Fred_00320 : CDS information

Location

Organism	Streptomyces griseus
Strain	ATCC 49344
Entry name	Fredericamycin
Contig	AF525490
Start / Stop / Direction	31,229 / 30,621 / - [in whole cluster] 31,229 / 30,621 / - [in contig]
Location	complement(30621..31229) [in whole cluster] complement(30621..31229) [in contig]
Type	CDS
Length	609 bp (202 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	unknown
Gene
Gene (GenBank)
EC number
Keyword
Note	It is supposed that this ORF is not involved in fredericamycins biosynthesis.
Note (GenBank)	Orf32
Reference	ACC Q2MGA7 PmId [16305230] Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from Streptomyces griseus. (J Am Chem Soc. , 2005) comment Fredericamycin(FDM) gene clusterの同定論文。 Orf32: unknown function fdm clusterの境界決定のため、orf1, 3, 33を遺伝子置換により不活化。これらのmutantはFDM-A産生にあまり影響しない。さらにこれらのgenesを除外したfdmC-fdmT3(orf32は含まれない)のfragmentをS.albusへ導入し、FDM-A産生に十分であることを確認している。

Sequence

>hypothetical protein [unknown]
MTTSTAPTEPGTPENLTARRVTDVLQPRNVLLGGMLGIGLAAAGGHWSGLLWGFLGALCA
GLIPAGYIEWQRGRGAWGDRHVVDRTKRAPIFFVILGSIGAGSLVMVAGNAPRDILVTML
ALWAMTIVLLAVNTVWKISVDAAVASAVVAQLAVVHHPAWLAAYALAGAVCWSRVALGYH
TVGQVCAGTAAGVATAAGFLVG

>hypothetical protein [unknown]
ATGACCACCAGCACCGCCCCCACCGAGCCCGGCACGCCGGAGAACCTCACCGCCCGCCGC
GTCACCGACGTCCTCCAGCCCCGCAACGTCCTCCTCGGCGGGATGCTCGGCATCGGCCTC
GCGGCGGCCGGCGGACACTGGTCCGGCCTCCTCTGGGGGTTCCTCGGGGCACTCTGCGCC
GGCCTCATACCCGCCGGATACATCGAATGGCAGCGCGGACGCGGCGCCTGGGGCGACCGC
CACGTGGTCGACCGCACCAAGCGCGCGCCGATCTTCTTCGTCATCCTCGGCTCCATCGGC
GCCGGTTCCCTGGTCATGGTGGCCGGGAACGCCCCCCGCGACATCCTCGTCACCATGCTG
GCCCTCTGGGCCATGACCATCGTCCTGCTCGCCGTCAACACCGTCTGGAAGATCTCCGTC
GACGCCGCCGTCGCCTCGGCCGTGGTGGCCCAGCTGGCCGTGGTGCACCATCCGGCGTGG
CTCGCCGCCTACGCGCTGGCGGGGGCGGTGTGCTGGTCGCGGGTGGCGCTGGGGTACCAC
ACGGTGGGGCAGGTGTGCGCGGGGACGGCGGCGGGGGTGGCTACTGCTGCGGGGTTCCTG
GTGGGGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro	No significant hit
SignalP	Eukaryota
TMHMM	Transmembrane 5

Fred_00310 Fred_00330