Fred_00330 : CDS information

Location

Organism	Streptomyces griseus
Strain	ATCC 49344
Entry name	Fredericamycin
Contig	AF525490
Start / Stop / Direction	32,515 / 31,226 / - [in whole cluster] 32,515 / 31,226 / - [in contig]
Location	complement(31226..32515) [in whole cluster] complement(31226..32515) [in contig]
Type	CDS
Length	1,290 bp (429 aa)

Annotation

Category	5.4 hypothetical protein
Product	hypothetical protein
Product (GenBank)	unknown
Gene
Gene (GenBank)
EC number
Keyword
Note	This ORF is not involved in fredericamycins biosynthesis.
Note (GenBank)	Orf33
Reference	ACC Q2MGA6 PmId [16305230] Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from Streptomyces griseus. (J Am Chem Soc. , 2005) comment Fredericamycin(FDM) gene clusterの同定論文。 Orf33: unknown function fdm clusterの境界決定のため、orf1, 3, 33を遺伝子置換により不活化。これらのmutantはFDM-A産生にあまり影響しない。さらにこれらのgenesを除外したfdmC-fdmT3のfragmentをS.albusへ導入し、FDM-A産生に十分であることを確認している。

Sequence

>hypothetical protein [unknown]
MSTLDLAGYLVAVLMTAVALWRMPAALWGDEEDRRRRALWGCYAGFAAALWTKTEFVRTT
LNDSPVTDLAVLIKHYTATIAILAILSYIVAIYGRYPDRGAVPRHVRFARLVQRVAAKAS
VATLILLTVLFFTVVDRSVPSDRFVSDHAGQPGATLYMTVFYVYLGAASAVCAYQWKLAT
ADATARHLRVGLSMMTAAMFIGVAYTASRTLFMWISVVDQPSVEFADTFDKVTEAGQVLL
FVLFAVGASLPAFSTGLRRARLWRAQARLHPLWRELMTAFPEQPFAPPASLLREVTRFDT
PADLRVDRWSADIADAVEKLRHYAPGHLADAARTAAAEDTADPDERGPRTDAHWIRAALV
AHAEGVPAGPAAPATDPRQAEDQEGEVAWLVRVAAACRTIPAARTTAVLAAARAGEAAAT
TNSPEQPTR

>hypothetical protein [unknown]
GTGAGTACCCTCGATCTGGCCGGTTACCTCGTCGCCGTGCTGATGACGGCCGTCGCCCTG
TGGCGGATGCCGGCGGCCCTCTGGGGGGACGAGGAGGACCGCCGCAGACGCGCCCTGTGG
GGCTGCTACGCCGGATTCGCCGCCGCCCTGTGGACCAAGACCGAGTTCGTCCGCACCACG
CTCAACGACAGCCCGGTCACCGACCTCGCCGTCCTCATCAAGCACTACACGGCGACCATC
GCGATCCTCGCCATCCTCAGCTACATCGTCGCCATCTACGGCCGCTACCCGGACCGGGGC
GCCGTCCCCCGCCACGTCCGCTTCGCCCGCCTCGTGCAGCGCGTCGCCGCCAAGGCGTCG
GTCGCCACCCTCATCCTGCTGACCGTCCTCTTCTTCACCGTCGTCGACCGCTCGGTCCCC
TCCGACCGCTTCGTCTCCGACCACGCGGGCCAGCCCGGCGCCACCCTCTACATGACGGTC
TTCTACGTCTACCTCGGCGCCGCCAGTGCCGTCTGCGCCTACCAGTGGAAGCTGGCCACC
GCCGACGCCACCGCCCGCCACCTGCGCGTCGGCCTGTCGATGATGACCGCCGCCATGTTC
ATCGGCGTCGCCTACACCGCCAGCCGCACCCTGTTCATGTGGATCAGCGTCGTCGACCAG
CCGAGCGTGGAGTTCGCCGACACCTTCGACAAGGTCACCGAGGCCGGACAGGTCCTGCTC
TTCGTCCTGTTCGCCGTCGGCGCCTCGCTGCCCGCCTTCTCCACCGGCCTGCGCCGCGCC
CGCCTCTGGCGCGCCCAGGCCCGGCTGCACCCGCTCTGGCGCGAGCTGATGACCGCCTTC
CCCGAGCAGCCCTTCGCCCCGCCCGCCTCCCTGCTGCGCGAGGTCACCCGCTTCGACACC
CCGGCCGACCTGCGCGTCGACCGCTGGTCCGCCGACATCGCCGACGCCGTCGAGAAGCTG
CGCCACTACGCGCCCGGGCACCTGGCCGACGCCGCCCGCACCGCCGCCGCCGAGGACACC
GCCGACCCCGACGAGCGGGGCCCGCGCACCGACGCCCACTGGATACGGGCCGCCCTCGTC
GCCCACGCCGAGGGCGTACCCGCCGGGCCCGCCGCCCCCGCCACCGACCCACGGCAGGCC
GAGGACCAGGAGGGCGAGGTCGCCTGGCTCGTCCGGGTCGCCGCCGCCTGCCGCACCATC
CCCGCCGCCCGCACCACCGCCGTCCTCGCCGCCGCCCGCGCGGGCGAGGCCGCCGCCACC
ACGAACTCCCCGGAGCAGCCGACCCGATGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro	No significant hit
SignalP	Bacteria, Gram-positive Eukaryota Bacteria, Gram-negative
TMHMM	Transmembrane 6

Fred_00320 last last