Alip_00080 : CDS information

Location

Organism	Streptomyces aureofaciens
Strain	Tü117
Entry name	Alpha-lipomycin
Contig	DQ176871
Start / Stop / Direction	11,284 / 10,415 / - [in whole cluster] 11,284 / 10,415 / - [in contig]
Location	complement(10415..11284) [in whole cluster] complement(10415..11284) [in contig]
Type	CDS
Length	870 bp (289 aa)

Annotation

Category	2.3 modification addition of sugar moiety
Product	putative glucose-1-phosphate thymidylyltransferase putative dTDP-glucose synthase
Product (GenBank)	LipDig1
Gene
Gene (GenBank)	lipDig1
EC number	2.7.7.24
Keyword	D-digitoxose
Note
Note (GenBank)	putative dNDP-glucose synthase
Reference	ACC Q30CS7 PmId [16723573] Biosynthetic gene cluster for the polyenoyltetramic acid alpha-lipomycin. (Antimicrob Agents Chemother. , 2006) comment alpha-lipomycin生合成遺伝子クラスターに関する文献。 lipDig1(289 aa): NDP-D-glucose synthase lipDig1自体の機能実験は行われていないが相同性より、D-digitoxose moietyを生産する際のD-glucose-1-phosphateよりNDP-D-glucoseへと変換するNDP-D-glucose synthaseであると予想している。
Related Reference	ACC Q9AGY4 PmId [12147463] Homologs of the Rml enzymes from Salmonella enterica are responsible for dTDP-beta-L-rhamnose biosynthesis in the gram-positive thermophile Aneurinibacillus thermoaerophilus DSM 10155. (Appl Environ Microbiol. , 2002) comment <Glucose-1-phosphate thymidylyltransferase (rmlA), Aneurinibacillus thermoaerophilus, BLAST 26; id=58%> Glucose-1-phosphate thymidylyltransferase (rmlA)をE. coliで発現させ、活性等の諸性質について調べている。

Sequence

>putative glucose-1-phosphate thymidylyltransferase [LipDig1]
MRGIVLAGGSGTRLRPVTRVASKQLLPVYNKPMIYYPLSVLMHAGIREILIISSPGFLGS
YRDLLGDGRWLGLEISYAEQPSPDGLADALRIGRHFAGDDTIALILGDNIFYSHQLPAML
QGERQRLDGCTLFGYPVADPERYGIAVTDADGTLLSLEEKPEKPRSNLAVTGLYFYDNDA
VDLVRGLTPSARGELEITDLNALYLTAGRARLVNLGRGSAWFDTGTHDSLLDAATFVQLI
EKRQGIRIACIEEVAYRMGFIDADQLFEIGRRTGMSTEYGRYVIDLAGT

>putative glucose-1-phosphate thymidylyltransferase [LipDig1]
ATGCGTGGAATCGTCCTGGCAGGGGGATCCGGCACGCGGCTGAGGCCCGTCACCCGCGTC
GCCTCGAAGCAGCTCCTGCCGGTCTACAACAAGCCGATGATCTACTACCCCCTGTCCGTG
CTGATGCACGCGGGCATCCGGGAGATCCTGATCATCAGCTCGCCGGGGTTCCTCGGCTCC
TACCGCGATCTGCTCGGTGACGGCCGATGGCTGGGCCTGGAGATCTCCTACGCCGAGCAG
CCGAGCCCCGACGGCCTCGCCGACGCCCTGCGCATCGGCCGGCACTTCGCGGGGGACGAC
ACCATCGCCCTCATTCTCGGCGACAACATCTTCTACAGCCACCAGCTGCCCGCCATGCTC
CAGGGCGAGCGGCAACGGCTCGACGGCTGCACCCTGTTCGGCTACCCGGTGGCCGACCCC
GAGCGGTACGGCATCGCCGTCACGGACGCCGACGGAACGCTGCTCTCGCTGGAGGAGAAG
CCGGAGAAGCCCCGGTCGAACCTGGCGGTGACCGGCCTGTACTTCTACGACAACGACGCC
GTGGACCTGGTACGTGGACTGACCCCCTCGGCCCGCGGCGAGCTGGAGATCACCGATCTC
AACGCCCTGTATCTGACGGCCGGCCGGGCCCGCCTGGTCAATCTGGGCCGCGGCAGCGCC
TGGTTCGACACCGGCACGCACGACAGCCTGCTCGACGCGGCGACGTTCGTGCAGCTCATC
GAGAAGCGCCAGGGCATCCGCATCGCCTGCATCGAGGAGGTGGCGTACCGCATGGGCTTC
ATCGACGCCGACCAGCTGTTCGAGATCGGCCGGCGGACCGGCATGAGCACCGAGTACGGC
CGGTACGTGATCGATCTGGCCGGCACCTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR005835 Nucleotidyl transferase (Domain) PF00483 NTP_transferase IPR005907 Glucose-1-phosphate thymidylyltransferase, short form (Family) TIGR01207 RmlA
SignalP	No significant hit
TMHMM	No significant hit

Alip_00070 Alip_00090