Fred_00280 Fred_00280   Fred_00300 Fred_00300

Fred_00290 : CDS information

close this sectionLocation

Organism
StrainATCC 49344
Entry nameFredericamycin
Contig
Start / Stop / Direction26,869 / 27,381 / + [in whole cluster]
26,869 / 27,381 / + [in contig]
Location26869..27381 [in whole cluster]
26869..27381 [in contig]
TypeCDS
Length513 bp (170 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
Productputative MarR family transcriptional regulator
Product (GenBank)transcriptional regulator
GenefdmR2
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)
  • FdmR2
Reference
ACC
PmId
[16305230] Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from Streptomyces griseus. (J Am Chem Soc. , 2005)
[18556785] Identification and utility of FdmR1 as a Streptomyces antibiotic regulatory protein activator for fredericamycin production in Streptomyces griseus ATCC 49344 and heterologous hosts. (J Bacteriol. , 2008)
comment
[PMID: 16305230](2005)
Fredericamycin(FDM) gene clusterの同定論文。

fdmR2: regulator

RubM, GrhR3に似ている。
transcriptional regulatorsのMarR-familyに属する。

fdmC-fdmT3のfragmentをS.albusへ導入し、FDM-A産生に十分であることを確認している。

---
[PMID: 18556785](2008)
メインはSARP-type activator_FdmR1の機能解析。

fdmR2はputative SARP binding site-containing promotersの下流にある。
fdmR1不活化mutantでfdmR2転写物は減少するので、FdmR1によって少なくとも部分的に調節され、他のregulatorsによって活性化されるかもしれない。

fdm生合成gene clusterの場合、FdmR1は直接的に生合成遺伝子を活性化し、部分的にputative repressor geneであるfdmR2を調節する。FdmR2はFDMsの毒性レベルの蓄積を避けるためfeedback controlとして働くかもしれない。
Family/Domain
FD
IPR000835
comment
[IPR000835] HTH transcriptional regulator, MarR(family)
function; sequence-specific DNA binding transcription factor activity
all signatures hit

close this sectionSequence

selected fasta
>putative MarR family transcriptional regulator [transcriptional regulator]
MERTTHTAGGDTAARKASPALPSGSLAQDLALVLPALHRALDRRVDADFPHPKPAEGQIA
LMRLIRDREGVTVREAAEVLLVKPNNLSATVTQMVAAGLVERRRDPDDKRVTHLHLTPTA
AERTHAVDELIGGYVEQALAGLSDGERGALGAAVGALHLLERGLHPSSAS
selected fasta
>putative MarR family transcriptional regulator [transcriptional regulator]
GTGGAGAGAACGACACATACGGCGGGCGGCGACACGGCAGCGCGGAAGGCGAGCCCTGCC
CTGCCCTCCGGCTCGCTCGCCCAGGACCTCGCCCTCGTCCTCCCCGCCCTCCACCGCGCC
CTGGACCGCCGCGTGGACGCCGACTTCCCGCACCCCAAGCCCGCCGAGGGCCAGATCGCC
CTGATGCGGCTGATCCGGGACCGGGAGGGCGTCACCGTCCGGGAGGCGGCCGAGGTGCTG
CTGGTCAAGCCCAACAACCTCAGCGCGACGGTGACCCAGATGGTGGCCGCGGGGCTGGTC
GAGCGCCGCCGGGACCCGGACGACAAGCGCGTCACCCACCTCCACCTCACCCCGACCGCA
GCCGAACGCACGCACGCCGTCGACGAGTTGATCGGCGGGTACGTCGAGCAGGCCCTGGCC
GGCCTCAGCGACGGCGAGCGGGGCGCACTCGGCGCCGCCGTCGGAGCCCTGCACCTGCTG
GAGCGCGGCCTCCACCCGTCGTCCGCCTCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000835 Transcription regulator HTH, MarR-type (Family)
[27-159] PS50995
PS50995   HTH_MARR_2
[47-147] 3.80000697093586e-17 SM00347
SM00347   HTH_MARR
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[26-157] 1.9e-25 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
IPR023187 Transcriptional regulator MarR-type, conserved site (Conserved_site)
[86-120] PS01117
PS01117   HTH_MARR_1
SignalP
[1-40] 0.105 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Fred_00280 Fred_00280   Fred_00300 Fred_00300
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