Aver_00120 Aver_00120   Aver_00140 Aver_00140

Aver_00130 : CDS information

close this sectionLocation

Organism
StrainATCC 31267 (=NBRC 14893)
Entry nameAvermectin
Contig
Start / Stop / Direction74,993 / 74,094 / - [in whole cluster]
4,038 / 3,139 / - [in contig]
Locationcomplement(74094..74993) [in whole cluster]
complement(3139..4038) [in contig]
TypeCDS
Length900 bp (299 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative glucose-1-phosphate thymidylyltransferase
putative dTDP-glucose synthase
Product (GenBank)glucose-1-phosphate thymidyltransferase
Gene
Gene (GenBank)aveBIII
EC number2.7.7.24
Keyword
  • L-oleandrose
Note
Note (GenBank)
  • function in oleandrose biosynthesis
Reference
ACC
PmId
[10449723] Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis. (Proc Natl Acad Sci U S A. , 1999)
[11451669] Insights about the biosynthesis of the avermectin deoxysugar L-oleandrose through heterologous expression of Streptomyces avermitilis deoxysugar genes in Streptomyces lividans. (Chem Biol. , 2001)
comment
[PMID:10449723](1999)
avermectin生合成gene clusterの報告。

AveBIII: alpha-D-Glucose-1-phosphate thymidylyltransferase
この論文で実験確認しているわけではない。

---
[PMID: 11451669](2001)
S.lividansでS.avermitilis avrBCDEFGHIを発現させて外因性aglyconeと反応させて産物解析。
thymidylyl transferase (avrD/aveBIII)と、続くdehydratase (avrC/aveBII)の反応は、細菌における6-deoxyhexoses形成で共通のfirst 2stepsである。
ということで特に実験的に確かめていはいない。
Related Reference
ACC
Q9AGY4
PmId
[12147463] Homologs of the Rml enzymes from Salmonella enterica are responsible for dTDP-beta-L-rhamnose biosynthesis in the gram-positive thermophile Aneurinibacillus thermoaerophilus DSM 10155. (Appl Environ Microbiol. , 2002)
comment
65th(Q9AGY4) 58%, 8e-98
Aneurinibacillus thermoaerophilus_rmlA
Glucose-1-phosphate thymidylyltransferase(EC 2.7.7.24)

活性測定あり

close this sectionSequence

selected fasta
>putative glucose-1-phosphate thymidylyltransferase [glucose-1-phosphate thymidyltransferase]
MKGIVLAGGTGSRLYPLTRALSKQLLPVYDKPMIYYPLSVLMLGGIKDILVISSPDHLEQ
FRRLLGDGSRLGLNIDYAAQQRPGGIAEAFLIGADFIGQDQVSLVLGDNIFHGMGFSHLL
RSHTRDVDGCVLFGYAVTDPERYGVGEVDASGKLLSVEEKPTAPRSNLAITGLYLYDNDV
IEVARGIRSSARGELEITDVNRAYLAEGRARLVDLGRGFTWLDAGTHDSLMHAGQYVQVL
EKRQGVRIACLEEIAFRMGLIDADDCYLRGVELAGSGYGEYLMSIAAEAAVRSPGCAYS
selected fasta
>putative glucose-1-phosphate thymidylyltransferase [glucose-1-phosphate thymidyltransferase]
ATGAAGGGAATCGTGCTCGCGGGGGGAACCGGCTCTCGGCTTTACCCGTTGACCCGTGCC
CTTTCCAAGCAGCTGCTCCCTGTCTACGACAAACCCATGATCTACTACCCGCTCTCCGTG
TTGATGCTCGGCGGCATAAAAGACATCCTGGTGATTTCCTCACCCGATCATCTCGAACAA
TTCCGGAGACTGCTCGGAGACGGAAGCCGCCTCGGGCTGAACATCGACTACGCCGCGCAG
CAGCGACCGGGAGGAATCGCCGAGGCATTCCTCATCGGCGCCGATTTCATCGGTCAGGAT
CAGGTGTCCCTTGTCCTTGGGGACAACATCTTCCATGGCATGGGCTTTTCGCACCTGCTG
CGCAGCCACACCCGCGATGTCGACGGCTGTGTGTTGTTCGGATACGCGGTGACGGATCCC
GAACGGTACGGAGTCGGAGAGGTCGACGCCTCCGGAAAGCTGCTGTCCGTCGAGGAGAAA
CCCACCGCCCCCCGGTCCAACCTGGCGATCACAGGCCTGTACCTGTACGACAACGACGTG
ATCGAGGTGGCCCGGGGCATCCGCTCGTCGGCCCGCGGCGAGCTGGAGATCACCGACGTC
AACAGGGCCTACCTCGCCGAGGGGCGGGCCCGGCTGGTGGACCTGGGCCGGGGCTTCACC
TGGCTCGATGCGGGGACGCACGACTCGCTGATGCACGCCGGGCAGTATGTGCAGGTCCTG
GAGAAGCGCCAGGGAGTACGGATCGCCTGTCTCGAGGAGATCGCCTTCCGGATGGGTCTG
ATCGACGCGGACGACTGCTATCTGCGCGGGGTCGAACTGGCCGGCTCCGGCTACGGCGAG
TATCTGATGAGTATTGCGGCGGAAGCCGCGGTGAGGAGCCCGGGATGCGCCTACTCGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR005835 Nucleotidyl transferase (Domain)
[2-236] 1.9e-61 PF00483
PF00483   NTP_transferase
IPR005907 Glucose-1-phosphate thymidylyltransferase, short form (Family)
[2-286] 7.20000000000003e-127 TIGR01207
TIGR01207   RmlA
SignalP
[1-20] 0.252 Signal
Eukaryota   
TMHMM No significant hit
Aver_00120 Aver_00120   Aver_00140 Aver_00140
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