Gilvo_00190 Gilvo_00190   Gilvo_00210 Gilvo_00210

Gilvo_00200 : CDS information

close this sectionLocation

Organism
StrainGö 3592
Entry nameGilvocarcin
Contig
Start / Stop / Direction24,182 / 24,451 / + [in whole cluster]
24,182 / 24,451 / + [in contig]
Location24182..24451 [in whole cluster]
24182..24451 [in contig]
TypeCDS
Length270 bp (89 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category1.1 PKS
Productpolyketide synthase acyl carrier protein subunit
Product (GenBank)putative acyl carrier protein
Gene
Gene (GenBank)gilC
EC number
Keyword
  • type II PKS
Note
Note (GenBank)
  • GilC
Reference
ACC
PmId
[12822997] The complete gene cluster of the antitumor agent gilvocarcin V and its implication for the biosynthesis of the gilvocarcins. (J Am Chem Soc. , 2003)
comment
gilvocarcin(gil) gene cluster同定のためにクローニングされたcos-G9B3をS.lividans TK24に導入すると、wildレベルのgilvocarcins V and M産生あり。よってgilvocarcin生合成のための完全なclusterが含まれている。

cos-G9B3をサブクローニングしてシークエンス。32.9kbpに26ORFsあり。
supporting info. Table 1
GilC(89aa): Acyl carrier protein

配列解析のみ
Related Reference
ACC
Q56165
NITE
Jado_00100
PmId
[7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
Blast 2nd, id55%, 6e-17
Streptomyces venezuelae_acp(jadC)
Acyl carrier protein

--
[PMID:7881555](1994)
ORF3(=JadC)は配列解析からtype II PKSのACPであると提唱されている。
ORF2-ORF4領域を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。

--
[PMID: 9224566](1997)
minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。
jadCもACPとして使用されている。

close this sectionPKS/NRPS Module

ACP6..79

close this sectionSequence

selected fasta
>polyketide synthase acyl carrier protein subunit [putative acyl carrier protein]
MSARVTMDDLRRALEEGSGVDEGVDLDTDLETMAFSELGYDSLAVLETGLRLGRENDIEL
DDSVFADLDTPQQMLDAVNDALARQAAAS
selected fasta
>polyketide synthase acyl carrier protein subunit [putative acyl carrier protein]
ATGTCCGCACGCGTCACCATGGACGATCTCAGGCGAGCCCTCGAGGAGGGCTCCGGTGTC
GACGAGGGCGTCGATCTTGACACCGACCTCGAAACCATGGCGTTCTCCGAGCTGGGGTAC
GACTCCCTGGCGGTGCTGGAGACCGGCCTGCGCCTCGGCCGCGAGAACGACATCGAGCTC
GACGACTCGGTGTTCGCCGACCTCGACACGCCTCAGCAGATGCTGGACGCGGTCAACGAT
GCCCTCGCGCGTCAGGCGGCGGCATCGTGA
ACP6..79
ACP16..237

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR009081 Acyl carrier protein-like (Domain)
[6-83] 5.29999999999999e-13 G3DSA:1.10.1200.10
G3DSA:1.10.1200.10   ACP_like
[1-86] 8.49999291746323e-12 SSF47336
SSF47336   ACP_like
[10-78] 4.1e-07 PF00550
PF00550   PP-binding
[6-79] PS50075
PS50075   ACP_DOMAIN
SignalP No significant hit
TMHMM No significant hit
Gilvo_00190 Gilvo_00190   Gilvo_00210 Gilvo_00210
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