Aran_00230 Aran_00230   last last

Aran_00240 : CDS information

close this sectionLocation

Organism
StrainTü303
Entry nameAranciamycin
Contig
Start / Stop / Direction34,559 / 35,758 / + [in whole cluster]
34,559 / 35,758 / + [in contig]
Location34559..35758 [in whole cluster]
34559..35758 [in contig]
TypeCDS
Length1,200 bp (399 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.2 modification methylation
Productputative O-methyltransferase
Product (GenBank)methyltransferase
Gene
Gene (GenBank)
EC number2.1.1.-
Keyword
Note
  • The N-terminal position was modified from original INSDC entry.
Note (GenBank)
  • orf24
Reference
ACC
PmId
[17357167] Cloning and heterologous expression of the aranciamycin biosynthetic gene cluster revealed a new flexible glycosyltransferase. (Chembiochem. , 2007)
comment
aranciamycin生合成gene cluster(ara-cluster)のクローニングとシークエンス報告。

ORF24(351aa): MT

ORF24はara clusterに含まれる2つのmethyltransferasesのうちのひとつで、L-rhamnoseのC'-2へのmethyl基転移を触媒すると想定されている（配列解析）。
Related Reference
ACC
O54261
NITE
Nogl_00410
PmId
[22120896] Identification of late-stage glycosylation steps in the biosynthetic pathway of the anthracycline nogalamycin. (Chembiochem. , 2012)
comment
Blast 3rd, id55%, 4e-98
Streptomyces nogalater_snogY
SnogY
[DoBISCUIT]demethoxynogalose C-2' O-methyltransferase_snogY

aglycone生合成とdeoxysugar形成に必要な29ORFsを含むpSnogaoriを異種ホストS. albusに導入。
・3',4'-demethoxynogalose-1-hydroxynogalamycinone
・nogalamineがC-C結合ありrhodosamineで置換されたnogalamycin R
・nogalamineがC-C結合なし2-deoxyfucoseで置換されたnogalamycin F
がS. albus/pSnogaoriで産生された。

sno clusterにあるO-methyltransferase候補のうち、pSnogaoriにはsnogYが含まれるが、snogM and snogLは含まれない。
S. albus/pSnogaoriの産物はどれもnogalose部分のC2'にmethoxy基を含むので、SnogYがC2'でのO-methylationを担う。

提唱経路schemeでは、aglyconeに付着されてからメチル化を受けるとされている。
ACC
Q9AJU1
NITE
Ello_00190
PmId
[11376004] Deoxysugar methylation during biosynthesis of the antitumor polyketide elloramycin by Streptomyces olivaceus. Characterization of three methyltransferase genes. (J Biol Chem. , 2001)
comment
Blast 17th, id46%, 2e-68
Streptomyces olivaceus_elmMII
O-methyltransferase II(Rhamnose C3'-methyltransferase)

elmMI, elmMII, and elmMIIIをそれぞれ発現した株でのmethylation活性を確認。
sugar moiety rhamnoseはaglyconへ付着されてから3か所でメチル化される。
ACC
Q83WF2
PmId
[19415708] Functional analysis of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin macrolide antibiotics. (Chembiochem. , 2009)
comment
Blast 9th, id44%, 6e-81
Micromonospora griseorubida_mycE
Methyltransferase

mycinamicin生合成における2つのO-methyltransferase、MycE と MycFの機能を解析している。
mycE, mycFそれぞれの遺伝子をHis-tagを付けてE. coliで発現させて精製し、各々の機能を調べている。

各酵素はS-adenosyl-L-methionine (SAM)-dependent deoxysugar methyltransferaseであることが確認された。MycEの最適反応には、MycFよりも明白にMg(2+)が必要。

