Alip_00040 Alip_00040   Alip_00060 Alip_00060

Alip_00050 : CDS information

close this sectionLocation

Organism
StrainTü117
Entry nameAlpha-lipomycin
Contig
Start / Stop / Direction5,947 / 5,477 / - [in whole cluster]
5,947 / 5,477 / - [in contig]
Locationcomplement(5477..5947) [in whole cluster]
complement(5477..5947) [in contig]
TypeCDS
Length471 bp (156 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category4.1 transcriptional regulator
ProductMarR family transcriptional repressor
Product (GenBank)LipReg3
Gene
Gene (GenBank)lipReg3
EC number
Keyword
Note
Note (GenBank)
  • putative MarR-family transcriptional regulator
Reference
ACC
PmId
[16723573] Biosynthetic gene cluster for the polyenoyltetramic acid alpha-lipomycin. (Antimicrob Agents Chemother. , 2006)
[19585113] Characterization and analysis of the regulatory network involved in control of lipomycin biosynthesis in Streptomyces aureofaciens Tu117. (Appl Microbiol Biotechnol. , 2010)
comment
[PMID: 16723573](2006)
alpha-lipomycin生合成遺伝子クラスターに関する文献。

lipReg3(156 aa): regulation

lipReg3 自体の機能実験は行われておらず、MarR-family transcriptional regulatorとの相同性が示されているのみ。

---
[PMID: 19585113](2010)
alpha-lipomycin生合成に関与するregulatory networkに関する文献。

lipReg3挿入変異株では野生株に比べてalpha-lipomycinの生産が4倍以上に上昇し、この変異株にlipReg3を相補するとalpha-lipomycinの生産が野生株の1/3程度となることが示されており、LipReg3はalpha-lipomycin生産に対してnegativeに制御している可能性が示唆されている。

さらに、lipReg3挿入変異株ではlipEx1（lipomycin exporterとして機能することが推測）の発現量が野生株に比べて有意に上昇しており、LipReg3はlipEx1の発現を制御している可能性が示されている。
Family/Domain
FD
IPR000835
comment
[IPR000835] HTH transcriptional regulator, MarR (Family)

function:
GO:0003700 sequence-specific DNA binding transcription factor activity

close this sectionSequence

selected fasta
>MarR family transcriptional repressor [LipReg3]
MEVSVARDPEVTASELVAALTETVSPLLRHAGTVFANNQVPFSQASLLTDLYERGAPQRM
STLAQTFDVAPRTVTALVDGLERRGLVQRAPDPDDRRAVLVGVTDKGNALMQEIEWGRQA
LADELVNGLTRDERVEFARLLNKLRPAPKSEKGSQA
selected fasta
>MarR family transcriptional repressor [LipReg3]
ATGGAGGTGTCCGTGGCTCGAGATCCTGAGGTCACGGCTTCCGAGCTCGTTGCGGCTCTG
ACGGAGACGGTGTCTCCGCTTCTGCGCCACGCCGGGACCGTGTTCGCCAACAATCAGGTC
CCCTTCTCCCAGGCCAGCCTGCTGACCGACCTGTACGAGCGGGGAGCCCCCCAACGCATG
AGCACGCTGGCCCAGACGTTCGACGTGGCCCCGCGCACCGTCACCGCCCTCGTCGACGGA
CTCGAGCGCCGCGGCCTCGTCCAGCGGGCCCCGGACCCGGATGATCGTCGCGCCGTTCTG
GTCGGCGTCACCGACAAGGGCAACGCGCTGATGCAGGAGATCGAGTGGGGCAGGCAGGCC
CTCGCCGACGAACTCGTCAACGGTCTGACCCGCGACGAACGCGTGGAGTTCGCGCGCCTG
CTCAACAAGCTCCGCCCGGCGCCCAAGAGCGAGAAGGGGTCCCAGGCATGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR000835 Transcription regulator HTH, MarR-type (Family)
[13-146] PS50995
PS50995   HTH_MARR_2
[33-134] 7.99999145876753e-20 SM00347
SM00347   HTH_MARR
[43-99] 7.59999999999999e-13 PF01047
PF01047   MarR
IPR011991 Winged helix-turn-helix transcription repressor DNA-binding (Domain)
[8-145] 7.7e-32 G3DSA:1.10.10.10
G3DSA:1.10.10.10   Wing_hlx_DNA_bd
SignalP
[1-36] 0.246 Signal
Bacteria, Gram-positive   
[1-36] 0.191 Signal
Bacteria, Gram-negative   
TMHMM No significant hit
Alip_00040 Alip_00040   Alip_00060 Alip_00060
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