Bena_00020 : CDS information

Location

Organism	Streptomyces sp.
Strain	A2991200
Entry name	Benastatin
Contig	AM501485
Start / Stop / Direction	2,146 / 953 / - [in whole cluster] 2,146 / 953 / - [in contig]
Location	complement(953..2146) [in whole cluster] complement(953..2146) [in contig]
Type	CDS
Length	1,194 bp (397 aa)

Annotation

Category	5.1 general function
Product	putative S-adenosylmethionine synthetase putative methionine adenosyltransferase
Product (GenBank)
Gene	benM metK
Gene (GenBank)
EC number	2.5.1.6
Keyword	SAM
Note
Note (GenBank)
Reference	PmId [17439117] Molecular analysis of the benastatin biosynthetic pathway and genetic engineering of altered fatty acid-polyketide hybrids. (J Am Chem Soc. , 2007) comment 該当UniProt entryなし。 --- Benastatin A and B生合成をコードする全遺伝子座のクローニング、シークエンス。 flanking regionsの削除とS. albus, S. lividansでの異種性発現によってclusterが同定されている。 BenM(397aa): S-adenosyl methionine synthetase methyltransferase BenFの補助基質は、BenMによっておそらく供給される。
Related Reference	ACC Q938W7 PmId [11594346] Influence of ancillary genes, encoding aspects of methionine metabolism, on tylosin biosynthesis in Streptomyces fradiae. (J Antibiot (Tokyo). , 2001) comment Blast 19th, id81%, 0.0 Streptomyces fradiae_metK S-adenosylmethionine synthase(EC 2.5.1.6) tyl clusterに含まれる補助的遺伝子・metF(N5,N10-methylenetetrahydrofolate reductase) ・orf9(unknown) ・metK(S-adenosylmethionine synthase) を削除するとtylosin産生減少。これら補助遺伝子は二次代謝の際にtransmethylation能力を拡大する。
Family/Domain	FD IPR002133 comment [IPR002133] S-adenosylmethionine synthetase (Family)

Sequence

>putative S-adenosylmethionine synthetase
MSRKLFTSESVTEGHPDKMADRISDTILDALLKEDPLSRVAVETLITTGQVHVAGEVTTT
AYADIPALVRDAVLDIGYDSSTKGFDGASCGVSVSIGAQSPDIARGVDTGVAGADRLDAQ
GAGDQGLMFGFACDETPELMPLPIRLAHRLAYRLTQVRKNGTMPYLRPDGKTQVTVEYLG
GTRVRLDTVVVSAQHAGDIDLQELLVPDVRRHVVEHELARPADDGIELDTDGFRLLVNPT
GRFHIGGPMGDAGLTGRKIVIDTYGGMARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVA
AGLASRCEVQVAYAIGRADPVGLFVETFGTATVSPTAIEKAISAVFDLRPAAIIRDLDLL
RPIYAQTAAYGHFGRELPDFTWERTDRAVALRDATGA

>putative S-adenosylmethionine synthetase
ATGTCCCGCAAACTGTTCACCTCGGAGTCCGTCACCGAGGGGCATCCCGACAAAATGGCG
GACCGGATCAGCGACACGATCCTCGACGCCCTGCTCAAGGAAGACCCCCTGTCGCGCGTC
GCCGTGGAGACCCTGATCACCACCGGCCAGGTGCACGTGGCCGGCGAGGTGACCACCACG
GCGTACGCGGACATCCCCGCCCTGGTGCGCGACGCGGTGCTCGACATCGGATACGACTCA
TCCACCAAGGGTTTCGACGGCGCCTCCTGCGGTGTCTCGGTCTCCATCGGCGCCCAGTCG
CCCGACATCGCCCGGGGTGTGGACACCGGCGTAGCGGGTGCCGACCGGCTGGACGCACAG
GGCGCCGGAGACCAGGGCCTGATGTTCGGCTTTGCCTGCGACGAGACGCCGGAGTTGATG
CCGCTGCCCATCCGGTTGGCGCATCGACTCGCGTACCGGCTGACGCAGGTCCGCAAGAAC
GGCACCATGCCGTACCTGCGCCCGGACGGAAAGACACAGGTGACGGTCGAGTACCTGGGC
GGCACCCGAGTCCGCCTCGACACCGTGGTGGTGTCGGCCCAGCACGCAGGGGACATCGAC
TTGCAGGAACTGCTGGTGCCGGACGTCCGCCGGCACGTCGTCGAGCACGAACTCGCACGG
CCGGCCGACGACGGCATCGAACTCGACACCGACGGCTTCCGGTTGCTGGTCAACCCCACC
GGCCGATTCCACATCGGTGGTCCGATGGGGGACGCGGGCCTGACCGGCCGCAAGATCGTC
ATCGACACGTACGGCGGCATGGCCCGTCACGGCGGCGGAGCCTTTTCCGGCAAGGACCCG
TCCAAGGTGGACCGCAGTGCCGCCTACGCCATGCGCTGGGTCGCCAAGAACGTGGTCGCG
GCGGGCCTGGCCTCCCGCTGCGAGGTCCAGGTCGCCTACGCCATCGGCAGGGCGGACCCG
GTCGGTCTCTTCGTGGAGACCTTCGGCACCGCCACCGTCTCCCCCACCGCGATCGAAAAG
GCCATCAGCGCCGTCTTCGACCTCCGCCCGGCCGCCATCATCCGCGACCTCGACCTGCTT
CGCCCGATCTACGCCCAGACGGCGGCGTACGGCCACTTCGGTCGCGAGCTACCGGACTTC
ACCTGGGAGCGGACCGACCGGGCCGTCGCTCTCCGCGATGCGACGGGGGCGTGA

Feature

BLASTP Database:UniProtKB:2011_09	show BLAST table
InterPro Database:interpro:38.0	IPR002133 S-adenosylmethionine synthetase (Family) TIGR01034 metK: methionine adenosyltransferase MF_00086 S_AdoMet_synth1 IPR022628 S-adenosylmethionine synthetase, N-terminal (Domain) PF00438 S-AdoMet_synt_N IPR022629 S-adenosylmethionine synthetase, central domain (Domain) PF02772 S-AdoMet_synt_M IPR022630 S-adenosylmethionine synthetase, C-terminal (Domain) PF02773 S-AdoMet_synt_C IPR022631 S-adenosylmethionine synthetase, conserved site (Conserved_site) PS00377 ADOMET_SYNTHASE_2 PS00376 ADOMET_SYNTHASE_1 IPR022636 S-adenosylmethionine synthetase superfamily (Domain) SSF55973 S-adenosylmethionine synthetase
SignalP	No significant hit
TMHMM	No significant hit

Bena_00010 Bena_00030