Aver_00160 Aver_00160   Aver_00180 Aver_00180

Aver_00170 : CDS information

close this sectionLocation

Organism
StrainATCC 31267 (=NBRC 14893)
Entry nameAvermectin
Contig
Start / Stop / Direction79,122 / 78,349 / - [in whole cluster]
8,167 / 7,394 / - [in contig]
Locationcomplement(78349..79122) [in whole cluster]
complement(7394..8167) [in contig]
TypeCDS
Length774 bp (257 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
ProductNDP-hexose C-3 O-methyltransferase
Product (GenBank)dTDP-6-deoxy-L-hexose 3-O-methyltransferase
Gene
Gene (GenBank)aveBVII
EC number2.1.1.-
Keyword
  • L-oleandrose
Note
Note (GenBank)
  • function in oleandrose biosynthesis
Reference
ACC
PmId
[10449723] Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis. (Proc Natl Acad Sci U S A. , 1999)
[11451669] Insights about the biosynthesis of the avermectin deoxysugar L-oleandrose through heterologous expression of Streptomyces avermitilis deoxysugar genes in Streptomyces lividans. (Chem Biol. , 2001)
comment
[PMID:10449723](1999)
avermectin生合成gene clusterの報告。

AveBVII: dTDP-6-deoxy-L-hexose 3-O-methyltransferase
この論文で実験確認しているわけではない。

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[PMID: 11451669](2001)
avrH/aveBVII: dTDP-6-deoxy-L-hexose 3-O-methyltransferase

S.lividansでS.avermitilis avrBCDEFGHIを発現させて外因性aglyconeと反応させて産物解析。
delta-avrHな発現株では、C-3でmethyl化されていないsugar(L-olivose, L-digitoxose)を持ったavermectin derivativesを産生する。

AvrH methyltransferaseはC-3でequatorialなhydroxyl基をmethylateする。axial C-3 hydroxyl基はできない。C-3の立体配置はAvrH methyltransferaseによって最終的に決定される。

close this sectionSequence

selected fasta
>NDP-hexose C-3 O-methyltransferase [dTDP-6-deoxy-L-hexose 3-O-methyltransferase]
MTEREFTDPRIVPHESEQERAAREQLTKLLVDSPIPPKYLIDNLSVYMRRNQLADLLSMD
ALYRMLPEVPGVIMEFGVLHGRHLATLTALRSIYEPYNSLRRVIGFDTFTGFPDIDEADE
VSTSAVPGRFAVPDGEVEHLRQVLAAHEANEPYGHTQRSFVVQGDVRETVPQYLAEHPHT
VIALAYFDLDLYRPTRELLDVITPHLTRGSILAFDELTHPKWPGETRALSEAFGLDHAPL
RQLPGREPPVIYMTWGA
selected fasta
>NDP-hexose C-3 O-methyltransferase [dTDP-6-deoxy-L-hexose 3-O-methyltransferase]
ATGACCGAGCGGGAGTTCACCGATCCCCGGATCGTGCCGCACGAGTCCGAGCAGGAACGC
GCTGCCCGCGAGCAGCTGACCAAGCTCCTCGTCGACAGCCCGATCCCGCCGAAGTACCTG
ATCGACAACCTGTCCGTCTACATGCGGCGCAACCAGCTCGCCGACCTGCTCTCCATGGAC
GCGCTGTACCGCATGCTGCCGGAAGTCCCCGGCGTCATCATGGAGTTCGGCGTTCTGCAC
GGACGGCACCTCGCCACGCTGACCGCGCTGCGCAGCATCTATGAGCCGTACAACTCGCTG
CGCCGCGTCATCGGCTTCGACACGTTCACCGGCTTCCCCGACATCGACGAGGCGGACGAG
GTCAGCACCAGCGCGGTCCCTGGGCGGTTCGCCGTGCCGGACGGCGAAGTGGAGCACCTG
CGGCAGGTGCTCGCGGCGCACGAGGCGAACGAACCCTACGGCCACACCCAGCGTTCCTTC
GTGGTCCAGGGCGACGTACGCGAAACGGTGCCCCAATACCTGGCGGAACACCCGCACACC
GTCATCGCGCTGGCCTACTTCGACCTGGACCTCTACCGCCCCACGCGCGAACTGCTGGAC
GTCATCACACCGCACCTGACCAGGGGCAGCATCCTCGCCTTCGACGAACTCACCCACCCC
AAATGGCCGGGGGAGACCAGGGCGCTGAGCGAGGCGTTCGGCCTCGACCACGCCCCACTG
CGCCAACTGCCCGGCCGCGAGCCACCGGTGATCTACATGACGTGGGGTGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR008884 Macrocin-O-methyltransferase (Family)
[68-218] 6.3e-05 PF05711
PF05711   TylF
SignalP No significant hit
TMHMM No significant hit
Aver_00160 Aver_00160   Aver_00180 Aver_00180
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