Avil_00430 Avil_00430   Avil_00450 Avil_00450

Avil_00440 : CDS information

close this sectionLocation

Organism
StrainTü57
Entry nameAvilamycin
Contig
Start / Stop / Direction44,218 / 42,761 / - [in whole cluster]
44,218 / 42,761 / - [in contig]
Locationcomplement(42761..44218) [in whole cluster]
complement(42761..44218) [in contig]
TypeCDS
Length1,458 bp (485 aa)
Click on the icon to see Genetic map.

close this sectionAnnotation

Category3.4 other modification
Producthalogenase
Product (GenBank)
GeneaviH
Gene (GenBank)
EC number
Keyword
  • dichloroisoeverninic acid
Note
Note (GenBank)
Reference
PmId
[11410376] Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tu57 and production of new antibiotics. (Chem Biol. , 2001)
comment
avilamycin A生合成遺伝子クラスターの報告。

AviH: PltA from P.fluorescens Pf-5 [Proposed function:Modification (halogenation)]

orsellinic acidからdichloroisoeverninic acid unitを合成する修飾に関与すると推測している。
aviG4 knock out mutant、aviG4-aviH knock out double mutantを作製。検出された化合物の構造から、AviG4はmethyltransferase、AviHはhalogenaseであることが示された。

close this sectionSequence

selected fasta
>halogenase
MSKKVLVIGGGPAGSTAASLLSKAGMSVKLLERETFPRYHIGESIASSCRTIIDLVGALE
EVDSRGYTVKNGVLLRWGKEDWAIDWPKIFGPDVRSWQVDRDDFDHVLLKNAVKQGADVT
EGVTVKRVLFDGDRAAGAEWLDPDTGELVSEEFDYVIDASGRAGVISKHLKNRRPHEIFR
NVAIWGYWQGGSLLPSSPTGGINVISAPDGWYWVIPLRGDRYSVGFVCHQDRFLERRREH
ADLEAMLASLVEESPTVRDLMANGVYQPGVRVEQDFSYVADSFHGPGYYLAGDAACFLDP
LLSTGVHLALYSGMLAATSVLATVNKDVTEEEAGAFYESLYRNAYQRLFTLVSGVYQQQA
GKAAYFELADAMTPERATAEYEQVDGAVAFAELVAGLADIHDAVTGTHEDRAQQAHAQAD
ALPEDNSVRQLLAAAENARLMAAAGMPAAPVSEAPGKMDAHDLYDPATGLYLVTSPTLGI
GRSRP
selected fasta
>halogenase
ATGTCCAAAAAAGTGCTCGTGATCGGCGGTGGGCCGGCGGGATCCACGGCGGCCTCCTTG
CTCAGCAAGGCCGGCATGTCCGTGAAGCTATTGGAACGGGAGACCTTCCCGCGCTACCAC
ATCGGCGAGTCGATCGCCTCCTCGTGCCGCACCATCATCGACCTCGTCGGCGCGCTGGAG
GAGGTCGACTCCCGCGGTTACACGGTCAAGAACGGCGTGCTGCTGCGCTGGGGCAAGGAG
GACTGGGCGATCGACTGGCCGAAGATCTTCGGTCCCGACGTCCGCTCCTGGCAGGTCGAC
CGCGACGACTTCGACCACGTGCTCCTGAAGAACGCGGTCAAGCAGGGCGCCGACGTCACC
GAGGGCGTCACGGTCAAGCGCGTGCTGTTCGACGGCGACCGCGCGGCTGGGGCGGAGTGG
CTGGACCCCGACACCGGTGAGCTGGTCAGCGAGGAGTTCGACTACGTCATCGACGCCTCG
GGCCGGGCCGGCGTCATCTCCAAGCACCTGAAGAACCGCCGCCCGCACGAGATCTTCCGC
AACGTCGCGATCTGGGGCTACTGGCAGGGCGGCTCGCTGCTGCCCAGTTCGCCCACAGGG
GGCATCAACGTCATCAGCGCCCCGGACGGCTGGTACTGGGTGATCCCGCTGCGCGGGGAC
CGGTACAGCGTCGGTTTCGTCTGCCACCAGGACCGGTTCCTGGAGCGCCGCCGGGAACAC
GCCGACCTGGAGGCGATGCTGGCCTCGCTGGTCGAGGAATCCCCGACCGTGCGCGACCTC
ATGGCGAACGGCGTCTACCAGCCGGGCGTACGGGTGGAGCAGGACTTCTCCTACGTCGCC
GACAGCTTCCACGGCCCCGGCTACTACCTCGCCGGCGACGCCGCCTGCTTCCTCGACCCG
CTGCTGTCCACCGGCGTCCACCTGGCGCTCTACAGCGGCATGCTGGCCGCGACGTCCGTG
CTCGCCACGGTGAACAAGGACGTCACGGAGGAGGAGGCCGGGGCGTTCTACGAGTCGCTC
TACCGCAACGCCTACCAGCGGCTGTTCACCCTGGTGTCCGGCGTGTACCAGCAACAGGCC
GGCAAGGCCGCCTACTTCGAGCTGGCCGACGCGATGACCCCGGAGCGCGCCACGGCCGAG
TACGAGCAGGTCGACGGCGCGGTGGCCTTCGCCGAACTGGTCGCGGGACTGGCCGACATC
CACGACGCCGTCACCGGCACGCACGAGGACCGCGCCCAGCAGGCGCACGCCCAGGCCGAC
GCCCTGCCGGAGGACAACTCCGTCCGCCAGTTGCTCGCGGCGGCCGAGAACGCCCGTTTG
ATGGCGGCGGCCGGGATGCCCGCCGCACCGGTCAGCGAGGCTCCCGGCAAGATGGACGCC
CACGACCTCTACGACCCCGCCACGGGTCTCTACCTGGTGACCAGCCCGACGCTGGGAATC
GGCCGGTCCAGGCCCTGA

close this sectionFeature

BLASTP
Database:UniProtKB:2011_09 		show BLAST table
InterPro
Database:interpro:38.0
IPR003042 Aromatic-ring hydroxylase-like (Domain)
[4-26] 1.1e-09 PR00420 [152-167] 1.1e-09 PR00420 [285-300] 1.1e-09 PR00420
PR00420   RNGMNOXGNASE
IPR006905 Tryptophan halogenase (Family)
[4-81] 4.5e-12 PF04820 [96-365] 7.1e-22 PF04820
PF04820   Trp_halogenase
SignalP No significant hit
TMHMM No significant hit
Avil_00430 Avil_00430   Avil_00450 Avil_00450
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