Aran_00080 Aran_00080   Aran_00100 Aran_00100

Aran_00090 : CDS information

close this sectionLocation

Organism
StrainTü303
Entry nameAranciamycin
Contig
Start / Stop / Direction14,120 / 13,854 / - [in whole cluster]
14,120 / 13,854 / - [in contig]
Locationcomplement(13854..14120) [in whole cluster]
complement(13854..14120) [in contig]
TypeCDS
Length267 bp (88 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase acyl carrier protein subunit
Product (GenBank)acyl carrier protein
Gene
Gene (GenBank)
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
  • ACP; orf9
Reference
ACC
PmId
[17357167] Cloning and heterologous expression of the aranciamycin biosynthetic gene cluster revealed a new flexible glycosyltransferase. (Chembiochem. , 2007)
comment
aranciamycin生合成gene cluster(ara-cluster)のクローニングとシークエンス報告。

ORF9(88aa): acyl-carrier protein

ORFs 11, 10, 9は典型的なtype II PKS編成で見つかり、おそらくketo-synthase(KS) alpha, KS beta, ACPをコードする。
Related Reference
ACC
Q54219
NITE
Griseu_00030
PmId
[8169211] Cloning, sequencing, and analysis of the griseusin polyketide synthase gene cluster from Streptomyces griseus. (J Bacteriol. , 1994)
[9108302] Post-translational modification of heterologously expressed Streptomyces type II polyketide synthase acyl carrier proteins. (FEBS Lett. , 1997)
[9738947] Acylation of Streptomyces type II polyketide synthase acyl carrier proteins. (FEBS Lett. , 1998)
comment
Blast 6th, id55%, 6e-16
Streptomyces griseus_ORF3
Acyl carrier protein

--
[PMID: 8169211](1994)
griseusin polyketide synthase gene clusterの報告。

このORF3を含んだ領域の置換で、griseusins A and B産生能力を損失。
ORF3単独の機能は、配列解析から述べられている。

--
[PMID: 9108302](1997)
E.coliにおけるactinorhodin (act), oxytetracycline (otc) and griseusin (gris) apo-ACPsのholo型形成に関する調査。

--
[PMID: 9738947](1998)
上記論文の続報。
holo-ACPsによるacylationの調査。

close this sectionPKS/NRPS Module

ACP5..78

close this sectionSequence

selected fasta
>polyketide synthase acyl carrier protein subunit [acyl carrier protein]
MAEMTLEQLTGLLRECAGEEEGVDLDGDVLDRAFTDLGYDSLAVLQVIGRVEQDYGLTLP
DEVVAEATTPRQFLARVNDGLSEVPRPA
selected fasta
>polyketide synthase acyl carrier protein subunit [acyl carrier protein]
ATGGCGGAGATGACCCTTGAGCAACTCACCGGCCTGCTGCGCGAGTGCGCGGGCGAGGAG
GAAGGGGTGGACCTGGACGGCGACGTCCTGGACCGCGCCTTCACCGACCTCGGCTACGAC
TCCCTGGCGGTGCTGCAGGTGATCGGCCGTGTCGAGCAGGACTACGGCCTCACGCTGCCG
GACGAGGTGGTGGCGGAGGCGACGACGCCACGCCAGTTCCTGGCGCGCGTGAACGACGGT
CTGAGCGAGGTGCCTCGGCCGGCGTGA
ACP5..78
ACP13..234

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR006162 Phosphopantetheine attachment site (PTM)
[36-51] PS00012
PS00012   PHOSPHOPANTETHEINE
IPR009081 Acyl carrier protein-like (Domain)
[5-78] PS50075
PS50075   ACP_DOMAIN
[1-81] 2.800005042611e-17 SSF47336
SSF47336   ACP_like
[5-81] 5.59999999999999e-16 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[13-77] 8.60000000000001e-11 PF00550
PF00550   PP-binding
SignalP
[1-18] 0.094 Signal
Eukaryota   
TMHMM No significant hit
Aran_00080 Aran_00080   Aran_00100 Aran_00100
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