Aver_00110 : CDS information

Location

Organism	Streptomyces avermitilis
Strain	ATCC 31267 (=NBRC 14893)
Entry name	Avermectin
Contig	AB032523
Start / Stop / Direction	71,886 / 73,124 / + [in whole cluster] 931 / 2,169 / + [in contig]
Location	71886..73124 [in whole cluster] 931..2169 [in contig]
Type	CDS
Length	1,239 bp (412 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	glycosyltransferase
Product (GenBank)	glycosyl transferase
Gene
Gene (GenBank)	aveBI
EC number
Keyword
Note
Note (GenBank)	transfer of oleandrose onto polyketide-derived aglycons
Reference	ACC Q9S0P6 PmId [10449723] Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis. (Proc Natl Acad Sci U S A. , 1999) [11451669] Insights about the biosynthesis of the avermectin deoxysugar L-oleandrose through heterologous expression of Streptomyces avermitilis deoxysugar genes in Streptomyces lividans. (Chem Biol. , 2001) [17177349] The in vitro characterization of the iterative avermectin glycosyltransferase AveBI reveals reaction reversibility and sugar nucleotide flexibility. (J Am Chem Soc. , 2006) comment [PMID:10449723](1999) avermectin生合成gene clusterの報告。 AveBI: Glycosyl transferase 明らかにaglyconeへoleandroseを移動するglycosyltransferaseだとの記述があるが、この論文で実験しているわけではない。 --- [PMID: 11451669](2001) AveBI = AvrB S. lividansでS. avermitilis avrBCDEFGHI を発現させて外因性aglyconeと反応させるとavermectin産生可能だが、avrCDEFGHI (delta-avrB)だと不可能。 avrBを含みその他の修飾があるplasmidでは、L-oleandroseではなくL-olivoseとそのC3立体異性体L-digitoxose(A1a1, 2)やD-olivose(A1a3)などを糖鎖として持つavermectin誘導体が得られたことから、ある程度の柔軟性を持つglycosyltransferaseである。 D-olivose同士を相互接続できない。disaccharideの移動はできない。 --- [PMID:17177349](2006)abstract glycosyltransferase AveBI のin vitro characterization. AveBI はoleandroseの2つの分離した反復性付加を触媒する。また、可逆性にも反応する。糖ヌクレオチド、aglyconesともに柔軟な受け入れが可能。

Sequence

>glycosyltransferase [glycosyl transferase]
MSDHFLFMSAPFWGHVFPSLAVAEELVHRGHHVTFVTGAEMADAVRSVGADFLRYESAFE
GVDMYRLMTEAEPNAIPMTLYDEGMSMLRSVEEHVGKDVPDLVAYDIATSLNVGRVLAAS
WSRPAMTVIPLFASNGRFSTMQSVLDPDSAQVSAPPPRFSEQMELFGLGALVPRLAELLV
SRGITEPVDDFLSGPEDFNLVCLPRAFQYAGDTFDERFAFVGPCLGKRRGLGEWTPPGSG
HPVVLISLGTVFNRQLSFFRTFVRAFTDVPVHVVISLGKGVDPDVLRPLPPNVEVHRWVP
HHAVLEHARALVTHGGTGSVMEALHAGCPVLVMPLSRDAQVTGRRIAELGLGRMVQPEEV
TATTLRRHVLDIISDDAITRQVRQMQRATVEAGGALRAADETERFLRRTRRH

>glycosyltransferase [glycosyl transferase]
ATGTCAGATCATTTTCTCTTCATGAGTGCGCCGTTCTGGGGGCATGTGTTCCCCAGTCTC
GCCGTGGCGGAGGAGCTCGTGCACCGGGGCCACCACGTCACCTTTGTGACGGGCGCGGAA
ATGGCCGATGCGGTGCGTTCCGTGGGCGCTGATTTCCTGCGGTACGAGTCCGCCTTCGAG
GGTGTCGACATGTACCGGCTGATGACCGAGGCCGAGCCGAACGCCATCCCCATGACGCTG
TACGACGAGGGCATGTCCATGTTGCGTTCGGTGGAGGAGCACGTCGGCAAGGACGTTCCG
GACCTGGTGGCCTACGACATCGCCACCTCCCTCAACGTGGGTCGTGTCCTCGCCGCCTCC
TGGAGCAGGCCGGCCATGACGGTCATTCCCCTGTTCGCGTCCAACGGGCGCTTCTCCACG
ATGCAGTCGGTATTGGATCCGGATTCCGCTCAGGTCAGTGCGCCGCCGCCGCGCTTCTCG
GAGCAGATGGAGTTGTTCGGCCTCGGGGCGCTGGTGCCGCGCCTCGCGGAGCTGCTCGTT
TCCCGGGGTATCACGGAACCGGTCGACGATTTCCTTTCCGGACCGGAGGACTTCAACCTG
GTGTGTCTGCCGCGCGCCTTCCAGTACGCGGGCGACACCTTCGACGAGCGGTTCGCCTTC
GTCGGACCATGTCTGGGTAAGCGCAGGGGTCTGGGCGAGTGGACACCACCGGGCAGCGGG
CATCCAGTGGTGCTCATCTCCCTCGGGACCGTGTTCAACCGGCAGCTGTCCTTCTTCCGC
ACGTTCGTCCGGGCGTTCACCGACGTCCCCGTGCACGTCGTGATCTCGCTCGGCAAGGGG
GTCGACCCCGATGTGCTGCGGCCGCTGCCGCCGAATGTCGAGGTGCACCGGTGGGTGCCG
CACCATGCGGTGCTGGAGCATGCCAGGGCTCTGGTCACGCACGGCGGTACCGGCAGTGTG
ATGGAGGCACTGCACGCAGGGTGCCCGGTGCTCGTCATGCCCTTGTCGCGGGACGCGCAG
GTGACCGGCCGGCGGATCGCCGAGCTGGGGCTGGGTCGTATGGTGCAGCCGGAGGAGGTC
ACGGCGACGACGCTGCGCCGGCACGTGCTGGACATCATCTCCGATGACGCGATCACCCGA
CAGGTCAGGCAGATGCAGCGGGCCACGGTCGAGGCGGGCGGCGCCCTGCGGGCAGCGGAC
GAGACCGAGCGGTTTCTGCGCCGGACGCGCCGTCACTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002213 UDP-glucuronosyl/UDP-glucosyltransferase (Family) PF00201 UDPGT IPR004276 Glycosyl transferase, family 28 (Domain) PF03033 Glyco_transf_28 IPR006326 UDP-glycosyltransferase, MGT (Family) TIGR01426 MGT
SignalP	Bacteria, Gram-negative Eukaryota Bacteria, Gram-positive
TMHMM	No significant hit

Aver_00100 Aver_00120