Fred_00300 : CDS information

Location

Organism	Streptomyces griseus
Strain	ATCC 49344
Entry name	Fredericamycin
Contig	AF525490
Start / Stop / Direction	27,689 / 28,906 / + [in whole cluster] 27,689 / 28,906 / + [in contig]
Location	27689..28906 [in whole cluster] 27689..28906 [in contig]
Type	CDS
Length	1,218 bp (405 aa)

Annotation

Category	5.1 general function
Product	putative transposase
Product (GenBank)	transposase
Gene	fdmX
Gene (GenBank)
EC number
Keyword
Note
Note (GenBank)	FdmX
Reference	ACC Q2MGA9 PmId [16305230] Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from Streptomyces griseus. (J Am Chem Soc. , 2005) comment Fredericamycin(FDM) gene clusterの同定論文。 fdmX: transposase SC3C8.10, S0055に似ている。 transposasesに似ているが、FDM A生合成における役割は不明。 fdmC-fdmT3のfragmentをS.albusへ導入し、FDM-A産生に十分であることを確認している。
Family/Domain	FD IPR003346 comment [IPR003346] Transposase, IS116/IS110/IS902 (Family) function; DNA binding, transposase activity

Sequence

>putative transposase [transposase]
MFEIEDVGVFLGLDVGKTAHHGHGLTPAGKKIFDKPLPNSEPKLRAVFDKLTEKFGTVLV
IVDQPASIGALPLTVARDAGCRVAYLPGLAMRRIADLYPGEAKTDAKDAAVIADAARTMP
HTLRCLELTDEITAELTVLTGFDQDLAAEATRTSNRIRGLLTQFHPSLERVLGPRLDHQA
VTWLLERHGSPAALRKAGRRRLVELIRPKAPRMAQRLIDDVFDALDEQTVVVPGTGTLDT
VVPSLAASLASVHTQRRAMEAQINTLLEAHPLSPVLTSMPGVGVRTAAVLLVTVGDGTSF
PTAAHLASYAGLAPTTKSSGTSIHGEHAPRGGNRQLKRAMFLSAFACMNADPASRTYYDR
QRARGKTHTQALLRLARQRISVLFAMLRDGTFYEPRTPKNVELAA

>putative transposase [transposase]
ATGTTCGAGATCGAAGACGTGGGCGTGTTCCTCGGCCTGGACGTCGGCAAAACCGCTCAC
CACGGCCACGGACTCACCCCGGCCGGGAAGAAAATCTTCGACAAACCGCTACCCAACAGC
GAGCCGAAACTGCGGGCCGTCTTCGACAAGCTGACCGAGAAGTTCGGCACCGTCCTGGTC
ATCGTGGACCAGCCCGCCTCCATCGGCGCCCTCCCGCTGACCGTCGCCCGCGACGCCGGC
TGCCGCGTCGCCTACCTGCCCGGCCTGGCCATGCGCCGCATCGCCGACCTCTACCCCGGC
GAGGCCAAGACCGACGCCAAAGACGCCGCCGTCATCGCCGACGCCGCACGGACCATGCCG
CACACCCTGCGCTGCCTGGAACTCACCGACGAGATCACCGCCGAACTCACCGTGCTGACC
GGCTTCGACCAGGACCTCGCCGCCGAAGCCACCCGCACCTCCAACCGGATACGCGGCCTG
CTCACCCAGTTCCACCCCAGCCTCGAACGCGTCCTGGGCCCCCGCCTGGACCACCAGGCC
GTCACCTGGCTCCTGGAACGTCACGGCTCCCCGGCCGCCCTGCGCAAGGCTGGCCGCCGC
CGGCTCGTCGAACTGATCCGCCCCAAGGCCCCGCGCATGGCACAGCGCCTAATCGACGAC
GTCTTCGACGCCCTCGACGAGCAGACCGTCGTCGTCCCCGGCACCGGCACCCTCGACACC
GTCGTGCCCTCCCTGGCCGCTTCGCTGGCCTCCGTCCACACCCAGCGCCGTGCCATGGAA
GCCCAGATCAACACCCTGCTGGAGGCCCACCCTCTTTCCCCGGTCCTGACGTCGATGCCC
GGCGTCGGCGTCAGGACCGCCGCAGTCCTACTGGTCACCGTAGGCGACGGCACCAGCTTC
CCCACCGCCGCCCACCTCGCCTCCTACGCCGGCCTCGCCCCGACCACGAAGTCGTCCGGG
ACCTCGATCCACGGCGAACACGCACCCCGAGGCGGAAACCGGCAGCTCAAACGCGCCATG
TTCCTCTCCGCCTTCGCCTGCATGAACGCCGACCCCGCCTCCCGCACCTACTACGACCGC
CAGCGAGCCCGCGGCAAAACCCACACCCAAGCCCTCCTCCGCCTCGCTCGCCAACGCATC
AGCGTCCTGTTCGCCATGCTCCGCGACGGCACCTTCTACGAACCCCGGACACCGAAAAAC
GTCGAGCTCGCCGCATAA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002525 Transposase, IS111A/IS1328/IS1533, N-terminal (Domain) PF01548 DEDD_Tnp_IS110 IPR003346 Transposase, IS116/IS110/IS902 (Family) PF02371 Transposase_20
SignalP	No significant hit
TMHMM	No significant hit

Fred_00290 Fred_00310