Gilvo_00170 Gilvo_00170   Gilvo_00190 Gilvo_00190

Gilvo_00180 : CDS information

close this sectionLocation

Organism
StrainGö 3592
Entry nameGilvocarcin
Contig
Start / Stop / Direction21,735 / 22,994 / + [in whole cluster]
21,735 / 22,994 / + [in contig]
Location21735..22994 [in whole cluster]
21735..22994 [in contig]
TypeCDS
Length1,260 bp (419 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase beta-ketoacyl synthase subunit
Product (GenBank)putative alpha-ketoacyl synthase
Gene
Gene (GenBank)gilA
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
  • GilA
Reference
ACC
PmId
[12822997] The complete gene cluster of the antitumor agent gilvocarcin V and its implication for the biosynthesis of the gilvocarcins. (J Am Chem Soc. , 2003)
[20486694] Enzymatic total synthesis of rabelomycin, an angucycline group antibiotic. (Org Lett. , 2010)
comment
[PMID: 12822997](2003)
gilvocarcin(gil) gene cluster同定のためにクローニングされたcos-G9B3をS.lividans TK24に導入すると、wildレベルのgilvocarcins V and M産生あり。よってgilvocarcin生合成のための完全なclusterが含まれている。

cos-G9B3をサブクローニングしてシークエンス。32.9kbpに26ORFsあり。
supporting info. Table 1
GilA(419aa): Ketosynthase KS alpha

配列解析のみ

---
[PMID: 20486694](2010)
GilAB, RavC, GilP, GilF, RavG, JadD + acetyl-CoA, malonyl-CoAのmixtureでrabelomycinが産生されることを確認。
acetyl-CoA or malonyl-CoAのどちらかが除かれたとき、rabelomycinの産生は完全に廃止された。

close this sectionPKS/NRPS Module

KS3..372

close this sectionSequence

selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [putative alpha-ketoacyl synthase]
MSRRVFITGVGVVAPGAVGRDPFWELLTQGRTATRRLSLCDPEPFRSQVAAEADFDAEAA
GLSERQSAELDRAAQFALVAAREAVEDAAWSETCPPERAGVIVGSAVGATTKLEEVYRQL
SRDGSLWDVAPDSPAELYSYFVPSSFASGIAHDLGVTGQSGVVSTGCTSGIDSVGNAWEL
IQSGILDSAVCGATDAPISPITVACFDTIKATSTYNDTPESASRPFDATRGGFVLGEGSA
MFVLESEESVHRRGARVYGEIRGYASRCNAYHMTGLKADGRELAEAVVSALGQAGVDPGR
LDYVNAHGSGTKQNDRHETAALKSSLGPAAHDVPISSIKSMIGHSLGAIGSLEIAACALA
LRDDVIPPTANLTRPDPELDLDYVPVHARKQPTNSVLTTGSGFGGFQSAMVLTDPEHHS
selected fasta
>polyketide synthase beta-ketoacyl synthase subunit [putative alpha-ketoacyl synthase]
ATGAGCCGCAGGGTCTTCATCACCGGGGTCGGTGTCGTCGCGCCGGGAGCCGTCGGACGT
GACCCCTTCTGGGAGCTGCTGACCCAAGGGCGCACGGCCACCCGCCGGCTCAGCCTCTGC
GACCCGGAGCCCTTCCGGTCCCAGGTGGCCGCGGAGGCCGACTTCGACGCCGAGGCGGCG
GGGCTGTCGGAGCGGCAGTCCGCGGAACTGGACCGGGCGGCGCAGTTCGCCCTGGTCGCC
GCCCGTGAAGCGGTCGAGGACGCGGCATGGTCCGAGACATGTCCTCCCGAACGCGCCGGA
GTGATCGTGGGTTCGGCCGTCGGAGCCACGACCAAGCTCGAGGAGGTCTACCGGCAGCTC
AGCCGTGACGGCTCCCTCTGGGACGTGGCCCCCGACTCCCCCGCCGAGCTGTACTCGTAC
TTCGTGCCCAGCTCGTTCGCCTCCGGCATCGCACACGACCTCGGCGTCACGGGGCAGAGC
GGCGTCGTGTCGACCGGGTGCACCTCCGGGATCGACTCCGTCGGCAACGCCTGGGAACTG
ATCCAGAGCGGCATCCTGGACTCCGCCGTCTGCGGTGCCACCGACGCCCCCATCTCGCCC
ATCACCGTCGCCTGCTTCGACACGATCAAGGCGACATCGACGTACAACGACACCCCGGAG
AGCGCCTCACGGCCGTTCGACGCCACACGGGGCGGCTTCGTCCTCGGCGAGGGCAGCGCG
ATGTTCGTCCTCGAATCGGAGGAATCCGTCCACCGTCGCGGCGCACGCGTCTACGGCGAG
ATCCGCGGCTACGCGAGCCGCTGCAACGCCTACCACATGACCGGTCTCAAGGCCGACGGA
CGCGAGCTGGCGGAGGCCGTCGTCTCCGCTCTCGGCCAGGCAGGCGTGGACCCGGGCCGG
CTCGACTACGTCAACGCCCACGGCAGCGGCACGAAGCAGAACGACCGCCACGAGACCGCC
GCGCTGAAGTCGTCCCTCGGACCCGCCGCCCACGACGTGCCGATCAGTTCGATCAAGTCG
ATGATCGGCCATTCGCTGGGCGCCATCGGGTCGTTGGAGATCGCCGCCTGCGCCCTGGCG
CTGCGGGACGACGTGATCCCGCCGACGGCCAATCTCACCCGGCCGGATCCGGAACTCGAT
CTGGACTACGTGCCGGTCCACGCGCGCAAGCAGCCGACCAACAGCGTGCTCACGACCGGA
AGCGGCTTCGGTGGGTTTCAGAGCGCCATGGTTCTCACGGACCCGGAGCATCACTCATGA
KS3..372
KS7..1116

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR014030 Beta-ketoacyl synthase, N-terminal (Domain)
[3-248] 2.99999999999998e-51 PF00109
PF00109   ketoacyl-synt
IPR014031 Beta-ketoacyl synthase, C-terminal (Domain)
[258-372] 2.70000000000002e-37 PF02801
PF02801   Ketoacyl-synt_C
IPR016038 Thiolase-like, subgroup (Domain)
[7-263] 1.29999999999998e-59 G3DSA:3.40.47.10 [268-415] 4.39999999999997e-49 G3DSA:3.40.47.10
G3DSA:3.40.47.10   Thiolase-like_subgr
IPR016039 Thiolase-like (Domain)
[2-255] 4.69998262513195e-59 SSF53901 [217-416] 7.30000590915205e-62 SSF53901
SSF53901   Thiolase-like
IPR018201 Beta-ketoacyl synthase, active site (Active_site)
[158-174] PS00606
PS00606   B_KETOACYL_SYNTHASE
SignalP
[1-19] 0.97 Signal
Eukaryota   
[1-19] 0.582 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Gilvo_00170 Gilvo_00170   Gilvo_00190 Gilvo_00190
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