MycE → aglycone付着6-deoxyalloseのC2''-OHをmethylation = javose
MycF → aglycone付着javoseのC3''-OHをmethylation = mycinose

close this sectionSequence

selected fasta
>putative O-methyltransferase [methyltransferase]
MTPDSLVERLVLCGTAPDSETALLAKEAGLQRLTGALVDEVLFRCEQPVNTTPVDIALEV
THEQELRRTVLRMAAGEPIRVLDADDPTAVVRRHFRFSLTDLVRRLYGPLQKPHSRDFTD
VFLPERPADLTELPQILQATHQAGGTLLSGCTSDAPGLGALSVAYGSDKWASFHWYTQHY
EHHFARYRDRPVRILEIGIGGYAGDLGGPSLKMWKRYFHRGLVFGLDLFDKSALNQPRLT
ALTGDQGDAAGLRELAERYGPFDIVIDDGSHENEHVRISFDALFPYVRPGGLYVIEDLQT
AYWPRFGGTAGAETAPDTSVGLLKRLLDDLHHAEYDPKPDTPRTATQEQVIGVHTYHNIA
FVEKGVNGESSIPAWMDDEAWKALGAITPEEGRELGASR
selected fasta
>putative O-methyltransferase [methyltransferase]
ATGACTCCGGACAGCCTTGTCGAGCGACTCGTCCTGTGCGGAACAGCGCCCGACTCCGAG
ACCGCCCTCCTCGCGAAGGAGGCGGGGCTCCAGCGCCTGACCGGCGCCCTCGTGGACGAG
GTGCTGTTCCGCTGCGAGCAGCCCGTGAACACCACCCCCGTCGACATCGCGCTGGAGGTG
ACCCACGAGCAGGAACTGCGCCGCACGGTGCTGCGCATGGCGGCCGGCGAACCGATCCGG
GTCCTGGACGCCGACGACCCCACAGCCGTGGTCCGCCGTCACTTCCGGTTCTCCCTCACC
GACCTGGTCCGCCGCCTCTACGGCCCCCTCCAGAAGCCCCACAGCCGCGACTTCACCGAC
GTCTTCCTGCCCGAGCGGCCGGCCGACCTGACGGAGCTGCCGCAGATCCTGCAGGCCACG
CACCAGGCGGGCGGCACCCTGCTGTCCGGCTGCACGAGCGACGCCCCCGGCCTGGGAGCC
CTGTCCGTGGCGTACGGCTCCGACAAATGGGCCAGCTTCCACTGGTACACCCAGCACTAC
GAGCACCACTTCGCCCGCTACCGGGACCGGCCGGTGCGGATCCTGGAGATCGGCATCGGC
GGCTACGCGGGCGACCTGGGCGGGCCGTCGCTGAAAATGTGGAAGCGGTACTTCCACCGC
GGGCTCGTCTTCGGCCTCGACCTGTTCGACAAGTCGGCGCTGAACCAGCCCCGGCTCACC
GCCCTCACCGGCGACCAGGGCGACGCCGCCGGGCTGCGTGAACTCGCCGAGCGGTACGGC
CCGTTCGACATCGTCATCGACGACGGAAGCCACGAGAACGAGCACGTGCGGATCTCCTTC
GACGCGCTCTTCCCGTACGTACGCCCCGGCGGCCTGTACGTCATCGAGGACCTCCAGACC
GCGTACTGGCCGCGGTTCGGCGGCACCGCCGGCGCCGAGACCGCGCCCGACACCTCGGTC
GGCCTGCTCAAGCGGCTCCTGGACGACCTGCACCACGCGGAGTACGACCCCAAGCCCGAC
ACACCCCGCACGGCCACCCAGGAACAGGTGATCGGCGTGCACACGTACCACAACATCGCC
TTCGTCGAGAAGGGCGTCAACGGCGAGAGCAGCATCCCGGCCTGGATGGACGACGAGGCG
TGGAAGGCGCTCGGCGCCATCACCCCGGAGGAGGGCCGGGAGCTGGGGGCGTCGCGATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0 		No significant hit
SignalP No significant hit
TMHMM No significant hit
Aran_00230 Aran_00230   last last
Page top