Aver_00120 : CDS information

Location

Organism	Streptomyces avermitilis
Strain	ATCC 31267 (=NBRC 14893)
Entry name	Avermectin
Contig	AB032523
Start / Stop / Direction	74,109 / 73,030 / - [in whole cluster] 3,154 / 2,075 / - [in contig]
Location	complement(73030..74109) [in whole cluster] complement(2075..3154) [in contig]
Type	CDS
Length	1,080 bp (359 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative dTDP-glucose 4,6-dehydratase
Product (GenBank)	dTDP-glucose 4,6-dehydratase
Gene
Gene (GenBank)	aveBII
EC number	4.2.1.46
Keyword	L-oleandrose
Note	The N-terminal position was modified from original INSDC entry.
Note (GenBank)	function in oleandrose biosynthesis
Reference	ACC Q9S0P5 PmId [10449723] Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis. (Proc Natl Acad Sci U S A. , 1999) [11451669] Insights about the biosynthesis of the avermectin deoxysugar L-oleandrose through heterologous expression of Streptomyces avermitilis deoxysugar genes in Streptomyces lividans. (Chem Biol. , 2001) comment [PMID:10449723](1999) avermectin生合成gene clusterの報告。 AveBII: dTDP-glucose 4,6-dehydratase この論文で実験確認しているわけではない。 --- [PMID: 11451669](2001) S.lividansでS.avermitilis avrBCDEFGHIを発現させて外因性aglyconeと反応させて産物解析。 thymidylyl transferase (avrD/aveBIII)と、続くdehydratase (avrC/aveBII)の反応は、細菌における6-deoxyhexoses形成で共通のfirst 2stepsである。ということで特に実験的に確かめていはいない。
Related Reference	ACC Q54116 PmId [7883182] Cloning of the genes encoding thymidine diphosphoglucose 4,6-dehydratase and thymidine diphospho-4-keto-6-deoxyglucose 3,5-epimerase from the erythromycin-producing Saccharopolyspora erythraea. (Gene. , 1995) comment 7th(Q54116) 69%, 1e-132 Saccharopolyspora erythraea_gdh dTDP-glucose 4,6-dehydratase(EC 4.2.1.46) gdhをE.coliで発現、抽出、精製して活性確認。

Sequence

>putative dTDP-glucose 4,6-dehydratase [dTDP-glucose 4,6-dehydratase]
MRLLVTGGAGFIGSHFVRRLLTGAYPAFTGAEVVVLDKLTYAGRLENLAPVLGSPSLIFV
HGDICDGPLVADLMDGSDMVVHFAAESHVDRSVADAAEFVRTNVLGTHTLLRAATDAAVD
RFVYISTDEVYGSIDSGSWTEDAPLEPNSPYSASKASSDLLARSFHRTHGLPVIITRCSN
NYGPHQFPEKLIPRFVTHLLNGTKVPLYGDGENVRDWLHVDDHCRGIALVAERGRPGEIY
HIGGGTELSNRELTARLLDLLGVDWSMVEPVTDRKGHDRRYSLDISKISAELGYAPRVPF
EEGLAQTVQWYVENRTLWEPLTARPELPVSDGASGAETARSRPLPAGRRPPRPWPAASA

>putative dTDP-glucose 4,6-dehydratase [dTDP-glucose 4,6-dehydratase]
ATGCGCCTACTCGTGACGGGCGGCGCCGGTTTCATCGGTTCCCATTTCGTACGGCGGTTG
CTGACGGGCGCGTATCCGGCGTTCACCGGTGCCGAGGTCGTGGTGCTCGACAAGCTGACC
TACGCGGGTCGCCTGGAGAACCTCGCACCGGTCCTGGGAAGCCCATCCCTGATCTTCGTG
CACGGCGACATCTGCGACGGGCCCTTGGTCGCGGACCTGATGGACGGCTCCGACATGGTG
GTCCACTTCGCGGCGGAGTCACACGTGGACCGGTCCGTGGCCGACGCCGCCGAGTTCGTA
CGCACCAATGTCCTCGGCACTCACACCCTGCTCCGCGCCGCCACCGACGCCGCCGTGGAC
CGCTTCGTGTACATCTCGACGGACGAGGTCTACGGCTCCATCGACTCCGGCTCGTGGACC
GAGGACGCCCCGCTGGAGCCGAACTCGCCCTACTCCGCGTCCAAGGCGTCCTCCGACCTG
CTCGCCCGGTCCTTCCACCGCACGCACGGCCTACCAGTGATCATCACCCGATGCTCGAAC
AACTACGGCCCCCACCAGTTCCCGGAGAAACTCATCCCGCGGTTCGTCACCCATCTGCTG
AACGGCACGAAGGTGCCCTTGTACGGCGACGGCGAAAACGTCCGCGACTGGCTGCACGTG
GACGACCACTGCCGTGGCATCGCCCTGGTCGCCGAACGCGGCAGGCCCGGCGAGATCTAC
CACATCGGAGGCGGGACGGAGCTGTCCAACCGTGAGCTCACCGCCCGGCTCCTGGACCTG
CTCGGCGTGGACTGGTCGATGGTCGAACCCGTGACCGACCGCAAGGGGCACGACCGGCGC
TACTCGCTGGACATCAGCAAGATCTCCGCCGAGCTCGGTTACGCGCCCCGCGTGCCCTTC
GAGGAGGGGCTGGCACAGACCGTTCAGTGGTACGTGGAGAACCGAACCCTGTGGGAGCCA
CTCACCGCCCGGCCCGAGCTGCCGGTCAGTGACGGCGCGTCCGGCGCAGAAACCGCTCGG
TCTCGTCCGCTGCCCGCAGGGCGCCGCCCGCCTCGACCGTGGCCCGCTGCATCTGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR005888 dTDP-glucose 4,6-dehydratase (Family) TIGR01181 dTDP_gluc_dehyt: dTDP-glucose 4,6-dehydratase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 no description
SignalP	No significant hit
TMHMM	No significant hit

Aver_00110 Aver_00130