Urd_00140 Urd_00140   Urd_00160 Urd_00160

Urd_00150 : CDS information

close this sectionLocation

Organism
StrainTü2717
Entry nameUrdamycin
Contig
Start / Stop / Direction16,689 / 17,474 / + [in whole cluster]
4,899 / 5,684 / + [in contig]
Location16689..17474 [in whole cluster]
4899..5684 [in contig]
TypeCDS
Length786 bp (261 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative polyketide C-9 ketoreductase
Product (GenBank)putative ketoreductase
GeneurdD
Gene (GenBank)UrdD
EC number
Keyword
Note
Note (GenBank)
Reference
ACC
PmId
[7592377] Cloning and characterization of a polyketide synthase gene from Streptomyces fradiae Tu2717, which carries the genes for biosynthesis of the angucycline antibiotic urdamycin A and a gene probably involved in its oxygenation. (J Bacteriol. , 1995)
comment
urdEFABCDを含むcosmid purd8をS.lividans TK24へ導入すると、urdamycin抵抗性を獲得。

AA配列解析で、urdDは芳香族polyketide gene clusterのKRsに高い類似性あり。
それらは新生polyketide鎖のC-9 keto基の還元に関与している。
Related Reference
ACC
Q56166
NITE
Jado_00110
PmId
[7881555] Cloning and characterization of polyketide synthase genes for jadomycin B biosynthesis in Streptomyces venezuelae ISP5230. (Microbiology. , 1994)
[9224566] Iterative type II polyketide synthases, cyclases and ketoreductases exhibit context-dependent behavior in the biosynthesis of linear and angular decapolyketides. (Chem Biol. , 1997)
comment
Blast 5th, id84%, 1e-123
Streptomyces venezuelae_ORF5(jadE)
Ketoreductase

--
[PMID:7881555](1994)
ORF5(=jadEに相当)は配列解析からketoreductaseであると提唱されている。
ORF2-ORF4領域(ORF5も含まれる)を含んだplasmidの染色体DNAへの組み込みは、jadomycin B産生を激しく抑制したが、成長や胞子色素沈着には影響なし。

--
[PMID: 9224566](1997)
minimal PKS + KR + CYCをjadomycin, daunorubicin, tetracenomycinのgenesで構成し、
そのplasmid発現株での産物を解析。
JadEはdecaketideだけでなく、octaketideも還元できる。

close this sectionSequence

selected fasta
>putative polyketide C-9 ketoreductase [putative ketoreductase]
MTQQNPKVAIVTGATSGIGLAVTRLLGRQGHRVFLCARTEDSVTSTVKQLLDEGLEVDGA
PCDVRSADDVERFVQRAVHRFGTIDVLVNNAGRSGGGVTADIADELWHDVIDTNLNSVFR
MTREVLNTGGMRHKDRGRIINIASTAGKQGVVLGAPYSASKHGVVGFTKALGNELAPTGI
TVNAVCPGYVETPMAQRVRAGYAAAYDTSEDAILEKFQAKIPLGRYSTPEEVAGLVGYLA
SDTAASITSQALNVCGGLGNF
selected fasta
>putative polyketide C-9 ketoreductase [putative ketoreductase]
ATGACGCAGCAAAACCCGAAGGTCGCCATCGTCACGGGCGCGACCAGCGGCATCGGCCTG
GCGGTGACCAGGCTGCTGGGCCGCCAGGGGCACCGGGTGTTCCTGTGCGCCCGTACCGAG
GACAGCGTGACCAGTACGGTCAAGCAGCTCCTCGACGAGGGACTGGAGGTGGACGGCGCA
CCCTGCGACGTCCGTTCCGCGGACGACGTGGAGCGCTTCGTGCAGCGGGCAGTCCACCGC
TTCGGCACCATCGACGTGCTGGTCAACAATGCCGGTCGATCCGGCGGCGGCGTCACCGCC
GACATCGCCGACGAACTCTGGCACGACGTCATCGACACCAACCTCAACAGCGTCTTCCGG
ATGACCCGCGAGGTGCTCAACACCGGTGGGATGCGGCACAAGGACCGGGGGCGCATCATC
AACATCGCCTCCACCGCCGGCAAGCAGGGCGTCGTCCTCGGCGCCCCCTACTCCGCCTCC
AAACACGGCGTCGTCGGCTTCACCAAGGCCCTCGGCAACGAGCTCGCCCCGACCGGCATC
ACCGTCAACGCGGTCTGCCCCGGTTATGTCGAGACCCCCATGGCCCAGCGCGTGCGTGCC
GGGTACGCCGCCGCGTACGACACCTCCGAGGACGCGATCCTGGAGAAGTTCCAGGCGAAG
ATCCCCCTCGGTCGCTACTCGACCCCCGAGGAGGTCGCGGGTCTCGTCGGCTACCTCGCC
TCCGACACCGCCGCCTCCATCACCTCGCAGGCGCTGAACGTCTGCGGTGGGCTGGGCAAC
TTCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[82-93] 5.29999657550189e-15 PR00080 [137-145] 5.29999657550189e-15 PR00080 [157-176] 5.29999657550189e-15 PR00080
PR00080   SDRFAMILY
[7-175] 3.7e-36 PF00106
PF00106   adh_short
[1-253] 4.39999967439747e-16 PIRSF000126
PIRSF000126   11-beta-HSD1
IPR002347 Glucose/ribitol dehydrogenase (Family)
[8-25] 6.29998179434732e-50 PR00081 [82-93] 6.29998179434732e-50 PR00081 [131-147] 6.29998179434732e-50 PR00081 [157-176] 6.29998179434732e-50 PR00081 [178-195] 6.29998179434732e-50 PR00081 [222-242] 6.29998179434732e-50 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[4-258] 1.39999999999997e-83 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
IPR020904 Short-chain dehydrogenase/reductase, conserved site (Conserved_site)
[144-172] PS00061
PS00061   ADH_SHORT
SignalP No significant hit
TMHMM No significant hit
Urd_00140 Urd_00140   Urd_00160 Urd_00160
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