Aran_00220 Aran_00220   Aran_00240 Aran_00240

Aran_00230 : CDS information

close this sectionLocation

Organism
StrainTü303
Entry nameAranciamycin
Contig
Start / Stop / Direction33,305 / 34,192 / + [in whole cluster]
33,305 / 34,192 / + [in contig]
Location33305..34192 [in whole cluster]
33305..34192 [in contig]
TypeCDS
Length888 bp (295 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative C-7 ketoreductase
Product (GenBank)oxidoreductase
Gene
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • orf23
Reference
ACC
PmId
[17357167] Cloning and heterologous expression of the aranciamycin biosynthetic gene cluster revealed a new flexible glycosyltransferase. (Chembiochem. , 2007)
comment
aranciamycin生合成gene cluster(ara-cluster)のクローニングとシークエンス報告。

ORF23(295aa): oxidoreductase

詳細記載なし。
Related Reference
ACC
Q9RN66
NITE
Nogl_00130
PmId
[11683270] The entire nogalamycin biosynthetic gene cluster of Streptomyces nogalater: characterization of a 20-kb DNA region and generation of hybrid structures. (Mol Genet Genomics. , 2001)
comment
Blast 3rd, id60%, 3e-74
Streptomyces nogalater_snoaF
C-7 ketoreductase

S. nogalater genomic DNA fragmentの配列解析と、異種性発現による産物解析。
nogalamycin生合成に関する20ORFsが明らかになった。

snoaF: C7-ketoreductase

aklaviketoneを蓄積するS.galilaeus H036 mutantは、snoaFをpromoter下においたplasmid pSYE57によって異種性に相補される。
SnoaFによるaklaviketone→aklavinoneへの変換は、aglyconへsugar moietyが付着できるようにし、aklavinone glycosidesを蓄積するようになる。

nogalamycin生合成経路での割り当ては、
nogalaviketone → nogalamycinone へのC-7還元が提唱されている。
nogalaviketoneとaklaviketoneの違いはstarter(acetate or propionate)のみ。
ACC
Q53882
NITE
Dauno_00060
PmId
[8655529] Cloning, sequencing, and analysis of aklaviketone reductase from Streptomyces sp. strain C5. (J Bacteriol. , 1996)
comment
Blast 8th, id61%, 6e-69
Streptomyces sp. (strain C5)_dauE
Aklaviketone reductase

recombinant DauE を含んだStreptomyces lividans TK24 の抽出液は、
aklaviketone → aklavinone
maggiemycin → epsilon-rhodomycinone(RHO)
7-oxodaunomycinone → daunomycinone
へのNADPH-specificな変換を触媒した。
これらの変換はすべてposition 7でのketo基の還元。

close this sectionSequence

selected fasta
>putative C-7 ketoreductase [oxidoreductase]
MTDYSHDPDSPNPSAPTAAPSAHSPTAPSAHSPTAPSAQAPTTPAASPPDRAVVVTGGGT
GIGRAVAHRFAEEGARVLVVGRTPGTLEETARHHPGIRALPADITAPDGPDTVVSAALER
FGRIDVLVNNAALGIAGTLDTLDPADLDAQLATNLRAPMLLTRRALPALEKSSGTVVNLS
TAGSLGRRAWPGMSVYGATKTALDFLTRTWAVELAPRGIRVVGLAPGVIDTGMATRNGAS
EEQYAAFLDGIRSRVPAGRVGRPEEIAWWVARLADPLAGYAHGTVLAVDGALSLQ
selected fasta
>putative C-7 ketoreductase [oxidoreductase]
GTGACCGACTACAGCCACGACCCCGACTCACCCAACCCCTCCGCACCCACCGCTGCCCCC
TCCGCCCACTCACCCACCGCCCCCTCCGCCCACTCACCCACCGCCCCCTCGGCGCAGGCA
CCCACCACCCCCGCCGCGTCCCCGCCCGACCGGGCCGTCGTCGTGACCGGTGGCGGGACC
GGCATCGGTCGAGCCGTCGCCCACCGCTTCGCCGAGGAAGGTGCCCGGGTCCTCGTCGTG
GGCCGGACCCCGGGCACGCTGGAGGAGACCGCGCGCCATCACCCCGGCATCCGCGCACTC
CCCGCCGACATCACCGCGCCCGACGGCCCGGACACCGTCGTCTCGGCCGCGCTGGAGCGC
TTCGGCCGGATCGACGTCCTGGTGAACAACGCCGCCCTCGGCATCGCGGGCACCCTGGAC
ACCCTGGACCCCGCCGACCTGGACGCCCAGCTCGCGACGAACCTCCGGGCCCCGATGCTG
CTGACCCGCCGCGCGCTCCCCGCGCTGGAGAAGAGCTCCGGCACGGTCGTCAACCTCAGC
ACGGCCGGCTCGCTCGGCCGCCGGGCCTGGCCCGGCATGTCGGTCTACGGCGCCACCAAG
ACCGCGCTGGACTTCCTGACCCGGACCTGGGCGGTGGAACTCGCCCCCCGCGGGATCCGG
GTGGTGGGACTGGCACCCGGTGTCATCGACACCGGCATGGCGACCCGCAACGGCGCGAGC
GAGGAGCAGTACGCGGCCTTCCTCGACGGCATCCGCTCCCGCGTCCCGGCCGGACGCGTC
GGCCGCCCCGAGGAGATCGCCTGGTGGGTGGCCCGGCTCGCCGACCCGCTCGCCGGCTAC
GCCCACGGCACCGTCCTCGCCGTCGACGGCGCCCTGTCCCTGCAGTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[52-214] 2.6e-28 PF00106
PF00106   adh_short
[122-133] 6.69999687625999e-09 PR00080 [174-182] 6.69999687625999e-09 PR00080 [196-215] 6.69999687625999e-09 PR00080
PR00080   SDRFAMILY
[17-294] 2.79999889404114e-16 PIRSF000126
PIRSF000126   11-beta-HSD1
IPR002347 Glucose/ribitol dehydrogenase (Family)
[52-69] 1.49999648196323e-36 PR00081 [122-133] 1.49999648196323e-36 PR00081 [168-184] 1.49999648196323e-36 PR00081 [196-215] 1.49999648196323e-36 PR00081 [217-234] 1.49999648196323e-36 PR00081 [256-276] 1.49999648196323e-36 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[50-294] 2.90000000000003e-76 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-40] 0.377 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Aran_00220 Aran_00220   Aran_00240 Aran_00240
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