Aver_00140 : CDS information

Location

Organism	Streptomyces avermitilis
Strain	ATCC 31267 (=NBRC 14893)
Entry name	Avermectin
Contig	AB032523
Start / Stop / Direction	75,119 / 76,150 / + [in whole cluster] 4,164 / 5,195 / + [in contig]
Location	75119..76150 [in whole cluster] 4164..5195 [in contig]
Type	CDS
Length	1,032 bp (343 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative NDP-hexose C-4 ketoreductase
Product (GenBank)	dTDP-4-keto-6-deoxy-L-hexose 4-reductase
Gene
Gene (GenBank)	aveBIV
EC number
Keyword	L-oleandrose
Note
Note (GenBank)	function in oleandrose biosynthesis
Reference	ACC Q9S0P3 PmId [10449723] Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis. (Proc Natl Acad Sci U S A. , 1999) [11451669] Insights about the biosynthesis of the avermectin deoxysugar L-oleandrose through heterologous expression of Streptomyces avermitilis deoxysugar genes in Streptomyces lividans. (Chem Biol. , 2001) comment [PMID:10449723](1999) avermectin生合成gene clusterの報告。 AveBIV: dTDP-4-keto-6-deoxy-L-hexose 4-reductase この論文で実験確認しているわけではない。 --- [PMID: 11451669](2001) avrE/aveBIV: dTDP-4-ketohexulose reductase 4-ketoreductase_AvrEはaglyconeへ糖を付着する前の最終ステップを触媒すると考えられている。 S.lividansでS.avermitilis avrBCDEFGHIを発現させて外因性aglyconeと反応させて産物解析。どちらもketoreductaseをコードするとされるavrIとavrEを欠いたdelta-avrIEの発現ではderivativesも産生されるがavermectin A1aの産生も可能。よって代替経路がある模様。
Related Reference	ACC Q2PC38 NITE Rubra_00520 PmId [16614891] Functional characterization of ketoreductase (rubN6) and aminotransferase (rubN4) genes in the gene cluster of Streptomyces achromogenes var. rubradiris. (Biotechnol Lett. , 2006) comment 5th(Q2PC38) 53%, 9e-72 Streptomyces achromogenes subsp. rubradiris_rubN6 [Rubra_00520] NDP-hexose C-4 ketoreductase rubN6 and rubN4をHis-tagged fusion proteinsとしてE.coliで異種性に発現。それぞれのdTDP-glucose 4-ketoreductase and dTDP-glucose 3-aminotransferaseの機能性を、in vitro enzyme assayによって検証。

Sequence

>putative NDP-hexose C-4 ketoreductase [dTDP-4-keto-6-deoxy-L-hexose 4-reductase]
MGRFSVCPPRPTGILKSMLTTGMCDRPLVVVLGASGYIGSAVAAELARWPVLLRLVARRP
GVVPPGGAAETETRTADLTAASEVALAVTDADVVIHLVARLTQGAAWRAAESDPVAERVN
VGVMHDVVAALRSGRRAGPPPVVVFAGSVYQVGRPGRVDGSEPDEPVTAYARQKLDAERT
LKSATVEGVLRGISLRLPTVYGAGPGPQGNGVVQAMVLRALADEALTVWNGSVVERDLVH
VEDVAQAFVSCLAHADALAGRHWLLGSGRPVTVPHLFGAIAAGVSARTGRPAVPVTAVDP
PAMATAADFHGTVVDSSAFRAVTGWRPRLSLQEGLDHMVAAYV

>putative NDP-hexose C-4 ketoreductase [dTDP-4-keto-6-deoxy-L-hexose 4-reductase]
ATGGGGCGGTTTTCGGTGTGCCCGCCCCGGCCGACCGGAATACTGAAGAGCATGCTGACG
ACTGGGATGTGCGACCGACCGCTGGTCGTCGTACTCGGAGCCTCCGGCTATATCGGGTCG
GCCGTCGCGGCGGAACTCGCCCGGTGGCCGGTCCTGTTGCGGCTGGTGGCCCGGCGACCG
GGCGTCGTTCCGCCGGGCGGCGCCGCGGAGACCGAGACGCGTACGGCCGACCTGACGGCG
GCGAGCGAGGTCGCCCTCGCCGTGACGGACGCCGACGTGGTGATCCACCTGGTCGCGCGC
CTCACCCAGGGAGCGGCATGGCGGGCGGCGGAGAGCGATCCGGTGGCCGAGCGGGTGAAC
GTCGGGGTGATGCACGACGTCGTCGCGGCCCTGCGGTCCGGGCGCCGCGCCGGGCCGCCC
CCGGTGGTGGTGTTCGCCGGGTCGGTCTACCAGGTGGGCCGCCCGGGTCGGGTCGACGGC
AGTGAGCCGGACGAGCCCGTGACGGCCTATGCCCGTCAGAAACTCGACGCCGAACGGACG
TTGAAGTCCGCCACGGTCGAGGGTGTCCTGCGGGGGATCTCGCTGCGGCTGCCCACCGTC
TACGGCGCGGGGCCGGGCCCGCAGGGCAACGGCGTCGTGCAGGCGATGGTGCTCCGGGCG
CTCGCCGACGAGGCCCTCACCGTGTGGAACGGAAGCGTGGTGGAGCGTGACCTGGTGCAT
GTGGAGGATGTCGCGCAGGCCTTCGTGAGCTGCCTGGCGCACGCGGATGCGCTCGCCGGG
CGGCACTGGCTGCTCGGCAGCGGTCGTCCTGTGACCGTCCCGCACCTCTTCGGTGCCATC
GCCGCCGGCGTGTCCGCCCGCACCGGGCGCCCCGCGGTGCCCGTGACCGCGGTGGACCCT
CCGGCGATGGCGACGGCGGCGGACTTCCACGGGACCGTCGTCGACTCCTCGGCGTTCCGC
GCGGTCACCGGGTGGCGGCCGCGGCTGTCGCTTCAGGAGGGCCTGGACCACATGGTGGCG
GCTTACGTGTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001509 NAD-dependent epimerase/dehydratase (Domain) PF01370 Epimerase IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd
SignalP	Bacteria, Gram-negative Eukaryota
TMHMM	No significant hit

Aver_00130 Aver_00150