Acla_00220 Acla_00220   Acla_00240 Acla_00240

Acla_00230 : CDS information

close this sectionLocation

Organism
StrainATCC 31615
Entry nameAclacinomycin A
Contig
Start / Stop / Direction26,601 / 25,798 / - [in whole cluster]
10,446 / 9,643 / - [in contig]
Locationcomplement(25798..26601) [in whole cluster]
complement(9643..10446) [in contig]
TypeCDS
Length804 bp (267 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.3 modification reduction
Productputative aklaviketone C-7 ketoreductase
Product (GenBank)putative aklaviketone reductase
Gene
Gene (GenBank)aknU
EC number
Keyword
Note
Note (GenBank)
  • AknU
Reference
ACC
PmId
[11016846] A gene cluster from Streptomyces galilaeus involved in glycosylation of aclarubicin. (Mol Gen Genet. , 2000)
comment
anthracycline生合成gene clusterの15kb領域をクローニング、特徴づけ。

AknU: aklaviketone reductase (KRII)

Streptomyces sp. C5のaklaviketone reductase (DauE)に最も強い類似あり。

glycosyltransferase (aknS), aminomethylase (aknX), glucose-1-phosphate thymidylyltransferase (aknY) とunidentified glycosylation functions (aknT and aknV)を含むplasmid pSgs4は、aklavinoneを蓄積するS. galilaeus mutants H063と、rhodinose and deoxyfucose residuesを持つaklavinoneを産生するH054でaclacinomycinsの産生を回復。(aknUもpSgs4に含まれている。)

また、ε-rhodomycinone(RHO)を蓄積するS. peucetius mutants M18において、
pSgs4の導入はL-rhamnosyl-RHO と L-daunosaminyl-RHO を、
pSgs42(aknS, aknT, aknU, aknV)の導入はL-rhamnosyl-RHO のみを産生する。
Related Reference
ACC
Q53882
NITE
Dauno_00060
PmId
[8655529] Cloning, sequencing, and analysis of aklaviketone reductase from Streptomyces sp. strain C5. (J Bacteriol. , 1996)
comment
Blast 4th, id66%, 2e-74
Streptomyces sp. (strain C5)_dauE
Aklaviketone reductase

recombinant DauE を含んだStreptomyces lividans TK24 の抽出液は、
aklaviketone → aklavinone
maggiemycin → epsilon-rhodomycinone(RHO)
7-oxodaunomycinone → daunomycinone
へのNADPH-specificな変換を触媒した。
これらの変換はすべてposition 7でのketo基の還元。

close this sectionSequence

selected fasta
>putative aklaviketone C-7 ketoreductase [putative aklaviketone reductase]
MNRAARPTVRGMSAIAEPTAPRGVIVTGGGTGIGRATAHAFADRGDRVLVVGRTAATLAG
TAEGHPGISVLTADLTDPDGPRAITDAALDALGRIDVLVNNAATGGFAGLAETEPEAARE
QFDSNLLAPLLLTRQTLDALSADGGGTVLNIGSAGALGRRAWPQNGVYGAAKAGLDFLTR
TWAVELAPRGIRVLGLAPGVIDTGIGERSGMSREAYAGFLGQIAARVPAGRVGRPEDIAW
WAVQLADPRAAYATGAVLAVDGGLSLT
selected fasta
>putative aklaviketone C-7 ketoreductase [putative aklaviketone reductase]
GTGAATCGAGCGGCCCGGCCTACGGTCCGGGGCATGAGCGCCATAGCAGAACCGACAGCG
CCGCGGGGCGTGATCGTCACCGGCGGCGGGACCGGGATCGGGCGGGCCACGGCCCACGCC
TTCGCCGACCGCGGCGACCGGGTACTCGTCGTAGGCCGCACCGCCGCGACCCTGGCCGGG
ACCGCCGAGGGCCACCCCGGCATCAGTGTCCTGACCGCCGATCTCACCGATCCGGACGGT
CCGCGCGCCATCACCGACGCGGCGCTGGACGCGTTGGGCCGTATCGACGTCCTCGTGAAC
AACGCGGCCACCGGTGGGTTCGCCGGCCTGGCGGAGACCGAGCCGGAGGCCGCCCGCGAG
CAGTTCGACAGCAACCTCCTGGCCCCGCTGCTGCTCACCCGGCAGACCCTGGACGCCCTG
TCGGCCGACGGCGGGGGAACCGTGCTGAACATCGGCTCGGCCGGAGCTCTGGGCCGCCGG
GCCTGGCCGCAGAACGGTGTCTACGGCGCCGCCAAGGCCGGGCTCGACTTCCTCACCCGG
ACCTGGGCCGTCGAACTGGCACCCCGCGGCATCCGTGTCCTCGGCCTGGCGCCCGGCGTG
ATCGACACCGGCATCGGTGAACGCTCGGGCATGTCACGGGAGGCCTACGCGGGCTTCCTC
GGGCAGATCGCCGCCCGCGTCCCGGCCGGCCGGGTCGGCCGGCCCGAGGACATCGCCTGG
TGGGCCGTCCAGCTGGCCGACCCGCGCGCCGCTTACGCGACCGGAGCGGTGCTCGCGGTC
GACGGCGGTCTGTCCCTCACCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR002198 Short-chain dehydrogenase/reductase SDR (Family)
[93-104] 1.09999909120787e-09 PR00080 [146-154] 1.09999909120787e-09 PR00080 [168-187] 1.09999909120787e-09 PR00080
PR00080   SDRFAMILY
[24-186] 7.7e-25 PF00106
PF00106   adh_short
IPR002347 Glucose/ribitol dehydrogenase (Family)
[23-40] 6.29998179434732e-34 PR00081 [93-104] 6.29998179434732e-34 PR00081 [140-156] 6.29998179434732e-34 PR00081 [168-187] 6.29998179434732e-34 PR00081 [189-206] 6.29998179434732e-34 PR00081 [228-248] 6.29998179434732e-34 PR00081
PR00081   GDHRDH
IPR016040 NAD(P)-binding domain (Domain)
[22-266] 1.29999999999998e-69 G3DSA:3.40.50.720
G3DSA:3.40.50.720   NAD(P)-bd
SignalP No significant hit
TMHMM No significant hit
Acla_00220 Acla_00220   Acla_00240 Acla_00240
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