Enter_00050 : CDS information

Location

Organism	Streptomyces maritimus
Strain
Entry name	Enterocin
Contig	AF254925
Start / Stop / Direction	4,934 / 5,710 / + [in whole cluster] 4,934 / 5,710 / + [in contig]
Location	4934..5710 [in whole cluster] 4934..5710 [in contig]
Type	CDS
Length	777 bp (258 aa)

Annotation

Category	2.2 modification addition of starter unit
Product	putative cinnamoyl-CoA hydratase
Product (GenBank)	putative enoyl-CoA hydratase EncI
Gene
Gene (GenBank)	encI
EC number	4.2.1.-
Keyword
Note
Note (GenBank)
Reference	ACC Q9KHL0 PmId [11137817] Cloning, sequencing and analysis of the enterocin biosynthesis gene cluster from the marine isolate 'Streptomyces maritimus': evidence for the derailment of an aromatic polyketide synthase. (Chem Biol. , 2000) [12511484] Characterization of benzoyl coenzyme A biosynthesis genes in the enterocin-producing bacterium "Streptomyces maritimus". (J Bacteriol. , 2003) comment [PMID: 11137817](2000) enterocin と wailupemycinsの生合成をコードするgene clusterの同定。 EncI: Enoyl-CoA hydratase 配列解析のみ。 PKS starter unitであるbenzoic acidの生合成経路(Figure 3)で、3番目の反応として cinnamoyl-CoA→3-hydroxy-3-phenylpropionyl-CoAを触媒すると考えられている。 --- 同じ著者らの続報。 [PMID: 12511484](2003) benzoyl-CoA生合成遺伝子についての報告。 EncI: Cinnamoyl-CoA hydratase encH, encI, encJの破壊mutantは、benzoyl-CoA-primed enterocinの収量がwildより下がった。 encI mutantは、cinnamic acid and enterocin産生が20-25%まで低下するが、d5-benzoic acidの補充でenterocin産生はwildレベルまで回復する。完全に抑制されないのは、一次代謝酵素が代替しているのかもしれない。 EncI cinnamoyl-CoA hydrataseはfatty acid enoyl-CoA hydratasesにAA配列類似性があり、Gly-116, Glu-119, and Glu-139は相同なfatty-acid-type hydratasesの触媒活性に必要な残基である。
Related Reference	ACC P52046 PmId [8655474] Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. (J Bacteriol. , 1996) comment Blast 51st, id33%, 9e-21 Clostridium acetobutylicum_crt 3-hydroxybutyryl-CoA dehydratase(EC 4.2.1.55) BCS (butyryl-CoA synthesis) operon (crt-bcd-etfB-etfA-hbd)のクローニングとシークエンス。 crt geneの上流にあるプロモーターによって制御されるtranscriptional unitであることを確認。 BCS operonを E. coliで大量発現して、crotonase, beta-hydroxybutyryl-CoA dehydrogenase activityを確認。 BCS operonを C. acetobutylicumで発現すると、crotonase, beta-hydroxybutyryl-CoA dehydrogenase, butyryl-CoA dehydrogenase activityが上昇。

Sequence

>putative cinnamoyl-CoA hydratase [putative enoyl-CoA hydratase EncI]
MSDVQQLLSSADLTVTVDSDVAVIAMSRPAKLNALTRGMRRDLAELLRHYGDGSQARGVV
ITGQAKAFSAGMDLREVAGPEVDLLAEMELFNDITRAALITEVPVVAALNGIAVGGAGEM
ALSFDARIATAQAAISWPETGVGLTVSNAASLFLPRLAGAPRALHLIMNSARLSASQALA
LGLLDDIVEPDDLIPAAIRLIHQWTPPGSSTAAHLRLMRPSLRAVEAAMAREGAAVRQVQ
ESGIAQAGIATVLAGHEK

>putative cinnamoyl-CoA hydratase [putative enoyl-CoA hydratase EncI]
ATGAGCGACGTACAGCAGTTGCTGAGCTCCGCCGACCTGACCGTCACGGTCGACAGCGAT
GTTGCTGTGATCGCGATGAGCCGTCCGGCGAAACTCAACGCCCTGACCCGCGGCATGCGC
CGGGACCTGGCCGAGCTGCTGCGCCACTACGGCGACGGCAGCCAGGCTCGCGGAGTGGTG
ATCACCGGCCAGGCAAAAGCCTTCTCTGCCGGAATGGACCTGCGCGAAGTCGCCGGCCCG
GAAGTCGACTTGCTGGCGGAGATGGAGCTGTTCAACGACATCACCCGCGCCGCTCTCATC
ACGGAAGTCCCGGTCGTGGCCGCCCTCAACGGCATCGCCGTCGGCGGCGCCGGTGAGATG
GCCCTGAGCTTCGACGCCCGCATCGCCACCGCTCAGGCCGCGATCTCATGGCCGGAGACA
GGTGTCGGGCTCACCGTGTCCAATGCCGCGAGCCTGTTCCTGCCCCGCCTCGCTGGCGCA
CCCCGAGCCCTGCACCTGATCATGAACAGTGCCCGGCTCAGCGCCTCCCAGGCACTGGCC
CTCGGTCTCCTCGACGACATCGTCGAGCCGGACGATCTCATCCCCGCCGCCATCCGGCTG
ATCCACCAGTGGACGCCGCCGGGTTCGTCCACGGCCGCACATCTGAGGCTGATGCGGCCC
TCGCTGCGTGCGGTAGAAGCGGCCATGGCACGCGAGGGCGCGGCCGTACGGCAGGTCCAG
GAGAGCGGCATTGCCCAGGCCGGCATCGCCACGGTGCTGGCTGGCCACGAAAAGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR001753 Crotonase, core (Domain) PF00378 ECH
SignalP	No significant hit
TMHMM	No significant hit

Enter_00040 Enter_00060