Fred_00270 Fred_00270   Fred_00290 Fred_00290

Fred_00280 : CDS information

close this sectionLocation

Organism
StrainATCC 49344
Entry nameFredericamycin
Contig
Start / Stop / Direction26,150 / 26,629 / + [in whole cluster]
26,150 / 26,629 / + [in contig]
Location26150..26629 [in whole cluster]
26150..26629 [in contig]
TypeCDS
Length480 bp (159 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.4 Other
Productholo-[acyl-carrier-protein] synthase
4'-phosphopantetheinyl transferase AcpS
Product (GenBank)holo-ACP synthase
GenefdmW
Gene (GenBank)
EC number2.7.8.7
Keyword
Note
Note (GenBank)
  • FdmW
Reference
ACC
PmId
[16305230] Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from Streptomyces griseus. (J Am Chem Soc. , 2005)
[16895912] A dedicated phosphopantetheinyl transferase for the fredericamycin polyketide synthase from Streptomyces griseus. (J Biol Chem. , 2006)
comment
[PMID: 16305230](2005)
Fredericamycin(FDM) gene clusterの同定論文。

fdmW: holo-ACP synthase

FdmWは、resistomycin生合成gene clusterのputative ACPS-type PPTase=RemDにとても似ている。
apo-ACP FdmH の転写後修飾のためのPPTasesをたぶんコードする。

fdmC-fdmT3のfragmentをS.albusへ導入し、FDM-A産生に十分であることを確認している。

---
[PMID: 16895912](2006)
fdmWの不活化はFDM産生を約93%減少、相補で回復。
fdm clusterのACP(fdmH)と他のACPsとの反応との比較から、FdmWがFDM生合成に特異的であるといえる。（他のACPsでも全くphosphopantetheinylateできないわけではない。）

FdmWとの酵素活性
FdmH ACP, with a K(m) of 5.8 microM and a k(cat)/K(m) of 8.1 microM(-1) x min(-1)
TcmM ACP with a K(m) of 1.0 x 10(2) microM and a k(cat) /K(m) of 0.6 microM(-1) x min(-1)

他のPPTasesとのalignment結果からN末37aa truncated FdmWを作成して活性測定し、PPTase活性にはこのN末部分が必要であることを確認している。

close this sectionSequence

selected fasta
>holo-[acyl-carrier-protein] synthase [holo-ACP synthase]
MQPDDEGAREWRRGPGGSGRFPWPLRLRARHPRPAPPRIVGVGIDVAAIARFGLALERSP
GLRDRLFTPEEQMLPSGSPRTTASLAARFAAKEAVAKVLGAPGGLRWHDVGVRTGARGRP
VLQVCGTVAAAAARQGISVWHLSLTHDGDVASAVVVGAA
selected fasta
>holo-[acyl-carrier-protein] synthase [holo-ACP synthase]
ATGCAACCTGACGACGAGGGCGCGAGGGAGTGGCGGCGCGGGCCCGGCGGGAGTGGCCGG
TTCCCCTGGCCGCTGCGGCTGAGGGCGCGTCACCCGAGACCGGCGCCGCCGCGGATCGTG
GGTGTGGGGATCGACGTGGCGGCGATAGCCAGGTTCGGGCTGGCCCTGGAGCGCAGCCCC
GGGCTGAGGGACCGCCTGTTCACTCCGGAGGAGCAGATGCTGCCTTCGGGGTCGCCCCGG
ACGACGGCTTCCCTGGCCGCGCGATTCGCCGCCAAGGAGGCCGTCGCCAAGGTGCTCGGT
GCGCCGGGCGGGCTCCGCTGGCACGACGTGGGGGTGCGGACCGGCGCCCGCGGCCGGCCC
GTCCTCCAGGTGTGCGGCACGGTCGCGGCCGCCGCCGCCCGGCAGGGGATCTCGGTCTGG
CACCTGTCGCTCACCCATGACGGTGACGTCGCCTCCGCCGTCGTCGTCGGTGCGGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR004568 Phosphopantethiene-protein transferase (Domain)
[39-157] 3.39999999999999e-20 TIGR00556
TIGR00556   Pantethn_trn
[45-122] PD004282
PD004282   PPantethiene-prot_Trfase
IPR008278 4'-phosphopantetheinyl transferase (Domain)
[38-156] 3.69999438558106e-23 SSF56214
SSF56214   4-PPT_transf
[39-156] 1e-35 G3DSA:3.90.470.20
G3DSA:3.90.470.20   G3DSA:3.90.470.20
[41-155] 3.4e-15 PF01648
PF01648   ACPS
SignalP
[1-1] Signal-anchor
Eukaryota   
TMHMM No significant hit
Fred_00270 Fred_00270   Fred_00290 Fred_00290
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