Urd_00210 Urd_00210   Urd_00230 Urd_00230

Urd_00220 : CDS information

close this sectionLocation

Organism
StrainTü2717
Entry nameUrdamycin
Contig
Start / Stop / Direction24,522 / 25,508 / + [in whole cluster]
7,032 / 8,018 / + [in contig]
Location24522..25508 [in whole cluster]
7032..8018 [in contig]
TypeCDS
Length987 bp (328 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category2.3 modification addition of sugar moiety
Productputative dTDP-glucose 4,6-dehydratase
Product (GenBank)dNDP-glucose 4,6-dehydratase
Gene
Gene (GenBank)urdH
EC number4.2.1.46
Keyword
  • D-olivose
  • L-rhodinose
Note
Note (GenBank)
  • UrdH
Reference
ACC
PmId
[10658661] Two new tailoring enzymes, a glycosyltransferase and an oxygenase, involved in biosynthesis of the angucycline antibiotic urdamycin A in Streptomyces fradiae Tu2717. (Microbiology. , 2000)
comment
urdHは、8.05kbp fragmentから同定された7genesのうちのひとつ。
urdG, urdH and urdZ1はdeoxysugar生合成genesに似ている。

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参照)[PMID: 11094336]
urdamycin deoxysugar(NDP-D-plivose, NDP-L-rhodinose)生合成経路のFigあり。
UrdH = NDP-D-glucose → NDP-4-keto-6-deoxy-D-Glucose
Related Reference
ACC
Q56173
NITE
Grana_00110
PmId
[7476861] Identification of Streptomyces violaceoruber Tu22 genes involved in the biosynthesis of granaticin. (Mol Gen Genet. , 1995)
[9831526] The granaticin biosynthetic gene cluster of Streptomyces violaceoruber Tu22: sequence analysis and expression in a heterologous host. (Chem Biol. , 1998)
comment
Blast 15th, id66%, 1e-120
Streptomyces violaceoruber_graE(gra-orf17)
dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)

--
[PMID: 7476861](1995) abstract
ORF2はstrEに似ている。
E.coliでORF2を発現させると、dTDP-glucose→dTDP-4-keto-6-deoxy-D-glucoseへの変換を触媒することができた(controlの5倍)。
よって、ORF2はdTDP-glucose dehydrataseをコードする。

--
[PMID: 9831526](1998)
gra cluster genesのまとめ。renameあり。
ORF2→ORF17
ORF3→ORF16

close this sectionSequence

selected fasta
>putative dTDP-glucose 4,6-dehydratase [dNDP-glucose 4,6-dehydratase]
MNILVTGAAGFIGSHFVRSLLADTYSGWEGARVTALDKLTYAGNRNNLPPSNPRLEFVRG
DVCDRALLRELLPGHHAVVHFAAESHVDRSLEGAGEFFRTNVLGTQTLLDAVLDSGVERV
VHVSTDEVYGSIEQGSWTEDWPLQPNSPYAASKACSDLVARAYCAPTEVDLSITRCSNNY
GPHQHPEKVIPRFVTNLLEGRQVPLYGDGRNVREWLHVEDHCRGIHLVLNKGQAGEIYNI
GGGNEYTNLALTEKLLELTGAGPEMIRRVPDRKAHDLRYSIDESKIREKLGYAPRISFEQ
GLSDTVAWYRDNPDWWKSIEHGGDSAAA
selected fasta
>putative dTDP-glucose 4,6-dehydratase [dNDP-glucose 4,6-dehydratase]
ATGAACATCCTGGTCACCGGCGCGGCCGGTTTCATCGGCTCGCACTTCGTCCGCAGCCTG
CTGGCCGACACCTACTCCGGCTGGGAGGGAGCCCGGGTCACTGCCCTGGACAAACTGACC
TACGCGGGCAACCGGAACAACCTGCCGCCCTCAAACCCGCGCCTGGAGTTCGTGCGGGGC
GACGTGTGCGACCGCGCCCTGCTCCGCGAGCTGCTGCCCGGGCACCACGCCGTGGTCCAT
TTCGCGGCGGAATCCCACGTGGACCGCTCCCTCGAAGGCGCGGGCGAGTTCTTCCGCACG
AACGTCCTCGGTACGCAGACACTCCTCGACGCCGTACTGGACAGCGGAGTCGAGCGGGTC
GTTCACGTCTCCACCGACGAGGTGTACGGCTCGATCGAGCAAGGCTCCTGGACCGAGGAC
TGGCCGCTGCAGCCCAATTCTCCGTACGCCGCCTCGAAGGCATGCTCGGACCTGGTCGCG
CGAGCCTACTGCGCACCCACGGAGGTGGACCTCTCCATCACCCGCTGCTCCAACAACTAC
GGCCCGCACCAGCACCCTGAGAAGGTCATTCCCAGGTTCGTCACGAACCTCCTCGAAGGA
CGCCAGGTCCCGCTGTACGGCGACGGCCGCAATGTGCGCGAGTGGCTCCACGTGGAGGAC
CACTGCCGCGGTATCCACCTGGTGCTCAACAAGGGGCAGGCCGGCGAGATCTACAACATC
GGCGGGGGCAACGAGTACACCAACCTCGCCCTCACCGAGAAACTGCTCGAACTGACGGGT
GCCGGCCCGGAGATGATCCGCCGCGTCCCCGACCGCAAGGCGCACGACCTGCGGTACTCG
ATCGACGAGTCCAAGATCCGCGAGAAGCTCGGCTACGCCCCGCGGATCAGCTTCGAACAG
GGCCTGTCCGACACCGTCGCCTGGTACCGCGACAACCCGGACTGGTGGAAGAGCATCGAG
CATGGCGGGGACAGTGCGGCGGCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR001509 NAD-dependent epimerase/dehydratase (Domain)
[3-241] 9.9e-70 PF01370
PF01370   Epimerase
IPR005888 dTDP-glucose 4,6-dehydratase (Family)
[2-319] 5.20000000000001e-122 TIGR01181
TIGR01181   DTDP_gluc_dehyt
IPR016040 NAD(P)-binding domain (Domain)
[1-188] 3.19999999999996e-65 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP
[1-22] 0.893 Signal
Bacteria, Gram-negative   
[1-22] 0.772 Signal
Eukaryota   
[1-22] 0.221 Signal
Bacteria, Gram-positive   
TMHMM No significant hit
Urd_00210 Urd_00210   Urd_00230 Urd_00230
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