Gilvo_00210 : CDS information

Location

Organism	Streptomyces griseoflavus
Strain	Gö 3592
Entry name	Gilvocarcin
Contig	AY233211
Start / Stop / Direction	24,448 / 25,227 / + [in whole cluster] 24,448 / 25,227 / + [in contig]
Location	24448..25227 [in whole cluster] 24448..25227 [in contig]
Type	CDS
Length	780 bp (259 aa)

Annotation

Category	3.3 modification reduction
Product	putative polyketide C-9 ketoreductase
Product (GenBank)	putative ketoreductase
Gene
Gene (GenBank)	gilF
EC number
Keyword
Note
Note (GenBank)	GilF
Reference	ACC Q7X2G2 PmId [12822997] The complete gene cluster of the antitumor agent gilvocarcin V and its implication for the biosynthesis of the gilvocarcins. (J Am Chem Soc. , 2003) comment gilvocarcin(gil) gene cluster同定のためにクローニングされたcos-G9B3をS.lividans TK24に導入すると、wildレベルのgilvocarcins V and M産生あり。よってgilvocarcin生合成のための完全なclusterが含まれている。 cos-G9B3をサブクローニングしてシークエンス。32.9kbpに26ORFsあり。 supporting info. Table 1 GilF(256aa): C-9 ketoreductase 配列解析のみ
Related Reference	ACC Q8VWB5 PmId [8157619] Nucleotide sequence of the aknA region of the aklavinone biosynthetic gene cluster of Streptomyces galilaeus. (J Bacteriol. , 1994) comment Blast 14th, id65%, 2e-85 Streptomyces galilaeus(3AR-33)_aknA AknA 2-hydroxyaklavinone(polyketide C9の位置にOH基が残っているaklavinone)を産生するS. galilaeus ANR-58では、aknAの配列に変更がある。
Related Reference	ACC Q56166 NITE Jado_00110 PmId [7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994) [9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997) comment Blast 28th, id61%, 1e-82 Streptomyces venezuelae_ORF5(jadE) Ketoreductase -- [PMID:7881555](1994) ORF5(=jadEに相当)は配列解析からketoreductaseであると提唱されている。 ORF2-ORF4領域(ORF5も含まれる)を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。 -- [PMID: 9224566](1997) minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、そのplasmid発現株での産物を解析。 JadEはdecaketideだけでなく、octaketideも還元できる。

Sequence

>putative polyketide C-9 ketoreductase [putative ketoreductase]
MTSPRHALVTGGSSGIGKSVARRLASAGHTVTICGRDSERLQQAAKELSEQGAPVTSLIA
DVSKPRQVGDLVREAVETNGPLGILVNNAGRNGGGRTAELSDELWREVLSTNLDSVFYVT
REVLARGGIGEVDHARIINIASTAGKQGVLLAAPYSASKHGVVGFTKAVGKELAPQGITV
NAVCPGYVETPMASRVRQAYADAWETTEAEVLSAFEAKIPLGRYSTPDEVASLVEYLTTE
GAASITAQAFNVCGGLGNF

>putative polyketide C-9 ketoreductase [putative ketoreductase]
GTGACCTCTCCCCGTCATGCCCTGGTCACCGGCGGTTCCAGCGGCATAGGAAAGTCCGTC
GCACGGCGCCTGGCCTCGGCCGGCCACACCGTCACGATCTGCGGTCGTGACTCCGAAAGG
CTCCAGCAGGCCGCCAAGGAACTGTCGGAGCAGGGTGCACCCGTCACCTCGCTGATCGCC
GACGTCAGCAAGCCCCGCCAGGTGGGCGATCTGGTCCGCGAGGCCGTGGAGACGAACGGT
CCCCTCGGGATCCTCGTCAACAACGCGGGCAGGAACGGAGGCGGCCGGACCGCGGAGCTG
AGCGACGAGCTGTGGCGGGAGGTACTGAGCACCAACCTCGACAGCGTTTTCTACGTCACG
CGGGAGGTGCTGGCCCGTGGCGGCATCGGCGAGGTGGACCACGCCCGGATCATCAACATC
GCCTCCACCGCGGGGAAGCAGGGAGTTCTGCTGGCCGCCCCGTACTCCGCCTCCAAGCAC
GGTGTCGTCGGCTTCACCAAGGCGGTGGGCAAGGAGCTGGCCCCTCAGGGGATCACCGTG
AACGCCGTCTGCCCGGGCTACGTGGAGACCCCGATGGCCTCACGGGTCCGGCAGGCCTAC
GCAGACGCCTGGGAGACCACGGAGGCCGAGGTGCTGTCCGCCTTCGAGGCGAAGATCCCG
CTCGGCCGGTACAGCACGCCCGACGAGGTCGCCTCGCTGGTCGAGTACCTCACGACCGAA
GGAGCCGCCTCGATCACGGCTCAGGCGTTCAACGTGTGCGGCGGCCTCGGCAACTTCTAG

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002198 Short-chain dehydrogenase/reductase SDR (Family) PR00080 SDRFAMILY PF00106 adh_short PIRSF000126 11-beta-HSD1 IPR002347 Glucose/ribitol dehydrogenase (Family) PR00081 GDHRDH IPR016040 NAD(P)-binding domain (Domain) G3DSA:3.40.50.720 NAD(P)-bd IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site) PS00061 ADH_SHORT
SignalP	Bacteria, Gram-positive
TMHMM	No significant hit

Gilvo_00200 Gilvo_00220