Gilvo_00040 : CDS information

Location

Organism	Streptomyces griseoflavus
Strain	Gö 3592
Entry name	Gilvocarcin
Contig	AY233211
Start / Stop / Direction	7,769 / 6,573 / - [in whole cluster] 7,769 / 6,573 / - [in contig]
Location	complement(6573..7769) [in whole cluster] complement(6573..7769) [in contig]
Type	CDS
Length	1,197 bp (398 aa)

Annotation

Category	3.3 modification reduction
Product	cytochrome P450
Product (GenBank)	putative cytochrome P-450 monooxygenase
Gene
Gene (GenBank)	gilOIII
EC number
Keyword	C-1'' hydroxylate vinyl group formation
Note
Note (GenBank)	GilOIII
Reference	ACC Q7X2G4 PmId [12822997] The complete gene cluster of the antitumor agent gilvocarcin V and its implication for the biosynthesis of the gilvocarcins. (J Am Chem Soc. , 2003) [16795121] Inactivation of gilGT, encoding a C-glycosyltransferase, and gilOIII, encoding a P450 enzyme, allows the details of the late biosynthetic pathway to gilvocarcin V to be delineated. (Chembiochem. , 2006) comment [PMID: 12822997](2003) gilvocarcin(gil) gene cluster同定のためにクローニングされたcos-G9B3をS.lividans TK24に導入すると、wildレベルのgilvocarcins V and M産生あり。よってgilvocarcin生合成のための完全なclusterが含まれている。 cos-G9B3をサブクローニングしてシークエンス。32.9kbpに26ORFsあり。 supporting info. Table 1 GilOIII (398aa): Cytochrome P450 oxygenase 配列解析のみ。1''-positionのHがP450 hydroxylase_GilOIIIでOH基となり、それが隣のmethyl基のHと共にdehydrationを受けてvinyl基を形成すると提唱されている。 --- [PMID: 16795121](2006) gilOIIIのdeletion mutantは、gilvocarcin E (gilvocarcin Vのvinyl基部分が-CH2-CH3)を蓄積。よってGilOIII はgilvocarcin Vのvinyl基形成に関連するP450 enzymeである。しかしgilvocarcin Eはgilvocarcin V生合成の中間体ではなく、shunt産物であり、生合成経路においてvinyl基形成が起こる順番は確定できない。

Sequence

>cytochrome P450 [putative cytochrome P-450 monooxygenase]
MISTSTIPHLDLDPYTDASITDPYPLYGALRDAAPVVYLTAHQVHCAARYDEVYAALHRH
GTFVSGEGVGLTDTLNKAQKGSSFTSDPPYHAYVRGLVARHLKPRAMADINGYVTSWADR
LVRDLVARKSFDAVQDFAVAFPLAVVPDLLGWPEGEGKERLLEWASAGFNAFGPLNDRTR
AGLPLLGEMGEFLHRMSQPGNLRPGSWGAELVSEAREGKIEDKLLPGLLGDFLAPSMDTS
VSALSSMLWLLGTHQDQWRRLREDPSLVSSAFNEIIRLESPLRGFTRYVAEDTELGGAAL
PRGSRVLLLFGSANRDERFWKDPESFDVARQGAAQHVGFGHGIHACVGQALSRLEGQSLL
EALVRHVDRIEVGEPTWRLHNTIRGIERMDVTFHGRTA

>cytochrome P450 [putative cytochrome P-450 monooxygenase]
GTGATCTCCACATCGACGATCCCCCACCTCGATCTCGATCCGTACACGGACGCGTCGATA
ACCGACCCGTACCCCCTGTACGGTGCGCTGCGCGACGCGGCACCGGTGGTGTACCTGACG
GCGCACCAGGTGCACTGCGCCGCCCGCTACGACGAGGTGTACGCGGCCCTGCACCGCCAC
GGCACGTTCGTGTCCGGCGAGGGCGTCGGTCTGACCGACACCCTGAACAAGGCGCAGAAG
GGCAGTAGCTTCACCAGCGACCCGCCGTACCACGCCTACGTGCGGGGGCTCGTGGCCCGC
CACCTGAAGCCCCGCGCGATGGCCGACATCAACGGGTACGTCACGTCCTGGGCGGACCGT
CTTGTCCGGGACCTCGTCGCCCGGAAGTCGTTCGACGCCGTCCAGGACTTCGCGGTCGCC
TTCCCGCTGGCCGTGGTGCCCGATCTGCTGGGATGGCCGGAGGGCGAGGGGAAGGAAAGG
CTCCTGGAGTGGGCCAGCGCCGGATTCAACGCCTTCGGTCCCCTGAACGACCGGACGCGG
GCCGGCCTGCCGCTGCTCGGCGAGATGGGCGAGTTCCTCCACCGGATGTCACAGCCCGGC
AATCTGCGTCCGGGGAGCTGGGGTGCCGAACTCGTCTCGGAGGCCCGCGAGGGCAAGATC
GAGGACAAGCTGCTGCCCGGACTGCTCGGTGACTTCCTCGCCCCGTCCATGGACACGTCC
GTCAGCGCGCTCTCCAGCATGCTGTGGCTGCTCGGCACGCACCAGGACCAGTGGCGCCGT
CTGCGGGAGGACCCCTCCCTGGTCTCGTCGGCCTTCAACGAGATCATCCGGCTGGAATCG
CCGCTGCGCGGGTTCACCCGCTACGTCGCCGAGGACACGGAGCTGGGCGGAGCCGCGCTG
CCCCGCGGATCGCGCGTCCTCCTCCTCTTCGGTTCGGCCAACCGTGACGAGCGGTTCTGG
AAGGACCCGGAGTCCTTCGACGTCGCCCGGCAGGGCGCGGCCCAGCACGTCGGTTTCGGG
CACGGTATCCACGCCTGCGTCGGTCAGGCCCTGTCCCGCCTGGAGGGGCAGAGCCTCCTG
GAGGCGCTGGTCCGTCACGTCGACAGGATCGAGGTGGGGGAGCCGACCTGGAGGCTGCAC
AACACCATCCGCGGGATCGAGAGGATGGATGTGACGTTCCATGGACGAACTGCCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001128 Cytochrome P450 (Family) SSF48264 Cytochrome_P450 G3DSA:1.10.630.10 Cyt_P450 PF00067 p450 IPR002397 Cytochrome P450, B-class (Family) PR00359 BP450 IPR017972 Cytochrome P450, conserved site (Conserved_site) PS00086 CYTOCHROME_P450
SignalP	No significant hit
TMHMM	No significant hit

Gilvo_00030 Gilvo_00050