Fred_00140 Fred_00140   Fred_00160 Fred_00160

Fred_00150 : CDS information

close this sectionLocation

Organism
StrainATCC 49344
Entry nameFredericamycin
Contig
Start / Stop / Direction13,560 / 13,111 / - [in whole cluster]
13,560 / 13,111 / - [in contig]
Locationcomplement(13111..13560) [in whole cluster]
complement(13111..13560) [in contig]
TypeCDS
Length450 bp (149 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.1 modification hydroxylation
Producthydroxylase
Product (GenBank)putative monooxygenase
GenefdmM
Gene (GenBank)
EC number
Keyword
  • pentangular C-14
Note
Note (GenBank)
  • FdmM
Reference
ACC
PmId
[16305230] Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from Streptomyces griseus. (J Am Chem Soc. , 2005)
[19620242] In vivo investigation of the roles of FdmM and FdmM1 in fredericamycin biosynthesis unveiling a new family of oxygenases. (J Biol Chem. , 2009)
comment
[PMID: 16305230](2005)
Fredericamycin(FDM) gene clusterの同定論文。

fdmM: oxygenase

FdmJ, L, M, M1, P, Qはantibiotic monooxygenase domainを持つ。
(interpro:26.0でIPR007138 Antibiotic biosynthesis monooxygenase domainが確認できるのはFdmJ, P, Qのみ）

fdmC-fdmT3のfragmentをS.albusへ導入し、FDM-A産生に十分であることを確認している。

---
[PMID: 19620242](2009)
delta-fdmM, delta-fdmM1 mutantを作成し産物解析。
FDM生合成においてFdmMはC-6、FdmM1はC-8での hydroxylationsを触媒する。
これらgenesのhomologはこれまで機能不明タンパクとされており、新しいoxygenase familyといえる。

C-6, C-8はpolyketideでのpositionに相当。

close this sectionSequence

selected fasta
>hydroxylase [putative monooxygenase]
MMLEEVLSGVVLAGTGTVTGVFVAVAVSVGPAMSAMGRAEYLRAHALLGKGYHPLMPILV
NIVTLSGFALAFLAGAPGNVLFPLASVLLIGVQAVSHLGNVPINRSLSGLAAAGSWVDPR
PQWRAWHRLRTVLAALALAAVTVAVLTAR
selected fasta
>hydroxylase [putative monooxygenase]
GTGATGCTCGAGGAAGTGCTGAGCGGAGTCGTGCTGGCCGGGACGGGGACCGTCACGGGG
GTCTTCGTCGCCGTCGCCGTCAGCGTCGGTCCCGCCATGTCGGCGATGGGACGCGCCGAA
TACCTGCGGGCACACGCGCTGCTCGGCAAGGGGTACCACCCGCTGATGCCGATCCTGGTC
AACATCGTGACGCTGAGCGGGTTCGCCCTCGCGTTCCTCGCCGGGGCACCGGGGAATGTC
CTGTTCCCCCTCGCCTCGGTGCTGCTCATCGGCGTGCAGGCGGTCTCACACCTCGGCAAC
GTCCCGATCAACCGCTCGCTGAGCGGCCTCGCGGCCGCGGGGTCCTGGGTGGACCCCCGT
CCGCAGTGGCGCGCCTGGCACCGCCTGCGGACGGTCCTGGCGGCACTCGCCCTGGCCGCC
GTCACGGTCGCGGTGCTCACCGCCCGCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR013901 Protein of unknown function DUF1772 (Family)
[20-144] 1.3e-19 PF08592
PF08592   DUF1772
SignalP
[1-34] 0.5 Signal
Eukaryota   
[1-34] 0.843 Signal
Bacteria, Gram-positive   
[1-26] 0.505 Signal
Bacteria, Gram-negative   
TMHMM
[7-29] Transmembrane (i-o) [51-73] Transmembrane (o-i) [80-99] Transmembrane (i-o) [126-148] Transmembrane (o-i)
Transmembrane 4   
Fred_00140 Fred_00140   Fred_00160 Fred_00160
Page top