCGACGGTCGC
GCGCTCAACCAGGACATGGTCCTGGGCCCGATGATGAACAACATCCGGGTGCCGCACGGC
GGCCGGAACTACGAGACCTTCAGCGAGGACCCCCTGGTCTCCTCGCGCACCGCGGTCGCC
CAGATCAAGGGCATCCAGGGTGCGGGTCTGATGACCACGGCCAAGCACTTCGCGGCCAAC
AACCAGGAGAACAACCGCTTCTCCGTGAACGCCAATGTCGACGAGCAGACGCTCCGCGAG
ATCGAGTTCCCGGCGTTCGAGGCGTCCTCCAAGGCCGGCGCGGCCTCCTTCATGTGTGCC
TACAACGGCCTCAACGGGAAGCCGTCCTGCGGCAACGACGAGCTCCTCAACAACGTGCTG
CGCACGCAGTGGGGCTTCCAGGGCTGGGTGATGTCCGACTGGCTCGCCACCCCGGGCACC
GACGCCATCACCAAGGGCCTCGACCAGGAGATGGGCGTCGAGCTCCCCGGCGACGTCCCG
AAGGGCGAGCCCTCGCCGCCGGCCAAGTTCTTCGGCGAGGCGCTGAAGACGGCCGTCCTG
AACGGCACGGTCCCCGAGGCGGCCGTGACGCGGTCGGCGGAGCGGATCGTCGGCCAGATG
GAGAAGTTCGGTCTGCTCCTCGCCACTCCGGCGCCGCGGCCCGAGCGCGACAAGGCGGGT
GCCCAGGCGGTGTCCCGCAAGGTCGCCGAGAACGGCGCGGTGCTCCTGCGCAACGAGGGC
CAGGCCCTGCCGCTCGCCGGTGACGCCGGCAAGAGCATCGCGGTCATCGGCCCGACGGCC
GTCGACCCCAAGGTCACCGGCCTGGGCAGCGCCCACGTCGTCCCGGACTCGGCGGCGGCG
CCACTCGACACCATCAAGGCCCGCGCGGGTGCGGGTGCGACGGTGACGTACGAGACGGGT
GAGGAGACCTTCGGGACGCAGATCCCGGCGGGGAACCTCAGCCCGGCGTTCAACCAGGGC
CACCAGCTCGAGCCGGGCAAGGCGGGGGCGCTGTACGACGGCACGCTGACCGTGCCCGCC
GACGGCGAGTACCGCATCGCGGTCCGTGCCACCGGTGGTTACGCCACGGTGCAGCTCGGC
AGCCACACCATCGAGGCCGGTCAGGTCTACGGCAAGGTGAGCAGCCCGCTCCTCAAGCTG
ACCAAGGGCACGCACAAGCTCACGATCTCGGGCTTCGCGATGAGTGCCACCCCGCTCTCC
CTGGAGCTGGGCTGGGTGACGCCGGCGGCGGCCGACGCGACGATCGCGAAGGCCGTGGAG
TCGGCGCGGAAGGCCCGTACGGCGGTCGTCTTCGCCTACGACGACGGCACCGAGGGCGTC
GACCGTCCGAACCTGTCGCTGCCGGGTACGCAGGACAAGCTGATCTCGGCTGTCGCGGAC
GCCAACCCGAACACGATCGTGGTCCTCAACACCGGTTCGTCGGTGCTGATGCCGTGGCTG
TCCAAGACCCGCGCGGTCCTGGACATGTGGTACCCGGGCCAGGCGGGCGCCGAGGCCACC
GCCGCGCTGCTCTACGGTGACGTCAACCCGAGCGGCAAGCTCACGCAGAGCTTCCCGGCC
GCCGAGAACCAGCACGCGGTCGCCGGCGACCCGACAAGCTACCCGGGCGTCGACAACCAG
CAGACGTACCGCGAGGGCATCCACGTCGGGTACCGCTGGTTCGACAAGGAGAACGTCAAG
CCGCTGTTCCCGTTCGGGCACGGCCTGTCGTACACCTCGTTCACGCAGAGCGCCCCGACC
GTCGTGCGTACGTCCACGGGTGGTCTGAAGGTCACGGTCACGGTCCGCAACAGCGGGAAG
CGCGCCGGCCAGGAGGTCGTCCAGGCGTACCTCGGTGCCAGCCCGAACGTGACGGCTCCG
CAGGCGAAGAAGAAGCTCGTGGGCTACACGAAGGTCTCGCTCGCCGCGGGCGAGGCGAAG
ACGGTGACGGTGAACGTCGACCGCCGTCAGCTGCAGACCGGTTCGTCCTCCGCCGACCTG
CGGGGCAGCGCCACGGTCAACGTCTGGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001764 Glycoside hydrolase, family 3, N-terminal (Domain)
[49-350] 7.79999999999999e-97 G3DSA:3.20.20.300
G3DSA:3.20.20.300   Glyco_hydro_3_N
[88-104] 3.89999861795218e-32 PR00133 [113-132] 3.89999861795218e-32 PR00133 [159-175] 3.89999861795218e-32 PR00133 [190-206] 3.89999861795218e-32 PR00133 [259-277] 3.89999861795218e-32 PR00133
PR00133   GLHYDRLASE3
[58-337] 5.80000000000004e-85 PF00933
PF00933   Glyco_hydro_3
IPR002772 Glycoside hydrolase family 3 C-terminal domain (Domain)
[361-442] 1.09999999999999e-62 G3DSA:3.40.50.1700 [557-714] 1.09999999999999e-62 G3DSA:3.40.50.1700
G3DSA:3.40.50.1700   G3DSA:3.40.50.1700
[373-691] 8.70000000000003e-61 PF01915
PF01915   Glyco_hydro_3_C
[373-714] 7.6999848053369e-50 SSF52279
SSF52279   Glyco_hydro_3_C
IPR011658 PA14 (Domain)
[429-558] 6.59999852526375e-25 SM00758
SM00758   PA14
[431-557] 6.19999999999999e-18 PF07691
PF07691   PA14
IPR017853 Glycoside hydrolase, superfamily (Domain)
[38-372] 2.2999842048857e-85 SSF51445
SSF51445   Glyco_hydro_cat
SignalP
[1-37] 1 Signal
Bacteria, Gram-negative   
[1-36] 0.971 Signal
Eukaryota   
[1-37] 1 Signal
Bacteria, Gram-positive   
TMHMM
[20-42] Transmembrane (i-o)
Transmembrane 1   
Pikro_00100 Pikro_00100   Pikro_00120 Pikro_00120
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