Pikro_00100 : CDS information

Location

Organism	Streptomyces venezuelae
Strain	ATCC 15439
Entry name	Pikromycin
Contig	AF079762
Start / Stop / Direction	42,593 / 43,306 / + [in whole cluster] 2,845 / 3,558 / + [in contig]
Location	42593..43306 [in whole cluster] 2845..3558 [in contig]
Type	CDS
Length	714 bp (237 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	NDP-hexose N,N-dimethyltransferase
Product (GenBank)	N,N-dimethyltransferase
Gene
Gene (GenBank)	desVI
EC number	2.1.1.-
Keyword	desosamine
Note
Note (GenBank)
Reference	ACC Q9ZGH6 PmId [9770448] A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity. (Proc Natl Acad Sci U S A. , 1998) [12119032] Expression, purification, and characterization of two N,N-dimethyltransferases, tylM1 and desVI, involved in the biosynthesis of mycaminose and desosamine. (Biochemistry. , 2002) comment [PMID: 9770448](1998) 12員環骨格を持つMethymycin and Neomethymycinと、14員環骨格を持つNarbomycin and Pikromycinの生合成で共有されるclusterの同定。 DesVI: N,N-Dimethyltransferase desI, desII, desIII, desIV, desV, desVI, and desVIII はdeoxysugar生合成を担う。 desVI のDeletion/replacement mutationにより、methymycin, neomethymycin, narbomycin, and pikromycinは非産生。それらのaglyconeである10-deoxymethynolide and narbonolideを蓄積。 --- [PMID: 12119032](2002)abstract N,N-dimethyltransferasesであるtylM1 and desVI の過剰発現、精製、触媒機能確認。 TylM1 and DesVI はhomodimeric proteinで、ほとんど同じ生化学的特性を持つ。
Related Reference	ACC Q9F836 NITE Megalo_00060 PmId [16514147] In vivo characterization of the dTDP-D-desosamine pathway of the megalomicin gene cluster from Micromonospora megalomicea. (Microbiology. , 2006) [20418422] TDP-L-megosamine biosynthesis pathway elucidation and megalomicin a production in Escherichia coli. (Appl Environ Microbiol. , 2010) comment 34th(Q9F836) 51%, 2e-60 Micromonospora megalomicea subsp. nigra_megDIII [Megalo_00060]NDP-hexose N,N-dimethyltransferase 異なる遺伝子セットを含んだオペロンによってE.coliで産生されたdTDP-sugar中間体のLC/MS/MS analysis。 megosamine生合成のみならず、desosamine生合成においてもMegDIIIが機能することがセットの組み合わせにより確立されている。

Sequence

>NDP-hexose N,N-dimethyltransferase [N,N-dimethyltransferase]
MYEVDHADVYDLFYLGRGKDYAAEASDIADLVRSRTPEASSLLDVACGTGTHLEHFTKEF
GDTAGLELSEDMLTHARKRLPDATLHQGDMRDFRLGRKFSAVVSMFSSVGYLKTTEELGA
AVASFAEHLEPGGVVVVEPWWFPETFADGWVSADVVRRDGRTVARVSHSVREGNATRMEV
HFTVADPGKGVRHFSDVHLITLFHQAEYEAAFTAAGLRVEYLEGGPSGRGLFVGVPA

>NDP-hexose N,N-dimethyltransferase [N,N-dimethyltransferase]
GTGTACGAAGTCGACCACGCCGACGTCTACGACCTCTTCTACCTGGGTCGCGGCAAGGAC
TACGCCGCCGAGGCCTCCGACATCGCCGACCTGGTGCGCTCCCGTACCCCCGAGGCCTCC
TCGCTCCTGGACGTGGCCTGCGGTACGGGCACGCATCTGGAGCACTTCACCAAGGAGTTC
GGCGACACCGCCGGCCTGGAGCTGTCCGAGGACATGCTCACCCACGCCCGCAAGCGGCTG
CCCGACGCCACGCTCCACCAGGGCGACATGCGGGACTTCCGGCTCGGCCGGAAGTTCTCC
GCCGTGGTCAGCATGTTCAGCTCCGTCGGCTACCTGAAGACGACCGAGGAACTCGGCGCG
GCCGTCGCCTCGTTCGCGGAGCACCTGGAGCCCGGTGGCGTCGTCGTCGTCGAGCCGTGG
TGGTTCCCGGAGACCTTCGCCGACGGCTGGGTCAGCGCCGACGTCGTCCGCCGTGACGGG
CGCACCGTGGCCCGTGTCTCGCACTCGGTGCGGGAGGGGAACGCGACGCGCATGGAGGTC
CACTTCACCGTGGCCGACCCGGGCAAGGGCGTGCGGCACTTCTCCGACGTCCATCTCATC
ACCCTGTTCCACCAGGCCGAGTACGAGGCCGCGTTCACGGCCGCCGGGCTGCGCGTCGAG
TACCTGGAGGGCGGCCCGTCGGGCCGTGGCCTCTTCGTCGGCGTCCCCGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	No significant hit
SignalP	Bacteria, Gram-negative Bacteria, Gram-positive
TMHMM	No significant hit

Pikro_00090 Pikro_00110