Bena_00010 : CDS information

Location

Organism	Streptomyces sp.
Strain	A2991200
Entry name	Benastatin
Contig	AM501485
Start / Stop / Direction	951 / 1 / - [in whole cluster] 951 / 1 / - [in contig]
Location	complement(1..951) [in whole cluster] complement(1..951) [in contig]
Type	CDS
Length	951 bp (316 aa)

Annotation

Category	5.1 general function
Product	putative phosphotransferase
Product (GenBank)
Gene	benK
Gene (GenBank)
EC number
Keyword
Note	The N-terminal position was modified from original reference table.
Note (GenBank)
Reference	PmId [17439117] Molecular analysis of the benastatin biosynthetic pathway and genetic engineering of altered fatty acid-polyketide hybrids. (J Am Chem Soc. , 2007) comment 該当UniProt entryなし。 --- Benastatin A and B生合成をコードする全遺伝子座のクローニング、シークエンス。 flanking regionsの削除とS. albus, S. lividansでの異種性発現によってclusterが同定されている。 BenK(322aa): carbohydrate kinase FigureとTableのみ。本文での詳細記載なし。
Family/Domain	FD IPR011611, IPR002173 comment [IPR011611] PfkB (Domain) [IPR002173] Carbohydrate/puine kinase, PfkB, conserved site (Conserved_site)

Sequence

>putative phosphotransferase
MRIAITGSVATDHLMTFPGRIADQFLPDRLDRVSLSFLVDTLEVRDGGVAANIAYGLALL
GHRPLLIASVGTDFAEYGARLAALGVDLSAIRVSPTRHTARFLCTTDTDGNQLASFYPGA
MSEAPAIDLTAVLRQRGGADLVLVGADAPEAMARHTRTCRSMGVRFVADPSQQLARLDGP
QIRGLVAGADHLFTNEYERELLLAKTGWAPEDVLAQVSTWVTTRAEKGSRVEQTGRPPSE
VPAVPVATPVDPTGVGDAFRAGYLSALAAGGDPVRCARAGSALAALALTTVGTQTYTLAS
GGSDDLVTAPVQGTSM

>putative phosphotransferase
ATGCGCATCGCGATCACCGGTTCGGTCGCCACGGACCATCTCATGACCTTCCCCGGCCGC
ATCGCCGACCAGTTCCTGCCCGACCGCCTGGACCGTGTCTCCCTCTCCTTCCTCGTGGAC
ACTCTGGAGGTTCGGGACGGCGGAGTCGCGGCCAACATCGCCTACGGGCTCGCCCTGCTC
GGCCACCGACCGCTCCTGATCGCCTCGGTCGGGACGGACTTCGCGGAGTACGGTGCCCGG
CTGGCCGCCCTGGGAGTGGACCTCTCCGCCATCAGAGTCTCCCCCACCCGCCATACCGCC
CGCTTTCTGTGCACGACGGACACGGACGGCAACCAGCTCGCTTCCTTCTACCCGGGGGCG
ATGAGCGAGGCGCCCGCCATTGACCTCACCGCCGTACTGCGACAGCGCGGCGGCGCCGAT
CTCGTGCTCGTCGGTGCCGACGCCCCCGAGGCGATGGCGCGCCACACCAGGACCTGTCGC
TCCATGGGCGTGCGCTTCGTCGCCGACCCTTCCCAGCAACTGGCCCGGCTCGACGGCCCG
CAGATCCGGGGGCTCGTCGCCGGGGCGGACCACCTGTTCACGAACGAGTACGAACGCGAA
CTGCTCCTCGCCAAGACAGGCTGGGCCCCGGAGGACGTACTGGCGCAGGTCTCCACCTGG
GTGACCACCCGCGCCGAGAAGGGCAGCCGCGTCGAGCAGACCGGCAGGCCGCCGTCCGAG
GTCCCGGCCGTGCCCGTCGCGACGCCGGTCGACCCGACAGGGGTTGGTGACGCCTTCCGC
GCCGGCTATCTGTCGGCTCTCGCTGCGGGTGGTGATCCGGTTCGGTGCGCTCGGGCGGGC
TCGGCCCTCGCCGCCCTGGCCCTCACCACCGTCGGCACCCAGACGTACACGCTGGCCTCA
GGCGGATCGGACGACCTCGTCACAGCACCGGTCCAGGGCACCTCGATGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002173 Carbohydrate/puine kinase, PfkB, conserved site (Conserved_site) PS00583 PFKB_KINASES_1 IPR011611 PfkB (Domain) PF00294 PfkB
SignalP	No significant hit
TMHMM	No significant hit

first Bena_00020