Aran_00100 : CDS information

Location

Organism	Streptomyces echinatus
Strain	Tü303
Entry name	Aranciamycin
Contig	DQ915964
Start / Stop / Direction	15,370 / 14,144 / - [in whole cluster] 15,370 / 14,144 / - [in contig]
Location	complement(14144..15370) [in whole cluster] complement(14144..15370) [in contig]
Type	CDS
Length	1,227 bp (408 aa)

Annotation

Category	1.1 PKS
Product	polyketide synthase chain length factor subunit
Product (GenBank)	chain length factor
Gene
Gene (GenBank)
EC number
Keyword	type II PKS
Note
Note (GenBank)	CLF; orf10
Reference	ACC A1C178 PmId [17357167] Cloning and heterologous expression of the aranciamycin biosynthetic gene cluster revealed a new flexible glycosyltransferase. (Chembiochem. , 2007) comment aranciamycin生合成gene cluster(ara-cluster)のクローニングとシークエンス報告。 ORF10(408aa): chain length-factor ORFs 11, 10, 9は典型的なtype II PKS編成で見つかり、おそらくketo-synthase(KS) alpha, KS beta, ACPをコードする。
Related Reference	ACC Q56164 NITE Jado_00090 PmId [7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994) [9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997) comment Blast 16th, id65%, 1e-137 Streptomyces venezuelae_jadB Chain length determinant --- [PMID: 7881555](1994) ORF2(=JadBに相当)は配列解析からtype II PKSのCLFであると提唱されている。 ORF2-ORF4領域を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。 -- [PMID: 9224566](1997) minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。 jadBもKS-betaとして使用されている。

PKS/NRPS Module

CLF	8..363

Sequence

>polyketide synthase chain length factor subunit [chain length factor]
MSSTASPVFTGIGVTAPNGLGTEAWWRATLAGEHGIRPVSRFDATGYPATLAGEVPDYEP
ERRIPSRLLPQTDHMTRLALTAAEEALSDAKVDPDGLPEYTAGVVTAASAGGFEFGQREL
QALWSKGGQFVSAYQSFAWFYAVNTGQISIRHGLRGPSGVLVTEQAGGLDVVAQARRQLR
KGSALMVTGGVDGSVCPWGWTGHLAGGRMSTVAEPERAYLPFARDARGHVAGEGGAILIL
EDAAAARARGARTYGALAGYAATFDPAPGTGAEPGLRRAVELALDDAGLAAGDIDVVFAD
GAGIRDLDRQEADALARVFGPRGVPVTVPKTMTGRLLAGGAALDLAAALLSVHEGLIPPT
AHVTSPADDCPVDLVTAVRSGPVRHALVLARGHGGFNAAAVVTAVSDS

>polyketide synthase chain length factor subunit [chain length factor]
ATGAGCAGCACGGCATCCCCGGTCTTCACCGGCATCGGCGTCACGGCCCCCAACGGGCTG
GGCACCGAGGCCTGGTGGCGGGCCACCCTGGCGGGCGAGCACGGCATCCGTCCGGTCTCC
CGCTTCGACGCCACCGGCTATCCGGCCACCCTCGCCGGCGAGGTGCCCGACTACGAGCCG
GAGCGGCGCATCCCCAGCCGGCTGCTGCCGCAGACCGATCACATGACCCGGCTCGCGCTC
ACCGCCGCCGAGGAGGCGCTCTCCGACGCCAAGGTGGATCCGGACGGCCTGCCGGAGTAC
ACCGCCGGCGTGGTCACCGCCGCCTCGGCCGGTGGGTTCGAGTTCGGCCAGCGCGAGTTG
CAGGCCCTGTGGAGCAAGGGCGGACAGTTCGTCAGCGCGTACCAGTCCTTCGCCTGGTTC
TACGCCGTCAACACCGGCCAGATCTCCATCAGGCACGGGCTGCGCGGGCCGAGCGGCGTC
CTGGTCACCGAGCAGGCCGGCGGACTGGACGTGGTGGCCCAGGCCCGCCGTCAGCTGCGC
AAGGGCAGCGCGCTGATGGTGACCGGAGGCGTCGACGGGTCCGTCTGCCCCTGGGGCTGG
ACCGGTCATCTGGCAGGCGGACGCATGAGCACCGTCGCCGAACCGGAGCGCGCCTACCTG
CCGTTCGCCCGTGACGCCCGCGGCCATGTGGCCGGCGAGGGGGGCGCGATCCTGATCCTG
GAGGACGCCGCCGCGGCCCGGGCGCGCGGTGCCCGGACGTACGGCGCACTCGCCGGCTAC
GCCGCCACGTTCGACCCGGCGCCCGGCACCGGCGCCGAGCCCGGACTGCGCCGGGCGGTG
GAACTGGCTCTGGACGACGCCGGACTCGCCGCCGGCGACATCGACGTGGTCTTCGCCGAC
GGCGCCGGGATCCGCGACCTCGACCGGCAGGAGGCCGACGCCCTGGCGCGGGTCTTCGGT
CCCCGGGGCGTGCCGGTGACCGTCCCCAAGACGATGACCGGGCGGCTGCTGGCCGGTGGC
GCGGCCCTGGACCTGGCGGCGGCGCTGCTGTCGGTCCACGAGGGGCTGATCCCGCCGACC
GCCCATGTGACCTCCCCCGCCGACGACTGCCCCGTCGACCTGGTCACCGCCGTACGCAGC
GGCCCGGTACGCCACGCGCTGGTGCTCGCGCGCGGCCACGGCGGGTTCAACGCGGCCGCG
GTGGTGACCGCCGTGTCCGACAGCTGA

CLF	8..363

CLF	22..1089

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR014030 Beta-ketoacyl synthase, N-terminal (Domain) PF00109 ketoacyl-synt IPR014031 Beta-ketoacyl synthase, C-terminal (Domain) PF02801 Ketoacyl-synt_C IPR016038 Thiolase-like, subgroup (Domain) G3DSA:3.40.47.10 Thiolase-like_subgr IPR016039 Thiolase-like (Domain) SSF53901 Thiolase-like
SignalP	No significant hit
TMHMM	No significant hit

Aran_00090 Aran_00110