Aver_00140 Aver_00140   Aver_00160 Aver_00160

Aver_00150 : CDS information

close this sectionLocation

Organism
StrainATCC 31267 (=NBRC 14893)
Entry nameAvermectin
Contig
Start / Stop / Direction76,881 / 76,204 / - [in whole cluster]
5,926 / 5,249 / - [in contig]
Locationcomplement(76204..76881) [in whole cluster]
complement(5249..5926) [in contig]
TypeCDS
Length678 bp (225 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductNDP-hexose 5-epimerase
Product (GenBank)dTDP-4-keto-6-deoxyhexose 3,5-epimerase
Gene
Gene (GenBank)aveBV
EC number5.1.3.-
Keyword
  • L-oleandrose
Note
Note (GenBank)
  • function in oleandrose biosynthesis
Reference
ACC
PmId
[10449723] Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis. (Proc Natl Acad Sci U S A. , 1999)
[11451669] Insights about the biosynthesis of the avermectin deoxysugar L-oleandrose through heterologous expression of Streptomyces avermitilis deoxysugar genes in Streptomyces lividans. (Chem Biol. , 2001)
comment
[PMID:10449723](1999)
avermectin生合成gene clusterの報告。

AveBV: dTDP-4-keto-6-deoxyhexose 3,5-epimerase
この論文で実験確認しているわけではない。

---
[PMID: 11451669](2001)
Table1.
avrF/aveBV: dTDP-4-keto-6-deoxyglucose 3-epimerase　
(本文を読むと5-epimeraseの間違いと思われる。)

S.lividansでS.avermitilis avrBCDEFGHIを発現させて外因性aglyconeと反応させて産物解析。
delta-avrFな発現株では、C-13にD-olivoseが1つbeta-glycoside結合したavermectin A1a3を産生する。
avermectin A1a3を産生できるのはdelta-avrFのみ。
C-3での立体化学は最終的に3-O-methyltransferaseによって決定されるので、AvrF proteinは5-epimeraseであって3,5-epimeraseではない。

close this sectionSequence

selected fasta
>NDP-hexose 5-epimerase [dTDP-4-keto-6-deoxyhexose 3,5-epimerase]
MTLGRPRRSSADRPAPPAGARATAAGVTVRRLVVEGAVEFTPTVFPDERGLFVTPYQEPV
LSEAVGHRFPTAQTCQSVSRRGVVRGVHFTATPPGQAKYVHCARGRALDFVVDLRTGSPT
FGQWDSVLLDQERFRSVYLPIGVGHAFVALEDDTAMVYLMSSGYVPQNEHALSPEDPDLA
LPLGHHLGRAPILSERDRRAPTLQQALRRGMLPEYRASRGLDEKL
selected fasta
>NDP-hexose 5-epimerase [dTDP-4-keto-6-deoxyhexose 3,5-epimerase]
ATGACCCTTGGCCGGCCTCGCCGGAGCTCCGCCGACCGGCCCGCCCCGCCCGCGGGCGCC
CGGGCCACCGCCGCCGGTGTCACGGTCCGGAGGCTCGTCGTCGAGGGCGCCGTCGAATTC
ACCCCGACGGTCTTCCCCGACGAGCGCGGGCTCTTCGTCACCCCCTACCAGGAACCGGTC
CTCAGCGAGGCCGTCGGGCACCGGTTTCCCACCGCGCAGACCTGCCAGAGCGTCTCGCGC
CGCGGCGTCGTCCGCGGCGTGCACTTCACCGCGACACCACCCGGCCAGGCCAAGTACGTC
CACTGCGCCCGGGGACGGGCCCTGGACTTCGTCGTCGACCTGCGCACCGGCTCACCGACT
TTCGGGCAGTGGGACTCGGTCCTCCTCGACCAGGAGCGCTTCCGTTCCGTCTACCTGCCG
ATCGGGGTCGGGCACGCGTTCGTGGCGCTGGAAGACGACACGGCGATGGTGTACCTGATG
TCGAGCGGCTACGTTCCGCAGAACGAACACGCCCTCTCGCCCGAGGATCCGGACCTCGCC
CTGCCCCTCGGCCACCACCTCGGGCGGGCACCGATCCTCTCCGAGCGCGACCGGCGCGCG
CCGACTCTCCAGCAGGCCCTGCGACGCGGCATGTTGCCGGAGTACCGCGCGAGCCGCGGC
CTCGACGAGAAGCTGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000888 dTDP-4-dehydrorhamnose 3,5-epimerase-related (Family)
[30-205] 2.89999999999999e-56 PF00908
PF00908   dTDP_sugar_isom
[32-210] PD001462
PD001462   dTDP_sugar_isom
IPR011051 RmlC-like cupin domain (Domain)
[27-210] 1.40000506907309e-64 SSF51182
SSF51182   RmlC_like_cupin
IPR014710 RmlC-like jelly roll fold (Domain)
[28-216] 3.89999999999997e-59 G3DSA:2.60.120.10
G3DSA:2.60.120.10   RmlC-like_jellyroll
SignalP
[1-11] 0.499 Signal
Bacteria, Gram-negative   
[1-22] 0.424 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Aver_00140 Aver_00140   Aver_00160 Aver_00160
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