Adria_00110 : CDS information

Location

Organism	Streptomyces peucetius
Strain	ATCC 29050 (=NBRC 100596)
Entry name	Doxorubicin
Contig	L37338
Start / Stop / Direction	3,767 / 4,789 / + [in whole cluster] 1,085 / 2,107 / + [in contig]
Location	3767..4789 [in whole cluster] 1085..2107 [in contig]
Type	CDS
Length	1,023 bp (340 aa)

Annotation

Category	4.1 transcriptional regulator
Product	transcriptional activator/repressor
Product (GenBank)	putative repressor
Gene
Gene (GenBank)	dnrO
EC number
Keyword
Note
Note (GenBank)	putative
Reference	ACC Q54822 PmId [7868594] Regulation of daunorubicin production in Streptomyces peucetius by the dnrR2 locus. (J Bacteriol. , 1995) [10846224] The dnrO gene encodes a DNA-binding protein that regulates daunorubicin production in Streptomyces peucetius by controlling expression of the dnrN pseudo response regulator gene. (Microbiology. , 2000) [20122958] The activator/repressor protein DnrO of Streptomyces peucetius binds to DNA without changing its topology. (Int J Biol Macromol. , 2010) [20529134] Modulation of dnrN expression by intracellular levels of DnrO and daunorubicin in Streptomyces peucetius. (FEMS Microbiol Lett. , 2010) [20158521] Daunorubicin efflux in Streptomyces peucetius modulates biosynthesis by feedback regulation. (FEMS Microbiol Lett. , 2010) comment [PMID: 7868594](1995) dnrR2 locusの解析。 dnrOについては配列解析のみ。repressorに似ている。 --- 続報いくつかあり。 [PMID: 10846224](2000) dnrO::aphII mutant strainはdaunorubicin (DNR)非産生。このmutant strainにdnrN or dnrI を導入すると生合成可能。 dnrI 発現はDnrNが必要なことがわかっているので、dnrN発現にDnrOが必要ということが示される。 DnrO → dnrN = DnrN → dnrI = DnrI →DNR生合成genes DnrOをE.coliで過剰発現し、抽出液でmobility shift DNA-binding assays。 DnrOが特異的にoverlapping dnrN/dnrO(p1) promoter regionへ結合するので、DnrOはdnrNとdnrOの両方の発現を調節するかもしれない。 --- その後、別グループでも報告あり。 [PMID: 20122958][PMID: 20529134][PMID: 20158521](2010) dnrO, dnrN, dnrI, DNRが関与する細胞内DNR濃度維持システムが提唱されている。 DnrOはDnrNと競合してfeedback的にDNRの細胞内濃度を調節している。

Sequence

>transcriptional activator/repressor [putative repressor]
MTETPSRMLRLLSLLQNSRDWPGRKLAERLGVSPRTLRRDVERLRDLGYPVQAIPGVGGG
YRLEAGASLPPLLLDDDEAVAIAVGLRTAAGGSVADIEDTSIRALAKLEQVLPKRLRSRV
NALQMYTVPMFTVTPTVSPEHLASVAQACRDQQRLRFTYRKRDGETSSRLVEPYRLVHVN
RKWYVLSWDMGRQDWRTFRVDRMESLLLTGVRFQQRELPAEDAAAYVAQSITSSLAGYRA
LVTLHAPVEVVAERIRPRDGILTAVDHRTCRLRSAADSLEWLALTIGMFGVEFEVHEPPE
LIHYIQELSERLARAASPGADRSVVAPPTRDAPVSGPAAG

>transcriptional activator/repressor [putative repressor]
ATGACCGAGACGCCATCCCGCATGCTCCGTCTGCTGTCCCTCCTCCAGAACAGCCGGGAC
TGGCCAGGGCGCAAGCTGGCGGAGCGGCTCGGGGTCAGCCCGCGGACGCTGCGCCGAGAC
GTGGAACGGTTGCGGGATCTCGGCTACCCGGTTCAGGCCATACCGGGCGTCGGCGGAGGC
TACCGGCTGGAGGCGGGCGCCAGTCTGCCGCCGCTGCTCCTCGACGACGACGAGGCGGTC
GCCATCGCGGTCGGATTACGGACGGCCGCAGGGGGAAGCGTGGCGGACATCGAGGACACC
TCGATCCGCGCGCTGGCCAAGCTGGAGCAGGTGCTGCCCAAGCGGCTGCGCAGCCGGGTC
AACGCCCTTCAGATGTACACCGTCCCCATGTTCACGGTGACCCCGACCGTGAGCCCCGAA
CACCTCGCGTCAGTGGCACAGGCGTGCCGCGACCAGCAGCGGCTGAGATTCACCTACCGC
AAGCGCGACGGGGAGACGAGCAGCCGTCTGGTGGAGCCGTACCGTCTGGTCCATGTGAAC
CGTAAGTGGTACGTGCTGTCCTGGGACATGGGACGGCAGGACTGGCGCACCTTCCGCGTG
GACCGCATGGAATCGCTGCTGTTGACGGGGGTGCGTTTCCAGCAGCGCGAGTTGCCTGCC
GAGGACGCGGCGGCCTATGTGGCGCAGTCGATCACGTCCTCGCTGGCCGGCTACCGGGCG
CTGGTCACCCTGCACGCGCCGGTCGAGGTGGTGGCGGAGCGGATCCGCCCGCGCGACGGA
ATCCTCACAGCGGTCGACCACCGGACATGCCGGCTGCGTTCGGCCGCCGACTCGCTGGAG
TGGCTGGCGCTCACCATCGGGATGTTCGGCGTCGAGTTCGAGGTGCACGAGCCGCCTGAA
CTGATTCATTACATACAGGAGTTGTCGGAGCGGCTGGCCCGAGCCGCGTCGCCGGGCGCG
GACCGCTCCGTGGTGGCGCCGCCGACGCGGGACGCTCCGGTCAGCGGGCCAGCAGCCGGG
TGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001034 HTH DeoR N-terminal domain (Domain) PS51000 HTH_DEOR_2 IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain) G3DSA:1.10.10.10 Wing_hlx_DNA_bd IPR013196 Helix-turn-helix, type 11 (Domain) PF08279 HTH_11 IPR018356 Transcription regulator, HTH DeoR-type, conserved site (Conserved_site) PS00894 HTH_DEOR_1
SignalP	Eukaryota
TMHMM	No significant hit

Adria_00100 Adria_00115