Fred_00150 Fred_00150   Fred_00170 Fred_00170

Fred_00160 : CDS information

close this sectionLocation

Organism
StrainATCC 49344
Entry nameFredericamycin
Contig
Start / Stop / Direction13,801 / 14,265 / + [in whole cluster]
13,801 / 14,265 / + [in contig]
Location13801..14265 [in whole cluster]
13801..14265 [in contig]
TypeCDS
Length465 bp (154 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.1 modification hydroxylation
Producthydroxylase
Product (GenBank)putative monooxygenase
GenefdmM1
Gene (GenBank)
EC number
Keyword
  • pentangular C-13
Note
Note (GenBank)
  • FdmM1
Reference
ACC
PmId
[16305230] Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from Streptomyces griseus. (J Am Chem Soc. , 2005)
[19620242] In vivo investigation of the roles of FdmM and FdmM1 in fredericamycin biosynthesis unveiling a new family of oxygenases. (J Biol Chem. , 2009)
comment
[PMID: 16305230](2005)
Fredericamycin(FDM) gene clusterの同定論文。

fdmM1: oxygenase

FdmJ, L, M, M1, P, Qはantibiotic monooxygenase domainを持つ。
(interpro:26.0でIPR007138 Antibiotic biosynthesis monooxygenase domainが確認できるのはFdmJ, P, Qのみ）

fdmC-fdmT3のfragmentをS.albusへ導入し、FDM-A産生に十分であることを確認している。

---
[PMID: 19620242](2009)
delta-fdmM, delta-fdmM1 mutantを作成し産物解析。
FDM生合成においてFdmMはC-6、FdmM1はC-8での hydroxylationsを触媒する。
これらgenesのhomologはこれまで機能不明タンパクとされており、新しいoxygenase familyといえる。

close this sectionSequence

selected fasta
>hydroxylase [putative monooxygenase]
MAWEQVLMPPALLAGGLAAGGLAISVLAAPLFTTLPTVMYVPVHQGLVTRFDPFMPVSLA
SCLFCDAALVLAAQGGAVRLLAAAAALLLACAMTVSLTKNVPINRWLATLDPRALPADFQ
RRDPRTRWIRWNRVRGALAVAALVANVAAAALLI
selected fasta
>hydroxylase [putative monooxygenase]
GTGGCCTGGGAACAGGTACTCATGCCGCCGGCGCTGCTCGCCGGCGGGCTCGCGGCCGGT
GGCCTGGCGATCTCGGTGCTGGCCGCGCCGTTGTTCACCACCCTCCCGACCGTGATGTAC
GTCCCGGTCCACCAGGGCCTGGTCACCCGCTTCGATCCGTTCATGCCCGTGTCGCTGGCG
AGCTGCCTGTTCTGCGACGCCGCCCTGGTCCTTGCGGCCCAGGGCGGGGCGGTCCGGCTG
CTGGCCGCGGCGGCGGCGCTGCTGCTGGCCTGCGCCATGACGGTGTCGCTCACCAAGAAC
GTGCCGATCAACCGGTGGCTGGCGACCCTCGACCCGCGGGCGCTCCCCGCCGACTTCCAG
CGACGCGACCCCCGCACACGGTGGATCCGCTGGAACCGCGTACGGGGCGCTCTCGCGGTG
GCGGCGCTGGTGGCCAACGTCGCGGCGGCCGCGCTTCTCATCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR013901 Protein of unknown function DUF1772 (Family)
[22-149] 1.7e-16 PF08592
PF08592   DUF1772
SignalP
[1-28] 0.992 Signal
Bacteria, Gram-negative   
[1-28] 0.977 Signal
Eukaryota   
[1-28] 0.984 Signal
Bacteria, Gram-positive   
TMHMM
[12-34] Transmembrane (i-o) [75-97] Transmembrane (o-i) [131-153] Transmembrane (i-o)
Transmembrane 3   
Fred_00150 Fred_00150   Fred_00170 Fred_00170
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