Urd_00130 : CDS information

Location

Organism	Streptomyces fradiae
Strain	Tü2717
Entry name	Urdamycin
Contig	X87093
Start / Stop / Direction	14,913 / 16,139 / + [in whole cluster] 3,123 / 4,349 / + [in contig]
Location	14913..16139 [in whole cluster] 3123..4349 [in contig]
Type	CDS
Length	1,227 bp (408 aa)

Annotation

Category	1.1 PKS
Product	polyketide synthase chain length factor subunit
Product (GenBank)	putative chain length determinant
Gene	urdB
Gene (GenBank)	UrdB
EC number
Keyword	type II PKS
Note
Note (GenBank)	gtg start
Reference	ACC Q54174 PmId [7592377] Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tu2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation. (J Bacteriol. , 1995) [14734170] Expression of the landomycin biosynthetic gene cluster in a PKS mutant of Streptomyces fradiae is dependent on the coexpression of a putative transcriptional activator gene. (FEMS Microbiol Lett. , 2004) comment [PMID: 7592377](1995) urdEFABCDを含むcosmid purd8をS.lividans TK24へ導入すると、urdamycin抵抗性を獲得。 urdamycin産生株S.fradiae Tu2717染色体から、urdE-C末+urdFABを含むfragment g領域を削除するとurdamycin産生不可。 AA配列解析で、UrdBは芳香族PKS gene clustersのCLFに似ている。 UrdAとUrdBはid36%で似ているが、UrdBはactive site cysteineを持っていない。 TcmK ketoacyl synthase or TcmL chain length factorのどちらかを障害してtetracenomycin C非産生にしたStreptomyces glaucescensのmutantで、urdFABを異種性発現したらどちらのmutantも相補され、tetracenomycin Cを産生できた。 -- [PMID: 14734170](2004) landomycin生合成clusterに関する論文だが、ホスト株としてStreptomyces fradiae Tu2717のPKS破壊mutant株(urdB and Cが不完全。urdamycin Aを産生できない。)を使用している。

PKS/NRPS Module

CLF	7..365

Sequence

>polyketide synthase chain length factor subunit [putative chain length determinant]
MNTGAVEVAVTGLGVVAPNGLGTDAYWAATRKGTSGIARISRFDPSRYPVQLAGEIEGFD
AKGHLPGRLLPQTDRMTQLALVAADWAFEDAAVRPGDLPEFEMGVITASSSGGFEFGQRE
LQALWSRGSRYVSAYQSFAWFYAVNSGQISIRNGMRGPSGVVVSDQAGGLDAVAQARRQI
RKGTRLVMSGAVDASICPWGWVAQMASNRLSTSRDPERAYLPFDDAAGGHVPGEGGALLV
LEELEQARARGARQIYGVIAGYGSTLDPRPGSGRPAGLRKAIELALADAGAAPEEIDVVF
ADAAAVPELDRIEAEALTQVFGARAVPVTAPKTMTGRLYSGAAPLDLAAAFLAMRDGVVP
PSVGVSPSPDHDLDLVVHQERAMTVRSALVIARGHGGFNSAVVVRAVG

>polyketide synthase chain length factor subunit [putative chain length determinant]
GTGAACACAGGCGCTGTCGAAGTCGCAGTCACCGGACTGGGCGTGGTCGCTCCCAACGGC
CTCGGCACGGACGCCTACTGGGCGGCGACCCGCAAGGGAACCAGCGGCATCGCACGGATC
TCCCGCTTCGACCCCTCGCGGTACCCGGTCCAACTGGCCGGTGAGATCGAGGGGTTCGAC
GCGAAGGGGCATCTGCCGGGCCGATTGCTGCCGCAGACCGACCGGATGACCCAGCTCGCT
CTCGTGGCCGCGGACTGGGCGTTCGAGGATGCCGCCGTGCGCCCCGGGGACCTGCCCGAG
TTCGAGATGGGTGTGATCACGGCCAGCTCCTCGGGCGGCTTCGAGTTCGGTCAGCGGGAG
CTGCAGGCCCTGTGGAGCCGGGGCAGCAGGTATGTCAGCGCGTACCAGTCCTTCGCCTGG
TTCTACGCCGTCAACAGCGGCCAGATCTCGATCCGCAACGGCATGCGTGGCCCGAGCGGC
GTGGTCGTCAGCGACCAGGCCGGCGGGCTCGACGCCGTGGCCCAGGCGCGACGGCAGATC
CGCAAGGGCACCAGGCTGGTGATGTCGGGCGCCGTCGACGCCTCGATCTGCCCCTGGGGC
TGGGTCGCGCAGATGGCAAGCAACCGGCTGAGCACCAGCCGCGACCCCGAGCGGGCCTAC
CTCCCCTTCGACGACGCCGCGGGTGGACACGTACCCGGCGAGGGCGGTGCCCTGCTCGTC
CTGGAGGAACTGGAGCAGGCCCGTGCTCGGGGCGCCCGGCAGATCTACGGCGTGATCGCC
GGGTACGGCTCCACGCTCGACCCCCGCCCCGGCAGCGGACGTCCCGCAGGCTTGCGCAAG
GCGATCGAGCTGGCGCTGGCCGATGCCGGGGCCGCACCCGAGGAGATCGATGTGGTGTTC
GCCGATGCGGCCGCGGTCCCCGAGCTGGACCGGATCGAGGCCGAGGCCCTCACCCAGGTG
TTCGGAGCCCGTGCAGTGCCGGTGACGGCACCTAAGACGATGACCGGCCGGCTCTACTCC
GGCGCCGCGCCCCTGGACCTGGCGGCCGCGTTCCTCGCCATGCGGGACGGAGTCGTCCCG
CCGTCCGTCGGCGTCTCTCCCTCTCCCGACCACGACCTCGACCTGGTCGTCCACCAGGAG
AGGGCCATGACAGTGCGCTCCGCCCTGGTGATCGCCCGCGGCCACGGCGGTTTCAACTCC
GCGGTCGTCGTGCGCGCCGTCGGATAG

CLF	7..365

CLF	19..1095

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR014030 Beta-ketoacyl synthase, N-terminal (Domain) PF00109 ketoacyl-synt IPR014031 Beta-ketoacyl synthase, C-terminal (Domain) PF02801 Ketoacyl-synt_C IPR016038 Thiolase-like, subgroup (Domain) G3DSA:3.40.47.10 Thiolase-like_subgr IPR016039 Thiolase-like (Domain) SSF53901 Thiolase-like
SignalP	No significant hit
TMHMM	No significant hit

Urd_00120 Urd_00140